30 documents found, page 1 of 3
Towards clinical breakpoints for non-tuberculous mycobacteria – Determination o...
EUCAST AMST study group; ESCMYC study group; Viveiros, Miguel; Machado, DianaFunding Information: FPM; Mukoviszidose Institut gGmbH, Bonn, the German Cystic Fibrosis Association Mukoviszidose e.V. TS; Swedish Heart and Lung Foundation and the Swedish research council. Funding Information: The Clinical and Laboratory Standards Institute (CLSI) recommends using broth microdilution (BMD) in cation adjusted Mueller Hinton broth (CAMHB) for AST of most NTM [10,11]. There is limited data in s...
Balancing access to BPaLM regimens and risk of resistance
Van Rie, Annelies; Walker, Timothy; de Jong, Bouke; Rupasinghe, Praharshinie; Rivière, Emmanuel; Dartois, Véronique; Sonnenkalb, Lindsay; Machado, DianaFunding Information: AVR reports receiving funding from Research Foundation Flanders (Belgium) related to whole-genome sequencing of M tuberculosis. BdJ reports an institutional contract for central laboratory support to the C211 pediatric bedaquiline trial with Janssen and participation in the scientific advisory boards of the Dioraphte foundation and Panacea. PS reports personal consulting fees from Genoscree...
Ancient and recent differences in the intrinsic susceptibility of Mycobacterium...
Bateson, Anna; Canseco, Julio Ortiz; McHugh, Timothy D.; Witney, Adam A.; Feuerriegel, Silke; Merker, Matthias; Kohl, Thomas A.; Utpatel, ChristianObjectives: To develop a robust phenotypic antimicrobial susceptibility testing (AST) method with a correctly set breakpoint for pretomanid (Pa), the most recently approved anti-tuberculosis drug. Methods: The Becton Dickinson Mycobacterial Growth Indicator Tube™ (MGIT) system was used at six laboratories to determine the MICs of a phylogenetically diverse collection of 356 Mycobacterium tuberculosis complex (M...
In vitro Evaluation of Isoniazid Derivatives as Potential Agents Against Drug-R...
Marquês, Joaquim Trigo; Frazão De Faria, Catarina; Reis, Marina; Machado, Diana; Santos, Susana; Santos, Maria da Soledade; Viveiros, MiguelFunding Information: Financed by Fundação para a Ciência e a Tecnologia, I.P./MCTES through national funds (PIDDAC, PT2020) under projects PTDC/MED-QUI/29036/2017, PTDC/BIA-MIC-30692/2017, EXPL/BIA-BFS/1034/2021, UIDB/00100/2020, UIDP/00100/2020, LA/P/0056/2020, UID/Multi/04413/2020, CEECIND/03247/2018 and DL57/CEECIND/0256/2017. Publisher Copyright: Copyright © 2022 Marquês, Frazão De Faria, Reis, Machado, San...
Designing new antitubercular isoniazid derivatives with improved reactivity and...
de Faria, Catarina Frazão; Moreira, Tânia; Lopes, Pedro; Costa, Henrique; Krewall, Jessica R.; Barton, Callie M.; Santos, Susana; Goodwin, DouglasFunding Information: We acknowledge Diogo Vila Vi?osa for valuable discussions. We acknowledge financial support from Funda??o para a Ci?ncia e a Tecnologia, Portugal through projects PTDC/MED-QUI/29036/2017, PTDC/BIA-MIC-30692/2017, UIDB/00100/2020, UIDP/00100/2020, UIDB/04046/2020, UIDP/04046/2020, and UID/Multi/04413/2020, and Grants CEECIND/02300/2017 and DL57/CEECIND/0256/2017. Contributions from JRK, CMB,...
Electrochemical Detection of Single-Nucleotide Polymorphism Associated with Rif...
Ortiz, Mayreli; Jauset-Rubio, Miriam; Skouridou, Vasso; Machado, Diana; Viveiros, Miguel; Clark, Taane G.; Simonova, Anna; Kodr, David; Hocek, MichalFunding Information: This project received partial funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 767325, the Czech Science Foundation (20-00885X to M.H.), and the Fundação para a Ciência e a Tecnologia, Portugal (Grant - UID/Multi/04413/2020). The authors thank Fraunhofer ICT-IMM for the provision of gold electrode arrays. The Ph.D. scholarship of D. K...
Multicentre testing of the EUCAST broth microdilution reference method for MIC ...
Schön, Thomas; Werngren, Jim; Machado, Diana; Borroni, Emanuele; Wijkander, Maria; Lina, Gerard; Mouton, Johan; Matuschek, Erika; Kahlmeter, GunnarObjectives: The first objective of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) subcommittee for antimycobacterial susceptibility testing (AMST), launched in 2016, was to set a reference method for determining the MICs of antituberculous agents, since many protocols are used worldwide and a consensus one is needed for the determination of microbiological breakpoints. Methods: During 2...
Using genomics to understand the origin and dispersion of multidrug and extensi...
Perdigão, João; Gomes, Pedro; Miranda, Anabela; Maltez, Fernando; Machado, Diana; Silva, Carla; Phelan, Jody E.; Brum, Laura; Campino, SusanaPortugal is a low incidence country for tuberculosis (TB) disease. Now figuring among TB low incidence countries, it has since the 1990s reported multidrug resistant and extensively drug resistant (XDR) TB cases, driven predominantly by two strain-types: Lisboa3 and Q1. This study describes the largest characterization of the evolutionary trajectory of M/XDR-TB strains in Portugal, spanning a time-period of two...
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE n...
de Miranda, Silvana Spindola; de Almeida, Isabela Neves; de Fátima Filardi Oliveira Mansur, Maria; de Assis Figueredo, Lida Jouca(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. ...
Genome-wide analysis of Mycobacterium tuberculosis polymorphisms reveals lineag...
Oppong, Yaa E. A.; Phelan, Jody; Perdigao, Joao; Machado, Diana; Miranda, Anabela; Portugal, Isabel; Viveiros, Miguel; Clark, Taane G.Background: Continuing evolution of the Mycobacterium tuberculosis (Mtb) complex genomes associated with resistance to anti-tuberculosis drugs is threatening tuberculosis disease control efforts. Both multi- and extensively drug resistant Mtb (MDR and XDR, respectively) are increasing in prevalence, but the full set of Mtb genes involved are not known. There is a need for increased sensitivity of genome-wide ap...