10 documents found, page 1 of 1
Brief Music Experience Questonnaire: Contribution to portuguese validation : Th...
Fonte, Carla; Silva, Isabel; Lopes, TelmaIn the Portuguese context, the assessment of music experience has been an area of growing interest, however, the lack of specific instruments suited to the culture and language is still evident. This study's main objective is to translate, adapt and validate the Brief Music Experience Questionnaire (BMEQ) for the Portuguese population. The sample consists of 369 adults, 64.5% men and 35.5% women, aged between...
Pressure drops, heat transfer coefficient, cost and power block structure for a...
Lopes, Telma; Fasquelle, Thomas; Silva, Hugo G.Direct circulation of molten salts in the solar field of parabolic trough solar power plants may be a possible breakthrough to decrease their levelized cost of electricity. While prototypes are being erected around the world, this study addresses the main concerns and changes that are related to the replacement of thermal oils by molten salts, i.e. pressure drops, heat transfer coefficient, anti-freezing soluti...
HPS2 - Demonstration of Molten-Salt in Parabolic Trough Plants -Simulation Resu...
Lopes, Telma; Fasquelle, Thomas; Silva, Hugo G.; Schmitz, K.High-Performance-Solar-2 (HPS2) is a parabolic trough demo project that is being built by a European consortium of research institutions (German Aerospace Center DLR, University of Évora) and companies (TSK-Flagsol, Rioglass, innogy SE, Steinmüller Engineering, Yara, eltherm) on the Evora Molten Salt Platform (EMSP). The purpose of this plant is to demonstrate the viability of using molten salts as heat transfe...
Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Fox...
Tavares, Barbara; Jacinto, Raquel; Sampaio, Pedro; Pestana, Sara; Pinto, Andreia; Vaz, Andreia; Roxo-Rosa, Mónica; Gardner, Rui; Lopes, TelmaFoxj1a is necessary and sufficient to specify motile cilia. Using transcriptional studies and slow-scan two-photon live imaging capable of identifying the number of motile and immotile cilia, we now established that the final number of motile cilia depends on Notch signalling (NS). We found that despite all left-right organizer (LRO) cells express foxj1a and the ciliary axonemes of these cells have dynein arms,...
Independent recruitment of Igh alleles in V(D)J recombination
Alves-Pereira, Clara F.; de Freitas, Raquel; Lopes, Telma; Gardner, Rui; Marta, Filipa; Vieira, Paulo; Barreto, Vasco M.How the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from mult...
Expression Profile of microRNAs Regulating Proliferation and Differentiation in...
Brás-Rosário, Luis; Matsuda, Alex; Pinheiro, Ana Isabel; Gardner, Rui; Lopes, Telma; Amaral, Andreia; Gama-Carvalho, MargaridaThe identification of cardiac cells with stem cell properties changed the paradigm of the heart as a post mitotic organ. These cells proliferate and differentiate into cardiomyocytes, endothelial and vascular smooth muscle cells, providing for cardiac cell homeostasis and regeneration. microRNAs are master switches controlling proliferation and differentiation, in particular regulating stem cell biology and car...
Expression profile of microRNAs regulating proliferation and differentiation in...
Rosário, Luis Brás; Matsuda, Alex; Pinheiro, Ana Isabel; Gardner, Rui; Lopes, Telma; Amaral, Andreia; Carvalho, Margarida GamaThe identification of cardiac cells with stem cell properties changed the paradigm of the heart as a post mitotic organ. These cells proliferate and differentiate into cardiomyocytes, endothelial and vascular smooth muscle cells, providing for cardiac cell homeostasis and regeneration. microRNAs are master switches controlling proliferation and differentiation, in particular regulating stem cell biology and car...
Reprogramming of DNA Methylation in Pollen Guides Epigenetic Inheritance via Sm...
Calarco, Joseph P.; Borges, Filipe; Donoghue, Mark T.A.; Van Ex, Frédéric; Jullien, Pauline E.; Lopes, Telma; Gardner, Rui; Berger, FrédéricEpigenetic inheritance is more widespread in plants than in mammals, in part because mammals erase epigenetic information by germline reprogramming. We sequenced the methylome of three haploid cell types from developing pollen: the sperm cell, the vegetative cell, and their precursor, the postmeiotic microspore, and found that unlike in mammals the plant germline retains CG and CHG DNA methylation. However, CHH...
A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones
Mata, João F.; Lopes, Telma; Gardner, Rui; Jansen, Lars E. T.Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted clones obviating the need for selection and outgrowth of drug resistant clones. Importantly, the use of a promoter-l...
FACS-based purification of Arabidopsis microspores, sperm cells and vegetative ...
Borges, Filipe; Gardner, Rui; Lopes, Telma; Calarco, Joseph P; Boavida, Leonor C; Slotkin, R; Martienssen, Robert A; Becker, Jörg DBackground: The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes a second mitosis to originate two sperm cells. The vegetative cell and the sperm cells activate distinct genetic and epigenetic mechanisms to control pollen tube growth and germ cell specifica...