15 documents found, page 1 of 2
Proliferation and cell death in an experimental model of brain tissue heterotop...
Quemelo,Paulo Roberto Veiga; Peres,Luiz CesarPURPOSE: To investigate the proliferation and neuronal death in brain tissue heterotopia in the lung in an experimental model during both fetal and neonatal periods. METHODS: Twenty four pregnant female Swiss mice were used to induce brain tissue heterotopia on the 15th gestational day. Briefly, the brain of one fetus of each dam was extracted, disaggregated and injected into the right hemithorax of siblings. S...
Sacarose como veículo de suplementação dietética de ácido fólico em camundongos...
Lima,Giovanna Arcaro de; Gutierrez,Cristiane Minot; Yanaguita,Maira Yuli; Ribeiro,Camila Nunes de Morais; Machado,Hélio Rubéns; Peres,Luiz CesarINTRODUÇÃO: A ingestão adequada de folato é essencial durante a embriogênese, e sua deficiência está associada à ocorrência de defeitos no fechamento do tubo neural. OBJETIVO: Determinar se a sacarose é um bom veículo para a suplementação de folato em camundongos. MATERIAL E MÉTODOS: Quarenta camundongos Swiss fêmeas foram divididos nos grupos: C: ração comercial + água ad libitum; DS: ração balanceada isenta d...
Influence of amplicon size on the polymerase chain reaction of Parvovirus B19 g...
Quemelo,Paulo Roberto Veiga; Fonseca,Benedito Antônio Lopes da; Lima,Danielle Malta; Peres,Luiz CesarThe polymerase chain reaction (PCR) has provided diagnosis of archival material, but some fixation methods such as formalin damage DNA and, subsequently, affect PCR analysis, particularly paraffin-embedded tissues. PCR is known due to its high specificity and sensitivity, although some difficulties arise when formalinfixed and paraffin-embedded tissue is used. Not only does this occur due to protein cross-linki...
Effect of alloxan-induced diabetes mellitus and ethanol on pregnancy outcome in...
Peres,Luiz Cesar; Ribeiro,Camila Nunes de Morais; Gutierrez,Cristiane Minot; Foss,Milton CesarINTRODUCTION AND OBJECTIVES: To investigate the effects of ethanol, diabetes mellitus and the combination of both on mouse fetuses. METHODS: We used 24 female Swiss mice, dividing them into four groups of 6 each: control (C), ethanol (E), diabetes (D) (blood glucose > 200 mg/dL) and diabetes + ethanol (DE). Diabetes was induced by alloxan (40 mg/kg) on day 7 of pregnancy. Groups E and DE received 4 g/kg of 25% ...
Sudden infant death syndrome in Brazil: fact or fancy?
Woida,Francesca Maia; Saggioro,Fabiano Pinto; Ferro,Maria Alice Rossato; Peres,Luiz CesarCONTEXT AND OBJECTIVE: The true incidence of sudden infant death syndrome (SIDS) in Brazil is unknown. The aim here was to identify SIDS cases in the city of Ribeirão Preto, State of São Paulo, between 2000 and 2005, in order to estimate its incidence. DESIGN AND SETTING: Retrospective analysis of data on live births and infant deaths in Ribeirão Preto and from autopsies of infants performed at the Death Verifi...
Teratogenic effect of retinoic acid in swiss mice
Quemelo,Paulo Roberto Veiga; Lourenço,Charles Marques; Peres,Luiz CesarPURPOSE: To identify the types of malformations resulting from the administration of retinoic acid (RA) to Swiss mice on different days of pregnancy. METHODS: Twenty-four pregnant Swiss mice were divided into 4 groups of 6 animals each. The experimental groups received a single intraperitoneal injection of RA (70 mg/kg) on gestational days 7, 8 and 9 (D7, D8 and D9), while control animals (C) received only sali...
Brain tissue aspiration neural tube defect
Peres,Luiz Cesar; Barbosa,Gustavo Henrique T. de SalesThe study aimed to find out how frequent is brain tissue aspiration and if brain tissue heterotopia could be found in the lung of human neural tube defect cases. Histological sections of each lobe of both lungs of 22 fetuses and newborn with neural tube defect were immunostained for glial fibrillary acidic protein (GFAP). There were 15 (68.2%) females and 7 (31.8%) males. Age ranged from 18 to 40 weeks of gesta...
Avaliação de uma Reestruturação Curricular na Faculdade de Medicina de Ribeirão...
Troncon,Luiz Ernesto de Almeida; Figueiredo,José Fernando Castro; Rodrigues,Maria de Lourdes Veronese; Piccinato,Carlos Eli; Peres,Luiz CesarResumo: Avaliou-se a eficácia de um novo currículo introduzido na Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo) com base no desempenho dos graduandos em provas objetivas que abrangeram os domínios cognitivo, psicomotor e afetivo. A avaliação foi aplicada durante seis anos a 222 formando do antigo currículo e 261 graduandos do novo currículo. Todos se submeteram a um prova de conhecimentos ...
Enamel hypoplasia in a litter of rats with alloxan-induced diabetes mellitus
Silva-Sousa,Yara Teresinha Corrêa; Peres,Luiz Cesar; Foss,Milton CesarEnamel hypoplasia is an important clinical problem commonly seen in children born to diabetic women. We aimed to characterize the enamel hypoplasia in Wistar rats born to alloxan-induced diabetes mellitus rats. Groups consisted of pregnant rats supplemented (ISDR) or not (NISDR) with insulin and controls, in which sterile saline solution was administered instead of alloxan or insulin. The mandibular incisors of...
Are there structural alterations in the enamel organ of offspring of rats with ...
Silva-Sousa,Yara Teresinha Corrêa; Peres,Luiz Cesar; Foss,Milton CésarEnamel hypoplasia is the most common developmental defect of human teeth that may be seen in deciduous teeth of babies born to diabetic women. In the present experimental study, we analyzed the enamel organ of the mandibular incisors of the offspring of rats with alloxan-induced diabetes. By light microscopy, no alterations could be found in the enamel organ of rats born to diabetic mothers compared to normal o...