2 documents found, page 1 of 1
Comparison of protocols for removal of melanin from genomic DNA to optimize PCR...
Vicente, Anna Luiza Silva Almeida; Bianchini, Raquel Alves; Laus, Ana Carolina; Macedo, Graziela; Reis, R. M.; Vazquez, Vinicius de LimaMelanin is produced by melanocytes and protects against DNA damage by ultraviolet light. Unfortunately, the melanin protein present in melanoma tumor cells is often co-purified during DNA extraction, and this contamination may inhibit subsequent PCR methods, which directly impacts research applications and the molecular diagnostic tests needed for targeted therapeutics. There are presently no described purifica...
Mutational profile of driver genes in Brazilian melanomas
Vicente, Anna Luiza S. A.; Crovador, Camila S.; Macedo, Graziela; Scapulatempo-Neto, Cristovam; Reis, R. M.; Vazquez, Vinicius L.Mutation testing of the key genes involved in melanoma oncogenesis is now mandatory for the application of targeted therapeutics. However, knowledge of the mutational profile of melanoma remains largely unknown in Brazil. PURPOSE Mutation testing of the key genes involved in melanoma oncogenesis is now mandatory for the application of targeted therapeutics. However, knowledge of the mutational profile of melano...