31 documents found, page 1 of 4
Antimicrobial resistance in the food chain: bridging knowledge gaps for effecti...
Gomes, Emílio; Mesquita, Tomás Gonçalves; Serra, Patrícia; Araújo, Daniela; Almeida, Carina; Machado, António; Oliveira, Ricardo; Castro, JoanaAntimicrobial resistance (AMR) poses a critical global public health threat, with the food chain serving as a significant transmission route connecting animals, environment, and humans. This review adopts a One Health perspective to analyze the key drivers of AMR dissemination across animal agriculture, aquaculture and food processing. We evaluate detection methodologies, contrasting the regulatory gold standar...
On the Structure of Role Playing Game Quests
Machado, António; Santos, Pedro; Dias, JoãoIn this paper, we present a quest structure (in the form of a grammar) obtained from an analysis of the main quests of a single player role playing game (RPG), namely “The Witcher 3 - The Wild Hunt”. This grammar extends a previously presented analysis by other authors on MMORPG quests. This extended grammar is suitable for application in single player RPGs. The grammar allows the procedural generation of quest...
Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) fo...
Machado, António; Cerca, NunoFluorescence in situ hybridization (FISH) is a molecular method used to identify and quantify microorganisms in a wide range of samples. This technique combines the simplicity of microscopic observation and the specificity of DNA/rRNA hybridization, allowing detection of selected bacterial species and morphologic visualization. Here, we describe a quantitative molecular diagnosis of bacterial vaginosis, based o...
On the structure of role playing game quests
Machado, António; Santos, Pedro; Dias, JoãoIn this paper, we present a quest structure(in the form of a grammar)obtained from an analysis of the main quests of a single player role playing game (RPG), namely “The Witcher 3 -The Wild Hunt”. This grammar extends a previously presented analysis by other authors on MMORPG quests. This extended grammar is suitable for application in single player RPGs. The grammar allows the procedural generation of quests i...
Evidence for multiple founding lineages and genetic admixture in the evolution ...
Faria, Christiana; Machado, António; Amorim, Isabel R.; Gage, Matthew J. G.; Borges, Paulo A. V.; Emerson, Brent C.AIM: To infer colonization and speciation history for a closely related complex of nine species within the enigmatic Canary Island Laparocerus weevil radiation of 128 species. Using molecular dating and the spatial and temporal context that islands provide, we evaluate the possible explanations of incomplete lineage sorting and gene flow for the origin of shared genetic variation among species from different is...
Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluore...
Machado, António; Castro, J.; Cereija, Tatiana Barros Reis; Almeida, Carina; Cerca, NunoBacterial vaginosis (BV) is one of most common vaginal infections. However, its diagnosis by classical methods reveals low specificity. Our goal was to evaluate the accuracy diagnosis of 150 vaginal samples with research gold standard methods and our Peptide Nucleic Acid (PNA) probes by Fluorescence in situHybridization (FISH) methodology. Also, we described the first PNA-FISH methodology for BV diagnosis, whic...
The influence of biofilm formation by Gardnerella vaginalis and other anaerobes...
Machado, António; Cerca, NunoBacterial vaginosis (BV) is the worldwide leading vaginal disorder in women of reproductive age. BV is characterized by the replacement of beneficial lactobacilli and the augmentation of anaerobic bacteria. Gardnerella vaginalis is a predominant bacterial species, however, BV is also associated with other numerous anaerobes, such as Atopobium vaginae, Mobiluncus mulieris, Prevotella bivia, Fusobacterium nucleat...
Lactobacilli and its metabolites as potential probiotics against Gardenerella v...
Felgueiras, C.; Cereija, T.; Machado, António; Cerca, Nuno; Rodrigues, L. R.[Excerpt] Bacterial vaginosis (BV) is the most prevalent vaginal disorder in women of childbearing age, posing some significant health risks, such as abnormal pregnancy, pelvic inflammatory disease and an increased risk of sexual transmitted infections, including HIV [1]. It is characterized by a disruption in the normal balance of bacteria in the vagina with a reduction on lactobacilli number and an overgrowth...
Empiema Sub-Dural Causado por Salmonela. A Propósito dum Caso Clínico
João, Anabela; Godinho, Cristina; Rezende, Mário; Guedes, Margarida; Machado, AntónioInfecções intracraneanas focais constituem manifestações raras de salmonelose, podendo ocorrer como complicação de meningite, trauma ou hematoma intracraneano. Descreve-se o caso clínico de um empiema causado por Salmonela não typhy do grupo D, que complicou um hematoma sub-dural ocorrido num lactente de 8 meses, com boa evolução após terapêutica antibiótica e drenagem cirúrgica.
Gravidez não Vigiada — Que Risco Infeccioso no Recém-Nascido?
Madalena, Célia; Oliveira, Dulce; Guedes, Margarida; Machado, AntónioNo Sector de Lactentes do Serviço de Pediatria do Hospital Geral de Santo António tem-se assistido a um aumento do número de infecções congénitas diagnosticadas tardiamente. Por este motivo, os autores decidiram implementar um programa de rastreio a todos os Recém-Nascidos (RN) inetrnados com gestação não vigiada, visando avaliar a dimensão do risco infeccioso e as vantagens de instituir esse mesmo estudo a tod...