3 documents found, page 1 of 1
Clean-Room lithographical processes for the fabrication of Graphene biosensors
Cabral, Patrícia D.; Domingues, Telma; Machado, George; Chicharo, Alexandre; Cerqueira, M. F.; Fernandes, Elisabete; Athayde, Emilia; Alpuim, P.This work is on developing clean-room processes for the fabrication of electrolyte-gate graphene field-effect transistors at the wafer scale for biosensing applications. Our fabrication process overcomes two main issues: removing surface residues after graphene patterning and the dielectric passivation of metallic contacts. A graphene residue-free transfer process is achieved by using a pre-transfer, sacrificia...
Functionalization of single-layer graphene for immunoassays
Fernandes, Elisabete; Cabral, Patrícia D.; Campos, Rui; Machado, George; Cerqueira, M. F.; Sousa, Cláudia; Freitas, Paulo P.; Borme, JérômeThe excellent electronic properties of single-layer graphene provide the fundamental basis for its use in advanced electronics applications. Graphene-based biosensors, however, are one of the exceptions among the applications that take advantage of the unique properties of this material, because high hydrophobicity of graphene hinders the controlled immobilization of biomolecular probes, particularly of antibod...
Attomolar label-free detection of DNA hybridization with electrolyte-gated Grap...
Campos, Rui; Borme, Jérôme; Guerreiro, Joana Rafaela; Machado, George; Cerqueira, M. F.; Petrovykh, Dmitri Y.; Alpuim, P.In this work, we develop a field-effect transistor with a two-dimensional channel made of a single graphene layer to achieve label-free detection of DNA hybridization down to attomolar concentration, while being able to discriminate a single nucleotide polymorphism (SNP). The SNP-level target specificity is achieved by immobilization of probe DNA on the graphene surface through a pyrene-derivative heterobifunct...