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Liposomal Delivery of Newly Identified Prophage Lysins in a Pseudomonas aerugin...
Morais, Diana; Tanoeiro, Luís; Marques, Andreia; Gonçalves, Tiago; Duarte, A.; Matos, António; Vital, Joana; Cruz, Maria Eugénia MeirinhosPseudomonas aeruginosa is a Gram-negative opportunistic bacterium that presents resistance to several antibiotics, thus, representing a major threat to human and animal health. Phage-derived products, namely lysins, or peptidoglycan-hydrolyzing enzymes, can be an effective weapon against antibiotic-resistant bacteria. Whereas in Gram-positive bacteria, lysis from without is facilitated by the exposed peptidogly...
Transfection of pulmonary cells by stable pDNA-polycationic hybrid nanostructur...
Gaspar, Diana P.; Vital, Joana; Leiva, María C.; Gonçalves, Lídia; Taboada, Pablo; Remuñán-López, Carmen; Vítor, Jorge M. B.; Almeida, António JoséAim: Cationically modified solid lipid nanoparticles (SLN) were investigated as plasmid DNA (pDNA) carriers and transfection agents for the pulmonary route. Materials & methods:pDNA-loaded SLN were produced using glyceryl dibehenate or tristearate as matrix lipids and chitosan as surface charge modifier, and encapsulated by spray-drying in mannitol and trehalose microspheres. Results: Nanoparticles of 200 nm, a...
Transfection of pulmonary cells by stable pDNA-polycationic hybrid nanostructur...
Gaspar, Diana P.; Vital, Joana; Leiva, María C.; Gonçalves, Lídia M. D.; Taboada, Pablo; Remuñán-López, Carmen; Vítor, Jorge M. B.; Almeida, António J.Aim: Cationically modified solid lipid nanoparticles (SLN) were investigated as plasmid DNA (pDNA) carriers and transfection agents for the pulmonary route. Materials & methods:pDNA-loaded SLN were produced using glyceryl dibehenate or tristearate as matrix lipids and chitosan as surface charge modifier, and encapsulated by spray-drying in mannitol and trehalose microspheres. Results: Nanoparticles of 200 nm, a...
Helicobacter pylori in both the sinuses and the stomach
Borges Dinis, Paulo; Matos, Teresa; Sardinha, Miguel; Alves, Pedro Luís; Vital, Joana; Carvalho, Ana Maria; Vítor, Jorge M. B.Background: The role played by Helicobacter pylori in the sinuses, and its association with the same organism’s gastric infection, are still unclear. Methods: In order to compare H.pylori colonization patterns in the nose and stomach we conducted a cohort analysis of 14 patients, eligible for sinus surgery due to chronic medically refractory rhinosinusitis, who were tested for simultaneous presence of H. pylori...
Maternal separation impairs long term-potentiation in CA1-CA3 synapses and hipp...
Sousa, Vasco C.; Vital, Joana; Costenla, Ana Rita; Batalha, Vânia; Sebastião, Ana M; Ribeiro, Joaquim A.; Lopes, Luisa V.Exposure to chronic stress during the neonatal period is known to induce permanent long-term changes in the central nervous system and hipothalamic-pituitary-adrenal axis reactivity that are associated with increased levels of depression, anxiety, and cognitive impairments. In rodents, a validated model of early life stress is the maternal separation (MS) paradigm, which has been shown to have long-term consequ...
Genome Inspector: a web tool for exploring Bacterial genomes
Inocêncio, Tiago; Vital, Joana; Falcao, Andre; Vítor, Jorge M. B.Genome Inspector (GIN) is a new interactive web-based application for exploring bacterial genomes designed to provide users with a wide choice of options to facilitate the process of designing DNA primers for isolating genes from similar bacterial species and strains. GIN employs a project-based approach in which users can select any given set of available genomes and perform a comprehensive bioinformatics anal...
The role of predictability in the stress response of a cichlid fish
Galhardo, Leonor; Vital, Joana; Oliveira, Rui FilipeIn recent years there has been an increasing interest in the cognitive abilities of fish with implications for animal welfare and management of rearing operations. Although it is known that psychological factors can modulate the stress response in mammals, this aspect has seldom been investigated within stress in fish. In this study we investigate whether the perception (appraisal) that fish make of significant...