6 documents found, page 1 of 1
Spatiotemporal analysis of reported cases of acute Chagas disease in the State ...
Santos,Fred Luciano Neves; Lorena,Virginia Maria Barros de; Souza,Wayner Vieira de; Gomes,Yara de MirandaINTRODUCTION: Control strategies to eliminate the transmission of Chagas disease by insect vectors have significantly decreased the number of reported acute cases in Brazil. However, data regarding the incidence and distribution of acute Chagas disease cases in the State of Pernambuco are unavailable in the literature. METHODS: A geographical information system was used to delineate the spatiotemporal distribut...
Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with ch...
Braz,Suellen Carvalho de Moura; Lorena,Virginia Maria Barros de; Melo,Adriene Siqueira; Cavalcanti,Maria da Gloria Aureliano Melo; Gomes,Yara de MirandaIntroduction CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. Methods Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for lymphocytes at distinct time points. Results It was possib...
Chagas disease in the State of Pernambuco, Brazil: analysis of admissions and m...
Braz,Suellen Carvalho de Moura; Melo,Myllena de Fátima Alheiros Dias; Lorena,Virginia Maria Barros de; Souza,Wayner Vieira de; Gomes,Yara de MirandaINTRODUCTION: A time series study of admissions, deaths and acute cases was conducted in order to evaluate the context of Chagas disease in Pernambuco. METHODS: Data reported to the Information Technology Department of the Brazilian National Health Service between 1980 and 2008 was collected for regions and Federal Units of Brazil; and microregions and municipalities of Pernambuco. Rates (per 100,000 inhabitant...
A comparative analysis of the suitability of different peripheral blood samples...
Melo,Adriene Siqueira de; Lorena,Virginia Maria Barros de; Braz,Suellen Carvalho de Moura; Gomes,Yara de MirandaVenipuncture is one of the easiest clinical procedures to obtain viable blood samples to evaluate gene expression using mRNA analysis. However, the use of this sample type in reverse transcriptase polymerase chain reaction tests (RT-PCR) without prior treatment is controversial. We therefore propose to compare the suitability of different peripheral blood samples (whole blood without treatment, whole blood with...
Resposta atenuada ao PPD no diagnóstico de infecção tuberculosa latente em paci...
Marques,Cláudia Diniz Lopes; Duarte,Ângela Luzia Branco Pinto; Lorena,Virginia Maria Barros de; Souza,Joelma Rodrigues de; Souza,WaynerINTRODUÇÃO: A utilização dos anti-TNF's na prática reumatológica tornou obrigatória a identificação de casos de infecção tuberculosa latente (ITBL) antes do início do tratamento, utilizando o PPD, RX de tórax e história de contato com tuberculose. Os pacientes com artrite reumatoide (AR) apresentam uma anormalidade da função celular imune, caracterizada por diminuição da responsividade de células mononucleares ...
Evaluation of the immune response to CRA and FRA recombinant antigens of Trypan...
Pereira,Valéria Rêgo Alves; Lorena,Virginia Maria Barros de; Nakazawa,Mineo; Silva,Ana Paula Galvão da; Montarroyos,Ulisses; Correa-Oliveira,RodrigoHumoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compar...