21 documents found, page 1 of 3
The comet assay as a tool in human biomonitoring studies: effects of confoundin...
Møller, Peter; Gajski, Goran; Gerić, Marko; Giovannelli, Lisa; Azqueta, Amaya; Haveric, Anja; Stopper, Helga; Bankoglu, Ezgi Eyluel; Collins, AndrewThe comet assay is widely used in human biomonitoring studies of environmental and occupational exposures. However, it is clear from multiple studies that various types of confounding factors might affect the direct relationship between exposure and DNA damage in the comet assay. In addition to common confounders such as age, sex, and smoking, other factors considered to be important determinants for background...
The comet assay as a tool in human biomonitoring of exposure to heavy metals: a...
Møller, Peter; Bankoglu, Ezgi Eyluel; Stopper, Helga; Gajski, Goran; Gerić, Marko; Haveric, Anja; Azqueta, Amaya; Giovannelli, Lisa; Collins, AndrewExposure to heavy metals such as lead, arsenic, and chromium is associated with genotoxicity and increased risk of cancer. In this systematic review and meta-analysis, we have assessed the effects of heavy metal exposure on levels of DNA strand breaks in leukocytes, measured by the comet assay, in human biomonitoring studies. We distinguish between traditional toxic metals (lead), semi-metals/metalloids (arseni...
The comet assay as a tool in human biomonitoring exposure to pesticides: a syst...
Gerić, Marko; Azqueta, Amaya; Møller, Peter; Bankoglu, Ezgi Eyluel; Gajski, Goran; Giovannelli, Lisa; Haverić, Anja; Stopper, Helga; Collins, AndrewExposure to pesticides, most usually in occupational settings, is associated with different adverse health effects. In this systematic review and meta-analysis, we have assessed the effects of pesticide exposure on the level of DNA strand breaks in human peripheral blood cells, measured by the comet assay, in human biomonitoring studies. The literature search led to 80 studies included in the review. Of these, ...
The comet assay as a tool in human biomonitoring studies of environmental and o...
Ladeira, Carina; Møller, Peter; Giovannelli, Lisa; Gajski, Goran; Haveric, Anja; Bankoglu, Ezgi Eyluel; Azqueta, Amaya; Gerić, Marko; Stopper, HelgaBiomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens may enable the detection of damage and early disease prevention. In recent years, the comet assay has become an important tool for assessing DNA damage, both in environmental and occupational exposure contexts. To evidence the role of the comet assay in human biomonitoring, we have analysed origin...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to humans. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers, some chemically induced DNA adducts. Depending on the specimen type, there ar...
Long-term cryopreservation of potassium bromate positive assay controls for mea...
Møller, Peter; Azqueta, Amaya; Rodriguez-Garraus, Adriana; Bakuradze, Tamara; Richling, Elke; Bankoglu, Ezgi Eyluel; Stopper, HelgaThe formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay is widely used for the measurement of oxidatively generated damage to DNA. However, there has not been a recommended long-term positive control for this version of the comet assay. We have investigated potassium bromate as a positive control for the Fpg-modified comet assay because it generates many Fpg-sensitive sites with a little concurrent g...
DNA strand break levels in cryopreserved mononuclear blood cell lines measured ...
Møller, Peter; Azqueta, Amaya; Rodriguez-Garraus, Adriana; Bakuradze, Tamara; Richling, Elke; Bankoglu, Ezgi Eyluel; Stopper, HelgaThe comet assay is widely used in biomonitoring studies for the analysis of DNA damage in leukocytes and peripheral blood mononuclear cells. Rather than processing blood samples directly, it can be desirable to cryopreserve whole blood or isolated cells for later analysis by the comet assay. However, this creates concern about artificial accumulation of DNA damage during cryopreservation. In this study, 10 labo...
Visual comet scoring revisited: a guide to scoring comet assay slides and obtai...
Møller, Peter; Azqueta, Amaya; Sanz-Serrano, Julen; Bakuradze, Tamara; Richling, Elke; Eyluel Bankoglu, Ezgi; Stopper, Helga; Claudino Bastos, VictoriaMeasurement of DNA migration in the comet assay can be done by image analysis or visual scoring. The latter accounts for 20%-25% of the published comet assay results. Here we assess the intra- and inter-investigator variability in visual scoring of comets. We include three training sets of comet images, which can be used as reference for researchers who wish to use visual scoring of comets. Investigators in 11 ...
Inter-laboratory variation in measurement of DNA damage by the alkaline comet a...
Møller, Peter; Azqueta, Amaya; Collia, Miguel; Bakuradze, Tamara; Richling, Elke; Bankoglu, Ezgi Eyluel; Stopper, Helga; Bastos, Victoria ClaudinoThe comet assay is a simple and versatile method for measurement of DNA damage in eukaryotic cells. More specifically, the assay detects DNA migration from agarose gel-embedded nucleoids, which depends on assay conditions and the level of DNA damage. Certain steps in the comet assay procedure have substantial impact on the magnitude of DNA migration (e.g. electric potential and time of electrophoresis). Inter-l...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...