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Exploring the effects of sweet potato leaves on skin health - from antimicrobia...

Machado, Manuela; Silva, Sara; Pintado, Manuela; Costa, Eduardo M.

Sweet potato leaves (SPL), an agricultural byproduct, hold significant potential in dermatological applications due to their bioactive compounds. This study evaluates the phenolic profile of SPL extracts and investigates their biological activities relevant to skin health. Extract fingerprinting, through uHPLC-DAD and LC–MS, identified phenolic acids and flavonoids, with cynarin, neochlorogenic acid, and spirae...


Modification of acorn starch structure and properties by high hydrostatic pressure

Castro, Luís M. G.; Caço, Ana I.; Pereira, Carla F.; Sousa, Sérgio C.; Brassesco, María E.; Machado, Manuela; Ramos, Óscar L.

Despite being rich in starch, over half of acorn production is undervalued. High hydrostatic pressure was used to modify the properties of Q. pyrenaica (0.1 and 460 MPa for 20 min) and Q. robur (0.1 and 333 MPa for 17.4 min) acorn starches to obtain high-valued ingredients. Pressure significantly altered the span distribution and heterogeneity of the acorn starch granules depending on the species, but their mor...


Application of encapsulated tomato by-product extract as a colorant in mascarpone

Molina, Adriana K.; Leichtweis, Maria G.; Machado, Manuela; Silva, Sara; Pintado, Manuela; Barreira, João C. M.; Dias, Maria Inês; Prieto, Miguel Á.

Agricultural by-products, often underutilized, represent a significant opportunity to obtain sustainable value-added products to develop new functional foods. In this context, the (typically discarded) aerial parts of tomato plants (Solanum lycopersicum L. var. cerasiforme), were studied as new sources of natural bioactive compounds, focusing the extraction and encapsulation (using spray drying with maltodextri...


Valorization of resistant starch from acorns as a new ingredient for chocolate ...

Castro, Luís M. G.; Sousa, Sérgio C.; Machado, Manuela; Alexandre, Elisabete M. C.; Saraiva, Jorge A.; Pintado, Manuela

Aim: Despite being a fruit rich in resistant starch, acorns remain undervalued. Resistant starch is known to improve food acceptability when compared to traditional insoluble fibers, and recent research suggests the usage of acorn starch as an additive in fermented yogurt and milk products. Furthermore, non-thermal technologies such as high hydrostatic pressure and pulsed electric field can produce clean-labell...


Soft gels in food systems: recent advances, applications, and technological inn...

Machado, Manuela; Costa, Eduardo Manuel Aguiar da; Silva, Sara

Soft gels, such as hydrogels, organogels, aerogels, and bigels, represent versatile materials that are increasingly utilized within food systems to modify texture, regulate nutrient delivery, serve as fat substitutes, and enhance product shelf life. Their structural diversity and tunable properties enable targeted solutions for healthier, more sustainable, and consumer-centric products. This review provides a c...


Bioactive food proteins: bridging nutritional and functional benefits with sust...

Machado, Manuela; Bautista-Hérnandez, Israel; Gómez-García, Ricardo; Silva, Sara; Costa, Eduardo M.

Bioactive food proteins play multifunctional roles in human health and functional food development. Beyond their nutritional value, these proteins contain peptide sequences capable of exerting physiological effects, such as antioxidant, anti-hypertensive, immunomodulatory, and anti-inflammatory activities. This review summarises the processing and functional technologies applied to bioactive proteins; the incre...


Biodegradable films with polysaccharides, proteins, and bioactive compounds fro...

Martins, Valter F. R.; Lopes, Ana I.; Machado, Manuela; Costa, Eduardo M.; Ribeiro, Tânia B.; Poças, Fátima; Pintado, Manuela; Morais, Rui M. S. C.

Microalgae are a sustainable source of bioactive compounds and nutrients that do not compete with crops for arable land. Lobosphaera sp. was used to produce biodegradable films. Bioactive compounds, polysaccharides, and proteins were extracted from this microalga. The total phenolic content (TPC) and antioxidant activity (ABTS, DPPH, and ORAC) of the bioactive-rich extract were determined, and its composition w...


Precision fermentation as a tool for sustainable cosmetic ingredient production

Silva, Sara; Bautista-Hérnandez, Israel; Gomez-García, Ricardo; Costa, Eduardo M.; Machado, Manuela

Precision fermentation, a highly controlled process of microbial fermentation, is emerging as a transformative tool to produce cosmetic ingredients. This technology leverages engineered micro-organisms to produce high-value compounds with applications in skincare, hair care, and other cosmetic formulations. Unlike traditional methods of ingredient sourcing, which often rely on extraction from plants or animals,...


From waste to wellness: sardine by-products as rich sources of bioactive ingred...

Machado, Manuela; Costa, Eduardo; Augusto, Helga; Cação, Ana; Pintado, Manuela E.

The sustainable utilization of sardine by-products represents a valuable contribution to addressing global challenges in waste management and health nutrition. Sardine byproducts, typically discarded or utilized as low-value inputs in animal feed and fertilizer, have demonstrated potential as a source of bioactive ingredients. This study highlights the composition and bioactive properties of sardine protein hyd...


Valorizing sardine by-products: sustainable functional canned tuna through prot...

Machado, Manuela; Cunha, Sara; Augusto, Helga; Cação, Ana; Pintado, Manuela

The development of novel functional foods is critical to addressing current consumer demands for health-promoting, sustainable products. This study explores the incorporation of sardine protein hydrolysates into the canned tuna sauce (tomato sauce), aiming to enhance its nutritional profile and functional properties while utilizing underexploited fishery by-products. Sardine protein hydrolysates were selected d...


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