33 documents found, page 1 of 4
Superior operational stability of immobilized l-asparaginase over surface-modif...
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.Made available in DSpace on 2022-04-28T19:46:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Fundação para a Ciência e a Tecnologia; l-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the l-asparagine hydrolysis into l-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some d...
Superior operational stability of immobilized L-asparaginase over surface-modif...
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the L-asparagine hydrolysis into L-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some disadvantages are associated with its free form, such as the ASNase short half-life, which may be overcome by enzyme immobilization. In this work, the immobilization of ASNase by adsorption over pristine and modifi...
Immobilization of L-asparaginase towards surface-modified carbon nanotubes
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C.F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes L-asparagine hydrolysis into L-aspartic acid and ammonia and is mainly applied in pharmaceutical and food industries [1]. The ASNase currently commercialized for pharmaceutical purposes is produced from two main bacterial sources: recombinant Escherichia coli and Erwinia chrysanthemi. However, some disadvantages are associated with its free form, s...
Syngas production by bi-reforming methane on an Ni–K-promoted catalyst using hy...
Cunha, Adelino F.; Morales-Torres, Sergio; Pastrana-Martínez, Luisa M.; Martins, António A.; Mata, Teresa M.; Caetano, Nídia; Loureiro, José M.Steam reforming of methane (SRM) and dry reforming of methane (DRM) are frequently used in the production of syngas; however, the bi-reforming of methane (BRM) is an interesting and alternative process. In this study, BRM was studied over MgO, a layered double hydroxide (LDH) phase that was destroyed between 600 °C and 900 °C during the reaction. It showed good sorption capacity for CO2 at relatively low temper...
Development and characterization of a novel l-asparaginase/MWCNT nanobioconjugate
Cristóvão, Raquel O.; Almeida, Mafalda R.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.The enzyme L-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient immobilization techniques is in high demand. Carbon nanotubes are promising enzyme immobilization supports, sinc...
Fish canning industry wastewater variability assessment using multivariate stat...
Cristovão, Raquel; Pinto, Victor Miguel da Silva; Gonçalves, António; Martins, Ramiro; Loureiro, José M.; Boaventura, RuiUsually, fish canning industrial wastewaters have a highly variable composition over time. For a good performance of treatment processes it is necessary to limit that variation. However, extended wastewater monitoring, including all relevant analytical parameters, is expensive. This work proposes an efficient approach to minimize the analytical determina-tions number without compromising the global characteriza...
Assessing the influence of oil and grease and salt content on fish canning wast...
Cristovão, Raquel; Pinto, Vera; Martins, Ramiro; Loureiro, José M.; Boaventura, RuiFish canning industry wastewaters have high organic matter, oil and grease and salt (NaCl) content, which make difficult a proper treatment before discharge. In this work, their treatment was evaluated via activated sludge aerobic biological process through respirometric tests. Inhibition was found to be significant for NaCl concentr ations higher than 17.5 g/L. On the other hand, the oil and grease content aff...
Fish canning wastewater treatment by activated sludge: Application of factorial...
Cristovão, Raquel; Gonçalves, Cristiana; Botelho, Cidália; Martins, Ramiro; Loureiro, José M.; Boaventura, RuiThe optimization of hydraulic retention time (HRT) and initial organic matter concentration for dissolved organic carbon (DOC) abatement of wastewater from a fish canning industry of northern Portugal by activated sludge was investigated using response surface methodology (RSM). The two parameters were chosen since it was found that the treatment efficiency is mainly influenced by them. The experimental data on...
Detection of fish industry wastewater main pollution sources by cluster analysis
Cristovão, Raquel; Martins, Ramiro; Loureiro, José M.; Boaventura, RuiWater consumption in fish canning industry and the production of high polluted wastewaters are of great concern worldwide. In fact, in this type of industry a huge amount of water is used throughout all steps, including cleaning, cooking, cooling, sanitization and floor washing. It is also known a frequent change of raw material to be processed, with variation in flow rates and composition, which implies change...
Fish canning industry wastewater treatment for water reuse – a case study
Cristovão, Raquel; Botelho, Cidália; Martins, Ramiro; Loureiro, José M.; Boaventura, RuiThe valorization of wastewaters from the fish canning industry is of great concern, not only because of the high quantities generated, but also economic and environmental benefits may result from a proper treatment approach of the waste generated while reducing costs related to wastewater discharge. A limiting factor for reuse and recycling treated fish canning wastewater into an industrial plant and also for o...