5 documents found, page 1 of 1
DNA damage in circulating leukocytes measured with the comet assay may predict ...
Bonassi, S; Ceppi, M; Møller, P; Azqueta, A; Milić, M; Neri, M; Brunborg, G; Godschalk, R; Koppen, G; Langie, SAS; Teixeira, JP; Bruzzone, MThe comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was fol...
The hCOMET project: International database comparison of results with the comet...
Milić, M; Ceppi, M; Bruzzone, M; Azqueta, A; Brunborg, G; Godschalk, R; Koppen, G; Langie, S; Møller, P; Teixeira, JP; Alija, A; Anderson, D; Andrade, VThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent...
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations f...
Møller, P; Azqueta, A; Boutet-Robinet, E; Koppen, G; Bonassi, S; Milić, M; Gajski, G; Costa, S; Teixeira, JP; Costa Pereira, C; Dusinska, MThe comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here...
Potassium bromate as positive assay control for the Fpg-modified comet assay
Møller, P; Muruzabal, D; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Langie, SAS; Azqueta, A; Jensen, A; Scavone, F; Giovannelli, LThe comet assay is a popular assay in biomonitoring studies. DNA strand breaks (or unspecific DNA lesions) are measured using the standard comet assay. Oxidative stress-generated DNA lesions can be measured by employing DNA repair enzymes to recognise oxidatively damaged DNA. Unfortunately, there has been a tendency to fail to report results from assay controls (or maybe even not to employ assay controls). We b...
Nanomaterials Versus Ambient Ultrafine Particles: An Opportunity to Exchange To...
Stone, V; Miller, MR; Clift, MJD; Elder, A; Mills, NL; Møller, P; Schins, RPF; Vogel, U; Kreyling, WG; Jensen, KA; Kuhlbusch, TAJ; Schwarze, PE; Hoet, PBackground: A rich body of literature exists that has demonstrated adverse human health effects following exposure to ambient air particulate matter (PM), and there is strong support for an important role of ultrafine (nanosized) particles. At present, relatively few human health or epidemiology data exist for engineered nanomaterials (NMs) despite clear parallels in their physicochemical properties and biologi...