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Evaluation of the Chagas Disease Control Program in Açucena Municipality, Rio D...
Santos,Adriana dos; Letro,Rejane Balmant; Lemos do Bem,Vitor Antônio; Azeredo,Bernardino Vaz de Melo; Coelho,George Luiz Lins Machado; Diotaiuti,LiléiaIntroduction Açucena Municipality, Rio Doce Valley, State of Minas Gerais, Brazil temporarily (2001-2005) interrupted epidemiological surveillance for Chagas disease. The objective of this work was to evaluate the Chagas Disease Control Program (CDCP) in Açucena and to offer suggestions for improving local epidemiological surveillance. Methods This study was conducted in three phases: I) a serological investiga...
In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for diff...
Santos,Lilian da Silva; Torres,Rosália Morais; Machado-de-Assis,Girley Francisco; Bahia,Maria Terezinha; Martins,Helen RodriguesINTRODUCTION: The goal was to develop an in-house serological method with high specificity and sensitivity for diagnosis and monitoring of Chagas disease morbidity. METHODS: With this purpose, the reactivities of anti-T. cruzi IgG and subclasses were tested in successive serum dilutions of patients from Berilo municipality, Jequitinhonha Valley, Minas Gerais, Brazil. The performance of the in-house ELISA was al...