9 documents found, page 1 of 1
PLGA nanoparticles double-decorated with a TAT peptide and folic acid to target...
Andrade, Stéphanie; Ramalho, Maria J.; Santos, João; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Guimarães, Nuno; Ferraz, Maria P.Treating bacterial infections has become increasingly difficult due to the rise in antibiotic-resistant bacterial strains. Strategies involving the targeted delivery of antibiotics have been proposed to minimize the administered antibiotic doses. This study aims to develop the first double-modified nanovehicle capable of increasing bacterial membranes permeability while specifically targeting Staphylococcus aur...
A small TAT-TrkB peptide prevents BDNF receptor cleavage and restores synaptic ...
Fonseca-Gomes, João; Costa-Coelho, Tiago; Ferreira-Manso, Mafalda; Inteiro-Oliveira, Sara; Vaz, Sandra H.; Alemãn-Serrano, NunoIn Alzheimer's disease (AD), amyloid β (Aβ)-triggered cleavage of TrkB-FL impairs brain-derived neurotrophic factor (BDNF) signaling, thereby compromising neuronal survival, differentiation, and synaptic transmission and plasticity. Using cerebrospinal fluid and postmortem human brain samples, we show that TrkB-FL cleavage occurs from the early stages of the disease and increases as a function of pathology seve...
Nanostructured label–free electrochemical immunosensor for detection of a Parki...
Carneiro, Pedro; Loureiro, Joana A.; Delerue-Matos, Cristina; Morais, Simone; Pereira, Maria do CarmoAggregation of α-synuclein has been recognized as a critical event in the pathogenesis of Parkinson's disease whose prevalence is increasing with great socio-economic challenges for future generations. Here, we developed a sensitive and specific electrochemical immunosensor for the detection and quantification of this biomarker, based on the voltammetric study of a redox indicator signal, which decreases upon t...
Fighting methicillin-resistant staphylococcus aureus with targeted nanoparticles
Andrade, Stephanie; Ramalho, Maria J.; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Santos, Rita S.; Guimarães, Nuno; Azevedo, Nuno F.Antimicrobial resistance (AMR) is considered one of the greatest threats to global health. Methicillin-resistant Staphylococcus aureus (MRSA) remains at the core of this threat, accounting for about 90% of S. aureus infections widespread in the community and hospital settings. In recent years, the use of nanoparticles (NPs) has emerged as a promising strategy to treat MRSA infections. NPs can act directly as an...
Double optimization of rivastigmine-loaded Nanostructured Lipid Carriers (NLC) ...
Cunha, Sara; Costa, Cláudia Pina; Loureiro, Joana A.; Alves, Jorge; Peixoto, Andreia F.; Forbes, Ben; Sousa Lobo, José Manuel; Silva, Ana CatarinaRivastigmine is a drug commonly used in the management of Alzheimer's disease that shows bioavailability problems. To overcome this, the use of nanosystems, such as nanostructured lipid carriers (NLC), administered through alternative routes seems promising. In this work, we performed a double optimization of a rivastigmine-loaded NLC formulation for direct drug delivery from the nose to the brain using the qua...
Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric...
Gomes, Filipa O.; Maia, Luísa B.; Loureiro, Joana A.; Pereira, Maria Carmo; Delerue-Matos, Cristina; Moura, Isabel; Moura, José J.G.; Morais, SimoneAn enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), ...
Solvatochromism as a new tool to distinguish structurally similar compounds
Madeira, Pedro P.; Loureiro, Joana A.; Freire, Mara G.; Coutinho, João A. P.It is here reported a new concept based on solvatochromism to distinguish structurally similar compounds in aqueous solutions by the analysis of the stabilization of electronic excited states. The sensitivity of this approach to differentiate similar organic compounds, such as structural isomers or compound differing in the number of methylene groups, or proteins with conformational changes induced by being or ...
Interrelationship between partition behavior of organic compounds and proteins ...
Ferreira, Luisa A.; Silva, Nuno R.; Wlodarczyk, Samarina R.; Loureiro, Joana A.; Madeira, Pedro P.; Teixeira, J. A.; Uversky, Vladimir N.Partition behavior of adenosine and guanine mononucleotides was examined in aqueous dextran-polyethylene glycol (PEG) and PEG-sodium sulfate two-phase systems. The partition coefficients for each series of mononucleotides were analyzed as a functions of the number of phosphate groups and found to be dependent on the nature of nucleic base and on the type of \ATPS\ utilized. It was concluded that an average cont...
Interrelationship between partition behavior of organic compounds and proteins ...
Ferreira, Luisa A.; Silva, Nuno R. da; Wlodarczyk, Samarina R.; Loureiro, Joana A.; Madeira, Pedro P.; Teixeira, José A.; Uversky, Vladimir N.Partition behavior of adenosine and guanine mononucleotides was examined in aqueous dextran-polyethylene glycol (PEG) and PEG-sodium sulfate two-phase systems. The partition coefficients for each series of mononucleotides were analyzed as a functions of the number of phosphate groups and found to be dependent on the nature of nucleic base and on the type of ATPS utilized. It was concluded that an average contri...