75 documents found, page 1 of 8
Follow-up of dairy cattle naturally infected by Trypanosoma vivax after treatme...
Castilho Neto,Kayo José Garcia de Almeida; Garcia,Ana Beatriz da Cruz Favaro; Fidelis Junior,Otavio Luiz; Nagata,Walter Beterquini; André,Marcos RogérioAbstract Trypanosoma vivax infections cause nonspecific clinical signs in cattle associated with aparasitemic intervals, making disease diagnosis a challenge. In Brazil, diminazene aceturate and isometamidium chloride (ISM) are available to treat bovine trypanosomosis. The objective of this study was to follow-up, by molecular and serological techniques, dairy cattle naturally infected by T. vivax after ISM tre...
Genomic analysis on Brazilian strains of Anaplasma marginale
Dall’Agnol,Bruno; Webster,Anelise; Souza,Ugo Araújo; Barbieri,Antonela; Mayer,Fabiana Quoos; Cardoso,Gisele Antoniazzi; Torres,Tatiana TeixeiraAbstract Anaplasma marginale is a vector-borne pathogen that causes a disease known as anaplasmosis. No sequenced genomes of Brazilian strains are yet available. The aim of this work was to compare whole genomes of Brazilian strains of A. marginale (Palmeira and Jaboticabal) with genomes of strains from other regions (USA and Australia strains). Genome sequencing of Brazilian strains was performed by means of n...
Analysis on the prokaryotic microbiome in females and embryonic cell cultures o...
Luzzi,Mayara de Cassia; Carvalho,Lucas Amoroso Lopes de; Pinheiro,Daniel Guariz; Lima-Duarte,Leidiane; Camargo,Jaqueline Valéria; Kishi,Luciano TakeshiAbstract Two lineages of Rhipicephalus sanguineus are known in Brazil: the temperate or southern and the tropical or northern populations. The distribution patterns of both lineages of R. sanguineus have epidemiological implications that can affect vectorial competence concerning Ehrlichia canis, the agent of canine monocytic ehrlichiosis. Intending to identify the microbiomes of both lineages and compare micro...
Genetic diversity of Hepatozoon spp. in rodents from Chile
Alabí,Amir Salvador; Monti,Gustavo; Otth,Carola; Sepulveda-García,Paulina; Perles,Livia; Machado,Rosangela Zacarias; André,Marcos RogérioAbstract This study aimed to investigate the genetic diversity of Hepatozoon spp. in rodents from Valdivia, Chile. A total of 74 rodents (synanthropic n=38; wild n=36) were trapped in Valdivia. We performed conventional PCR assays for Apicomplexa organisms targeting two overlapping 18S rDNA gene fragments (600 bp and 900 bp) followed by sequencing of selected amplicons. Hepatozoon spp. occurrence was 82.43% (61...
High level of infection by Toxoplasma gondii in pigs slaughtered in the city of...
Silva,Ellainy Maria Conceição; Sousa,Pablo dos Santos; Carvalho,Scarleth Kevia Gomes Silva de; Marques,Ingrid Carolinne Lopes; Costa,Francisco BorgesAbstract A serological, molecular and histopathological study was carried out in order to investigate occurrences of Toxoplasma gondii in pigs slaughtered with and without inspection service. Serum samples were collected from 60 pigs to detect anti-T. gondii antibody by indirect fluorescent antibody (IFAT). Tongue, masseter and diaphragm fragments were also collected for parasite DNA detection by means of the p...
Detection of Trypanosoma vivax in tissues of experimentally infected goats: wha...
Machado,Rosangela Zacarias; Castilho Neto,Kayo José Garcia de Almeida; Gonçalves,Luiz Ricardo; Machado,Gisele Fabrino; Luvizotto,Maria Cecília RuiAbstract Trypanosomiasis, caused by Trypanosoma vivax, is responsible for great economic losses among livestock in Africa and South America. During the life cycle of these parasites, they may present different morphological, metabolic and physiological characteristics depending on the interactions that are encountered at each point of their life cycle. Although T. vivax is frequently reported in the circulation...
High genetic diversity of Anaplasma marginale infecting dairy cattle in northea...
Pereira,José Gomes; Garcia,Amanda Barbosa; Gonçalves,Luiz Ricardo; Ramos,Inalda Angélica de Souza; Braga,Maria do Socorro Costa OliveiraAbstract Anaplasma marginale is an obligate intracellular Gram-negative bacterium found in ruminants’ erythrocytes and is the etiological agent of bovine anaplasmosis. The bacterium’s genetic diversity has been characterized based on sequences of major surface proteins (MSPs), such as MSP1α. The aim of the present study was to investigate the genetic diversity of A. marginale in cattle in the state of Maranhão,...
Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pa...
Ramos,Inalda Angélica de Souza; Mello,Victória Valente Califre de; Mendes,Natalia Serra; Zanatto,Diego Carlos de Souza; Campos,João Bosco VilelaAbstract This study investigated the seropositivity for five different tick-borne agents, namely Anaplasma marginale, Babesia bovis, Babesia bigemina, Coxiella burnetii, Anaplasma phagocytophilum, and Trypanosoma vivax in beef cattle in the Brazilian Pantanal. The serum samples collected from animals (200 cows; 200 calves) were used in indirect enzyme-linked immunosorbent assays (iELISA) to detect IgG antibodie...
Genetic diversity of Babesia bovis studied longitudinally under natural transmi...
Matos,Carlos António; Silva,Jenevaldo Barbosa da; Gonçalves,Luiz Ricardo; Mendes,Natalia Serra; Alvarez,Dasiel Obregón; André,Marcos RogérioAbstract Serum and DNA samples from 15 naturally infected calves in Seropédica, Brazil, were obtained quarterly from birth to 12 months of age, in order to longitudinally evaluate their humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis. Anti-B. bovis IgG antibodies were detected by an indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent as...
Detection of a putative novel genotype of Ehrlichia sp. from opossums (Didelphi...
Guimarães,Andresa; Raimundo,Juliana Macedo; Silva,Aline Tonussi da; Carpintero,Fernanda Modesto; Pires,Jeferson Rocha; Benevenute,Jyan LucasAbstract Erlichiosis affects humans and animals worldwide. Its distribution and prevalence depends on the presence of tick vectors and hosts in one geographic area. The aim of the present study was to investigate the occurrence of Ehrlichia spp. and Anaplasma spp. in opossums (Didelphis sp.) from the State of Rio de Janeiro, southeast Brazil. Blood samples from 37 animals were tested for these two pathogens usi...