10 documents found, page 1 of 1

Antibiofilm and antimicrobial activity of polymyxin derivatives

Lower tract respiratory infections caused by Gram-negative pathogens classified by the World Health Organization as priority targets (e.g., Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae) pose a global public health concern as they are often mediated by biofilms, including multidrug-resistant strains [1,2]. The rise of antimicrobial resistance in these complicated infections has revived ...

New polymyxin analogs to combat Gram-negative biofilm-associated infections

Introduction: Antimicrobial resistance is a major global health threat, with which the antibiotic discovery pipeline is not keeping up. Polymyxins (PM), notably B (PMB) and E (PME) have resurrected as last-resort drugs (given their toxicity) against difficult-to-treat Gram-negative bacterial infections (often biofilm-related), but PM resistance has already been reported. In recent years, a fast-growing no. of P...

Co-immobilization of ciprofloxacin and chlorhexidine as a long-term broad-spect...

The endotracheal tube (ETT) affords support for intubated patients, but the ever-rising incidence of ventilator-associated pneumonia (VAP) is jeopardizing its application. ETT surfaces promote (poly)microbial colonization and biofilm formation, with a heavy burden for VAP. Devising safe, broad-spectrum antimicrobial materials to tackle ETT bioburden is needful. Herein, we immobilized ciprofloxacin (CIP) and/or ...

Minimum information guideline for spectrophotometric and fluorometric methods t...

The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in...

Unravelling Pseudomonas aeruginosa and Candida albicans communication in coinfe...

Modern medicine is currently facing huge setbacks concerning infection therapeutics as microorganisms are consistently knocking down every antimicrobial wall set before them. The situation becomes more worrying when taking into account that, in both environmental and disease scenarios, microorganisms present themselves as biofilm communities that are often polymicrobial. This comprises a competitive advantage, ...

Antimicrobial resistance three ways: healthcare crisis, major concepts, and the...

Worldwide, infections are resuming their role as highly effective killing diseases, as current treatments are failing to respond to the growing problem that is antimicrobial resistance (AMR). The social and economical burden of AMR seems ever rising, with health- and research-related organizations rushing to collaborate on a worldwide scale to find effective solutions. Resistant bacteria are spreading even in f...

Quantitative assessment of individual populations within polymicrobial biofilms

Selecting appropriate tools providing reliable quantitative measures of individual populations in biofilms is critical as we now recognize their true polymicrobial and heterogeneous nature. Here, plate count, quantitative real-time polymerase chain reaction (q-PCR) and peptide nucleic acid probe-fluorescence in situ hybridization (PNA-FISH) were employed to quantitate cystic fibrosis multispecies biofilms. Grow...

Discriminating between typical and atypical cystic fibrosis-related bacterial s...

This study aims to report the development of peptide nucleic acid (PNA) probes to specifically detect the cystic fibrosis (CF)-associated traditional and atypical species Pseudomonas aeruginosa and Inquilinus limosus, respectively. PNA probes were designed in silico, developed and tested in smears prepared in phosphate-buffer saline and in artificial sputum medium. A multiplex fluorescent in situ hybridization ...

Developing a model for cystic fibrosis sociomicrobiology based on antibiotic an...

Cystic fibrosis (CF) infections are invariably biofilm-mediated and polymicrobial, being safe to assume that a myriad of factors affects the sociomicrobiology within the CF infection site and modulate the CF community dynamics, by shaping their social activities, overall functions, virulence, ultimately affecting disease outcome. This work aimed to assess changes in the dynamics (particularly on the microbial c...

Clinical relevance and transcriptome studies of Pseudomonas aeruginosa biofilms