81 documents found, page 1 of 9
Elastin-like recombinamers as a biotechnological platform for the development o...
Gonçalves, Anabela; Pinho, Vanessa; da Costa, André; Ribeiro, Artur; Gomes, Andreia C; Machado, RaulOncostatin M (OSM) and leukemia inhibitory factor (LIF) are pleiotropic cytokines from the interkeukine-6 family, associated with several disorders, and present significant potential in biomedicine. However, their therapeutic use is highly constrained by factors such as short circulating half-life and narrow therapeutic window. The conjugation of cytokines with elastin-like recombinamers (ELR) holds the potenti...
pH-responsive hybrid nanoassemblies for cancer treatment: formulation developme...
Teixeira, Patrícia V.; Adega, Filomena; Martins-Lopes, Paula; Machado, Raul; Lopes, Carla Martins; Lúcio, MarleneCurrent needs for increased drug delivery carrier efficacy and specificity in cancer necessitate the adoption of intelligent materials that respond to environmental stimuli. Therefore, we developed and optimized pH-triggered drug delivery nanoassemblies that exhibit an increased release of doxorubicin (DOX) in acidic conditions typical of cancer tissues and endosomal vesicles (pH 5.5) while exhibiting significa...
Valorisation of the invasive macroalgae Undaria pinnatifida (Harvey) Suringar f...
González-Ballesteros, Noelia; Fernandes, Mário; Machado, Raul; Sampaio, Paula; Gomes, Andreia C.; Cavazza, Antonella; Bigi, FrancaBacterial and fungal infections are a challenging global problem due to the reported increasing resistance of pathogenic microorganisms to conventional antimicrobials. Nanomaterials are a promising strategy to fight infections caused by multidrug-resistant microbes. In this work, gold (Au@UP) and silver (Ag@UP) nanoparticles were produced for the first time by green synthesis using an aqueous extract of the inv...
pH-responsive hybrid nanoassemblies for cancer treatment: formulation developme...
Teixeira, Patrícia V.; Adega, Filomena; Martins-Lopes, Paula; Machado, Raul; Lopes, Carla M.; Lúcio, M.Current needs for increased drug delivery carrier efficacy and specificity in cancer necessitate the adoption of intelligent materials that respond to environmental stimuli. Therefore, we developed and optimized pH-triggered drug delivery nanoassemblies that exhibit an increased release of doxorubicin (DOX) in acidic conditions typical of cancer tissues and endosomal vesicles (pH 5.5) while exhibiting significa...
Antimicrobial and anti-biofilm activity of silver nanoparticles biosynthesized ...
Fernandes, Mário; González-Ballesteros, Noelia; da Costa, André; Machado, Raul; Gomes, Andreia C; Rodríguez-Argüelles, Maria CarmenAntimicrobial resistance is an ever-growing global concern to public health with no clear or immediate solution. Silver nanoparticles (AgNPs) have long been proposed as efficient agents to fight the growing number of antibiotic-resistant strains. However, the synthesis of these particles is often linked to high costs and the use of toxic, hazardous chemicals, with environmental and health impact. In this study,...
Uncovering the promiscuous activity of IL-6 proteins: a multi-dimensional analy...
da Costa, André; Franco-Duarte, Ricardo; Machado, Raul; Gomes, Andreia CThe IL-6 family of cytokines, known for their pleiotropic behavior, share binding to the gp130 receptor for signal transduction with the necessity to bind other receptors. Leukemia inhibitory factor receptor is triggered by the IL-6 family proteins: leukemia inhibitory factor (LIF), oncostatin-M (OSM), cardiotrophin-1 (CT-1), ciliary neurotrophic factor (CNTF), and cardiotrophin-like cytokine factor 1 (CLCF1). ...
Repensar a universidade: desempenho acadêmico e comparações internacionais
Marcovitch, Jacques; Goldemberg, José; Axel-Berg, Justin; Righetti, Sabine; Santos, Solange Maria dos; Oliveira, Luiz Nunes deAlexis de Tocqueville, contemplando a tecnologia emergente na América, em meados do século 19, resumiu uma boa premissa de governança: “um novo mundo pede novas políticas”. Hoje, para usarmos uma referência bem próxima, o movimento de adequação das universidades estaduais paulistas às exigências dos novos tempos não poderia vir apenas de uma instituição, isoladamente. Foi unânime, destarte, a decisão de seu Con...
Omega-3- and resveratrol-loaded lipid nanosystems for potential use as topical ...
Caldas, Ana R.; Catita, José; Machado, Raul; Ribeiro, Artur; Cerqueira, Fátima; Horta, Bruno; Medeiros, Rui; Lúcio, Marlene; Lopes, Carla M.Resveratrol (RSV) and omega 3 (!3), because of their biological favorable properties, have become subjects of interest for researchers in dermocosmetic and pharmaceutical industries; however, these bioactives present technological limitations that hinder their effective delivery to the target skin layer. To overcome the stability and skin permeation limitations of free bioactives, this work proposes a combined ...
Protein-engineered polymers functionalized with antimicrobial peptides for the ...
Pereira, Ana Margarida Macedo Bernardes; Gomes, Diana Cristina Serra; da Costa, André; Dias, Simoni Campos; Casal, Margarida; Machado, RaulAntibacterial resistance is a major worldwide threat due to the increasing number of infections caused by antibiotic-resistant bacteria with medical devices being a major source of these infections. This suggests the need for new antimicrobial biomaterial designs able to withstand the increasing pressure of antimicrobial resistance. Recombinant protein polymers (rPPs) are an emerging class of nature-inspired bi...
Protein-based films functionalized with a truncated antimicrobial peptide seque...
da Costa, André; Pereira, Ana M.; Sampaio, Paula; Rodriguez-Cabello, Jose Carlos; Gomes, Andreia C; Casal, Margarida; Machado, RaulThe increasing bacterial resistance to antibiotics is driving strong demand for new antimicrobial biomaterials. This work describes the fabrication of free-standing films exhibiting antimicrobial properties by combining, in the same polypeptide chain, an elastin-like recombinamer comprising 200 repetitions of the pentamer VPAVG (A200) and an 18-amino-acid truncated variant of the antimicrobial peptide BMAP-28, ...