11 documents found, page 1 of 2
Chemical inhibition of β-glucocerebrosidase does not affect phagocytosis and ea...
Ribeiro, H.; Rocha, M. I.; Castro, H.; Macedo, M. F.Gaucher disease is a lysosomal storage disease in which a genetic deficiency in β-glucocerebrosidase leads to the accumulation of glycosphingolipids in lysosomes. Macrophages are amongst the cells most severely affected in Gaucher disease patients. One phenotype associated with Gaucher macrophages is the impaired capacity to fight bacterial infections. Here, we investigate whether inhibition of β-glucocerebrosi...
Characterization of the glaze and in-glaze pigments of the nineteenth-century r...
Coutinho, M. L.; Veiga, J. P.; Mirao, Jose; Dias, L.; Lima, A. M.; Muralha, V. S.; Macedo, M. F.The glaze and in-glaze pigments of the historical nineteenth-century glazed tiles from the Pena National Palace (Sintra, Portugal) were characterized using a multi-analytical approach. Chemical composition and microstructural characterization were ascertained by µ-PIXE, µ-Raman, optical microscopy and VP-SEM–EDS. The manufacturing technique and colour palette in these tiles were found to be close to the ceramic...
An integrated approach for assessing the bioreceptivity of glazed tiles to phot...
Coutinho, M. L.; Miller, A. Z.; Martin-Sanchez, P. M.; Mirao, J.; Gomez-Bolea, A.; Machado-Moreira, B.; Cerqueira-Alves, L.; Jurado, V.A laboratory-based methodology was designed to assess the bioreceptivity of glazed tiles. The experimental set-up consisted of multiple steps: manufacturing of pristine and artificially aged glazed tiles, enrichment of phototrophic microorganisms, inoculation of phototrophs on glazed tiles, incubation under optimal conditions and quantification of biomass. In addition, tile intrinsic properties were assessed to...
A Multidisciplinary Approach to the Study of Archaeological Mortars from the To...
Cardoso, I.; Macedo, M. F.; Vermeulen, F.; Corsi, C.; Santos Silva, A.; Rosado, L.; Candeias, A.; Mirao, J.he Roman town of Ammaia (in Marvão Region) is considered one of the most important recent findings of the Roman presence in Portuguese territory. It was settled in Republican times and abandoned in the seventh century. In this research, 17 masonry mortars and renders from the West Tower (South Gate), the residential area near the West Tower, the macellum, the peristylium, the public bath building, the podium of...
Risk analysis in a Portuguese archive what has changed in five years?
Pinheiro, A. C.; Moura, L.; Sequeira, Sílvia; Viegas, Carla; Viegas, Susana; Macedo, M. F.The Cultural Property Risk Analysis Model was applied in 2006 to a Portuguese archive located in Lisbon. Its results highlighted the need for the institution to take care of risks related to fire, physical forces and relative humidity problems. Five years after this first analysis the results are revisited and a few changes are introduced due to recent events: fire and high humidity remain an important hazard b...
Técnicas no destructivas para la monitorización cuantitativa y cualitativa de p...
Rogerio-Candelera, M. A.; Miller, A. Z.; Dionísio, A.; Macedo, M. F.; Saiz-Jiménez, C.Biological stone colonisation is one of the main problems related to the conservation of monuments and buildings. Stone biodeterioration has been assessed by several authors using time-consuming and extensive sampling methodologies for microbial identification and the follow-up of their action. However, most sampling procedures may convert the own researchers in deterioration agents. In this study the use of no...
Indoor air quality in Portuguese archives: a snapshot on exposure levels
Pinheiro, A. C.; Viegas, Carla; Viegas, Susana; Veríssimo, C.; Brandão, J.; Macedo, M. F.Indoor air quality recently entered legislation in Portugal. Several parameters must be evaluated and kept within limits in order to obtain a certification for air quality and energy consumption. Certification parameters were analyzed in two Portuguese archives in order to assess indoor air quality both for people attending or working on these premises and for maintenance of a written heritage that must be reta...
Estudo de comunidades fúngicas em arquivos: implicações na conservação e na saúde
Pinheiro, A. C.; Viegas, Carla; Jurado, V.; Sabino, Raquel; Veríssimo, Carlos; Rosado, Laura; Macedo, M. F.A influência da contaminação fúngica para a saúde ambiental e para a conservação do património é o tema premente e actual que suscitou a hipótese de estudo aqui apresentada. Os fungos, dada a sua extrema capacidade de adaptação, podem colonizar diversos materiais - orgânicos ou não - e a sua acção pode ser mecânica, por intermédio das suas hifas, ou química, através dos seus metabolitos. Em termos de conservaçã...
Mould and yeast identification in archival settings: preliminary results on the...
Pinheiro, A. C.; Macedo, M. F.; Jurado, V.; Saiz-Jimenez, C.; Viegas, Carla; Brandão, J.; Rosado, L.This project was developed to fully assess the indoor air quality in archives and libraries from a fungal flora point of view. It uses classical methodologies such as traditional culture media – for the viable fungi – and modern molecular biology protocols, especially relevant to assess the non-viable fraction of the biological contaminants. Denaturing high-performance liquid chromatography (DHPLC) has emerged ...
Laboratory-induced endolithic growth in calcarenites: biodeteriorating potentia...
Miller, A. Z.; Rogerio-Candelera, M. A.; Laiz, L.; Wierzchos, J.; Ascaso, C.; Braga, M. A. Sequeira; Hernández-Mariné, M.; Maurício, A.; Dionísio, A.This study is aimed to assess the formation of photosynthetic biofilms on and within different natural stone materials, and to analyse their biogeophysical and biogeochemical deterioration potential. This was performed by means of artificial colonisation under laboratory conditions during 3 months. Monitoring of microbial development was performed by image analysis and biofilm biomass estimation by chlorophyll ...