24 documents found, page 1 of 3

Probe-based metagenomic pathogen detection: advancing laboratory capacity for c...

Probe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small microbial/human genetic material ratio and demanding computational resources. Here, we assessed the combined detection performance of two Illumina probe-based panels, the Respiratory and the Urinary Pathogen ID panels (RPIP and UPIP), using 99 clini...

An Overview of Monkeypox Virus Detection in Different Clinical Samples and Anal...

Mpox is a zoonotic disease caused by the Monkeypox virus (MPXV), and since May 2022, tens of thousands of cases have been reported in non-endemic countries. We aimed to evaluate the suitability of different sample types for mpox diagnostic and assess the temporal dynamics of viral load. We evaluated 1914 samples from 953 laboratory-confirmed cases. The positivity rate was higher for lesion (91.3%) and rectal sw...

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in...

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the hi...

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in...

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the hi...

Report of simultaneous measles outbreaks in two different health regions in Por...

In Portugal, measles vaccination coverage and population immunity are high, and no endemic measles cases had been reported since 2004. The World Health Organization classified measles as eliminated in the country in 2015 and 2016, based on data from the previous 3 years. However, in a context of increasing incidence in several European countries in 2016 and 2017, Portugal experienced two simultaneous measles ou...

Report of simultaneous measles outbreaks in two different health regions in Por...

In Portugal, measles vaccination coverage and population immunity are high, and no endemic measles cases had been reported since 2004. The World Health Organization classified measles as eliminated in the country in 2015 and 2016, based on data from the previous 3 years. However, in a context of increasing incidence in several European countries in 2016 and 2017, Portugal experienced two simultaneous measles ou...

Challenging measles case definition: three measles outbreaks in three Health Re...

We report three simultaneous measles outbreaks with 112 confirmed cases in three Health Regions of Portugal, from February to April 2018. The mean age of cases was 30 years, 79% worked in a healthcare setting and 87% were vaccinated. Genotype B3 was identified in 84 cases from the three outbreaks. Primary cases in each outbreak were imported. Several cases presented with modified measles, highlighting the impor...

Ulcerações Genitais Sugestivas de IST - Diagnóstico Laboratorial de Herpes Genital

Evaluation of the antiviral activity of an aqueous extract from Solidago virgau...

Herpes simplex virus type 2 (HSV-2) is widely distributed through the human population, infecting more than 500 million people globally [1]. Although typically causing mild diseases this virus may be responsible for severe infections, mainly in immunocompromised patients. Currently, there is a number of systemic antiviral agents against herpesvirus, the most commonly used being acyclovir and related drugs. Howe...

Diagnóstico por Técnicas de Biologia Molecular de Infeções do Sistema Nervoso C...