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Ionic liquids toward enhanced carotenoid extraction from bacterial biomass

Silva, Tiago; Alves, Luís; Salgado, Francisco; Roseiro, J. Carlos; Lukasik, Rafal M.; Paixão, Susana M.

ABSTRACT: Carotenoids are high added-value products primarily known for their intense coloration and high antioxidant activity. They can be extracted from a variety of natural sources, such as plants, animals, microalgae, yeasts, and bacteria. Gordonia alkanivorans strain 1B is a bacterium recognized as a hyper-pigment producer. However, due to its adaptations to its natural habitat, hydrocarbon-contaminated so...

Date: 2024   |   Origin: Repositório do LNEG

Optimizing deep eutectic solvent pretreatment for enhanced glucan recovery from...

Piedade, Patrícia J.; Nowotarski, Michal M.; Dudek, Gabriela; Lukasik, Rafal M.

ABSTRACT: This work focuses on the use of the deep eutectic solvent composed of choline chloride and acetic acid (1 : 2) in the pretreatment of the perennial energy crop Miscanthus, commonly called silvergrass. The pretreatment time and temperature were optimized to achieve a maximum of the glucan and minimum of the lignin contents in the pretreated biomass. In the optimization approach, the maximal glucan reco...

Date: 2024   |   Origin: Repositório do LNEG

Ionic liquids toward enhanced carotenoid extraction from bacterial biomass

Silva, Tiago; Alves, Luís; Salgado, Francisco; Roseiro, J. Carlos; Lukasik, Rafal M.; Paixão, Susana M.

ABSTRACT: Carotenoids are high added-value products primarily known for their intense coloration and high antioxidant activity. They can be extracted from a variety of natural sources, such as plants, animals, microalgae, yeasts, and bacteria. Gordonia alkanivorans strain 1B is a bacterium recognized as a hyper-pigment producer. However, due to its adaptations to its natural habitat, hydrocarbon-contaminated so...

Date: 2024   |   Origin: Repositório do LNEG

Comprehensive Wheat Straw Processing with Deep Eutectic Solvent to Deliver Redu...

Piedade, Patrícia J.; Venkat, Veshal; Al-Shwafy, Khaled W. A.; Aregawi, Mearg A.; Dudek, Gabriela; Zygadlo, Mateusz; Lukasik, Rafal M.

ABSTRACT: Pretreatment is one of the bottlenecks in the cost and energy-efficient biomass valorization. Deep eutectic solvents are potential candidates for being used to address these challenges. In this work, the deep eutectic solvent composed of choline chloride, and acetic acid was studied for its use in wheat straw fractionation. The pretreated biomass was assessed concerning the lignin and glucan content. ...

Date: 2024   |   Origin: Repositório do LNEG

Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production

Fontes, Afonso; Francisco, Ricardo; Ferreira, Frederico Castelo; Faria, Nuno Torres; Marques, Susana; Reis, Alberto; Moura, Patrícia; Lukasik, Rafal M.

ABSTRACT: Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further culti...

Date: 2024   |   Origin: Repositório do LNEG

Mapping municipal solid waste to boost circular valorization practices in Łódzkie

Moura, Patrícia; Eusebio, Ana; Patinha, Pedro; Quental, Lídia; Lukasik, Rafal M.

ABSTRACT: Geographic Information System (GIS) is a powerful instrument that can be used for the spatial representation of waste and by-product flows at various levels, allowing to improve municipal solid waste (MSW) management. The mapping obtained can be advantageously targeted to build a regional network of technological, economic, social and environmental linkages and to boost circular economy practices. In ...

Date: 2023   |   Origin: Repositório do LNEG

Development of the operational model and methodology of collecting data, updati...

Kochanska, Ewa; Adamkiewicz, Iwona; Wozniak, Katarzyna; Przygodzki, Zbigniew; Trippner-Hrabi, Justyna; Feltynowski, Marcin; Szafranska, Ewa

ABSTRACT: The overall objective of the FRONTSH1P project is to ensure the green and just transition of the Polish Lodzkie Region towards decarbonization and territorial regeneration through demonstration at TRL7 of four Circular Systemic Solutions (CSS), interconnected one each other and facing the identified regional challenges/opportunities. FRONTSH1P systemic approach will be enabled by a circular governance...

Date: 2023   |   Origin: Repositório do LNEG

Global Ban on Plastic and What Next? Are Consumers Ready to Replace Plastic wit...

Kochanska, Ewa; Wozniak, Katarzyna; Nowaczyk, Agnieszka; Piedade, Patrícia J.; Lavorato, Marilena Lino de Almeida; Almeida, Alexandre Marcelo

ABSTRACT: Plastic can be used for many things and at the same time is the most versatile material in our modern world. However, the uncontrolled and unprecedented use of plastic comes to its end. The global ban on plastic brings significant changes in technology but even more so in civil perception-changes taking place before our eyes. The aim of this study was to find answers to the questions about the readine...

Date: 2022   |   Origin: Repositório do LNEG

Development of an ammonia pretreatment that creates synergies between biorefine...

Morais, Ana Rita C.; Zhang, Jian; Dong, Hui; Otto, William G.; Mokomele, Thapelo; Hodge, David; Balan, Venkatesh; Dale, Bruce E.; Lukasik, Rafal M.

ABSTRACT: A novel ammonia-based pretreatment for densified lignocellulosic biomass was developed to reduce ammonia usage and integrate with viable biomass logistics scenarios. The COmpacted Biomass with Recycled Ammonia (COBRA) pretreatment performed at 100 degrees C allows >95% conversion of sugarcane bagasse (SCB) carbohydrates into soluble monomeric and oligomeric sugars (glucose and xylose) using industrial...

Date: 2022   |   Origin: Repositório do LNEG

Application of ionic liquids for bacterial carotenoid extraction

Salgado, Francisco; Silva, Tiago; Alves, Luís; Roseiro, J. Carlos; Lukasik, Rafal M.; Paixão, Susana M.

ABSTRACT: One of the ways to make microbial bioprocesses more economically viable is to enhance valorization of high added value products resulting from the biomass, like carotenoids, which have a high market value. To recover these pigments from microbial biomass a good extraction method is required. Solvent extraction is one of the methods commonly used to extract carotenoids, however, solvent extractions are...

Date: 2022   |   Origin: Repositório do LNEG

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