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Hydrogel bioinks of alginate and curcumin-loaded cellulose ester-based particle...

Carvalho, João P. F.; Teixeira, Maria C.; Lameirinhas, Nicole S.; Matos, Filipe S.; Luís, Jorge L.; Pires, Liliana; Oliveira, Helena; Oliveira, Martinho

3D bioprinting is a versatile technique that allows the fabrication of living tissue analogs through the layer-by-layer deposition of cell-laden biomaterials, viz. bioinks. In this work, composite alginate hydrogel-based bioinks reinforced with curcumin-loaded particles of cellulose esters (CEpCUR) and laden with human keratinocytes (HaCaT) are developed. The addition of the CEpCUR particles, with sizes of 740 ...


Nanofibrillated cellulose/gellan gum hydrogel-based bioinks for 3D bioprinting ...

Lameirinhas, Nicole S.; Teixeira, Maria C.; Carvalho, João P.F.; Valente, Bruno F.A.; Pinto, Ricardo J.B.; Oliveira, Helena; Luís, Jorge L.

The development of suitable bioinks is an important research topic in the field of three-dimensional (3D) bioprinting. Herein, novel hydrogel-based bioinks composed of nanofibrillated cellulose (NFC) and gellan gum (GG) in different NFC/GG mass proportions (90:10, 80:20, 70:30, and 60:40) were developed and characterized. The increase in the content of GG, as well as its combination with NFC, enhanced their rhe...


Freeform 3D printing using a continuous viscoelastic supporting matrix

Patrício, Sónia Gonçalves; Sousa, Liliana R.; Correia, Tiago Ruivo; Gaspar, Vítor M.; Pires, Liliana S.; Luís, Jorge L.; Oliveira, José Martinho

Embedded bio-printing has fostered significant advances toward the fabrication of soft complex tissue-like constructs, by providing a physical support that allows the freeform shape maintenance within the prescribed spatial arrangement, even under gravity force. Current supporting materials still present major drawbacks for up-scaling embedded 3D bio-printing technology towards tissue-like constructs with clini...


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