6 documents found, page 1 of 1
Care as a Method and Methods for Care: Researching Multispecies Relationships i...
Bankovska, Agnese; Lukasik, KarolinaCare as an ethical guide and practice is paramount in multispecies relationships in urban gardens. This article contributes to the discussion on the diversity of care in urban gardens. We assert that the choice and combination of methodological approaches play a crucial role in noticing and acknowledging new forms of care among multispecies gardeners. Furthermore, a better understanding of care in urban gardens...
Enzymes present in neutrophil extracellular traps may stimulate the fibrogenic ...
Rebordão, Maria Rosa; Amaral, Ana; Fernandes, Carina; Silva, E; Lukasik, Karolina; Szóstek-Mioduchowska, Anna; Bravo, Pedro; Galvão, AntónioEndometrosis is a fibrotic disease in mare endometrium whose pathological mechanisms remain obscure. Prostaglandin (PG)F2α, despite modulating reproductive physiological processes, may also provoke local pathological collagen deposition (fibrogenesis). Neutrophil extracellular traps (NETs) released during inflammation have been linked to fibrogenesis in several tissues. We have previously shown that enzymes fou...
The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen...
Amaral, Ana; Fernandes, Carina; Szostek-Mioduchowska, Anna; Lukasik, Karolina; Rebordão, Maria Rosa; Pinto-Bravo, Pedro; Skarzynski, Dariusz JanABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and a...
Myeloperoxidase inhibition decreases the expression of collagen and metallopept...
Amaral, Ana; Fernandes, Carina; Rebordão, Maria Rosa; Szostek-Mioduchowska, Anna; Lukasik, Karolina; Pinto-Bravo, Pedro; Gama, LuisABSTRACT - Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives of this in vitro work were to investigate, in explants of mare’s endometrium, ...
The In vitro inhibitory effect of sivelestat on elastase induced collagen and m...
Amaral, Ana; Fernandes, Carina; Rebordão, Maria Rosa; Szóstek-Mioduchowska, Anna; Lukasik, Karolina; Gawronska-Kozak, Barbara; Gama, LuisAbstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium. Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and gelatinolytic activity, as well as ...
The inhibition of Cathepsin G on endometrial explants with endometrosis in the ...
Amaral, Ana; Fernandes, Carina; Faes Morazzo, Sofia; Rebordão, Maria Rosa; Szóstek-Mioduchowska, Anna; Lukasik, Karolina; Gawronska-Kozak, BarbaraAlthough proteases found in neutrophil extracellular traps (NETs) have antimicrobial properties, they also stimulate collagen type 1 (COL1) production by the mare endometrium, contributing for the development of endometrosis. Cathepsin G (CAT), a protease present in NETs, is inhibited by specific inhibitors, such as cathepsin G inhibitor I (INH; beta-keto-phosphonic acid). Matrix metallopeptidases (MMPs) are pr...