17 documents found, page 1 of 2

Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vul...

Lactic acid bacteria (LAB) have been documented as potential vitamin B12 producers and may constitute an exogenous source of cobalamin for the microalga Chlorella vulgaris, which has been described as being able to perform vitamin uptake. Hence, there is an interest in discovering novel B12-producing probiotic LAB. Therefore, the purpose of the current work was to perform a phenotype–genotype analysis of the vi...

Development of a novel phagomagnetic-assisted isothermal DNA amplification syst...

The hitherto implemented Listeria monocytogenes detection techniques are cumbersome or require expensive non-portable instrumentation, hindering their transposition into on-time surveillance systems. The current work proposes a novel integrated system resorting to loop-mediated isothermal amplification (LAMP), assisted by a bacteriophage P100–magnetic platform, coupled to an endpoint electrochemical technique, ...

The impact of HPP-assisted biocontrol approach on the bacterial communities’ dy...

Traditional foods are increasingly valued by consumers, whose attention and purchase willingness are highly influenced by other claims such as ‘natural’, ‘sustainable’, and ‘clean label’. The purpose of the present study was to evaluate the impact of a novel non-thermal food processing method (i.e., HPP-assisted biocontrol combining mild high hydrostatic pressure, listeriophage Listex, and pediocin PA-1 produci...

Electrostatic assembly of anti-listeria bacteriophages on a self-assembled mono...

The integration of bacteriophages, a particular class of viruses that specifically infect bacteria and archaea, in biosensors for the monitoring of pathogens in foods and beverages is highly desirable. To this end, an increasing focus has been set on the exploration of covalent and physical methods for the immobilization of phages on solid surfaces. This work investigates the electrostatic assembly of tailed ph...

Exploiting Potential Probiotic Lactic Acid Bacteria Isolated from Chlorella vul...

Lactic acid bacteria (LAB) have been documented as potential vitamin B12 producers and may constitute an exogenous source of cobalamin for the microalga Chlorella vulgaris, which has been described as being able to perform vitamin uptake. Hence, there is an interest in discovering novel B12-producing probiotic LAB. Therefore, the purpose of the current work was to perform a phenotype–genotype analysis of the vi...

High-pressure processing and lytic bacteriophage cocktail phageguard S as a syn...

Salmonella spp. still represents a major public health concern as the etiological agent of foodborne diarrhoeal illnesses. Salmonella Enteritidis is the most common serovar associated with non-typhoidal salmonellosis in the United States and Europe, through the ingestion of raw, undercooked eggs or egg-derived food products. The consumption pattern has changed worldwide, towards a growing demand for minimally p...

Impact of high hydrostatic pressure on the stability of lytic bacteriophages co...

This work consisted in the first comprehensive study in which the potential to exploit the Salmonella lytic bacteriophages cocktail, Salmonelex™, in association with high hydrostatic pressure (HHP) towards potential application in egg matrices decontamination was evaluated. The impact of HHP (200–600 MPa) on the bacteriophages' viability pointed out a stability in the range of 200–400 MPa. From 400 MPa onwards,...

Innovative hurdle system towards Listeria monocytogenes inactivation in a ferme...

Consumers' quest for healthier, locally produced foods, renders the demand for these products increasingly prominent. The purpose of the present work was to evaluate the impact of a non-thermal multi-hurdle approach, which associated mild high hydrostatic pressure (HHP, 300 MPa), the bacteriophage Listex™ P100, and the pediocin PA-1 producing Pediococcus acidilactici HA 6111-2, as a novel minimal processing tow...

Non-thermal approach to Listeria monocytogenes inactivation in milk: the combin...

Non-thermal food processing and replacement of chemical additives by natural antimicrobials are promising trends in the food industry. The objective of the present work was to evaluate the effect of a process which combines mild high hydrostatic pressure – HHP (200 and 300 MPa, 5 min, 10 °C), phage Listex™ P100 and the bacteriocin pediocin PA-1 as a new non-thermal process for destruction of Listeria monocytoge...

Development of a flow-based system for the determination of protein content in ...