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Insights on microalgae-based technologies with potential impact on global metha...

Madureira, Leandro Filipe Feiteira; Soares, Ana Sofia Maciel; Duarte, Maria Salomé Lira; Pereira, Francisco; Francisco, Diogo

The European Unions Methane Action Plan outlined policies and targets supporting the Global Methane Pledge to cut CH4 emissions by 30% by 2030, yet urgent implementation is needed to treat medium and diluted CH4 streams. Microalgae-based technologies offer a groundbreaking solution, merging CH4 mitigation with biomass valorization to drive sustainable industrial practices. This review examines three key applica...


Electric fields to support microalgae growth with a differentiated biochemical ...

Barreiros, Mariana Pereira; Maciel, José Filipe Gonçalves; Pereira, Ricardo S.; Vicente, A. A.; Pereira, Ricardo Nuno Correia

Microalgae are a group of microorganisms well-known for their high metabolic plasticity and biomass with commercial interest for several industries. In the present work, the influence of in-situ electric field application (INEF) on the growth and biochemical composition of Pavlova gyrans was, for the first time, assessed. Electrical protocols were tested, namely by applying INEF in different growth stages, in v...


Development of highly effective growth strategies aiming at improving the conte...

Sousa, Vítor Emanuel Silva; Maciel, José Filipe Gonçalves; Vicente, A. A.; Dias, Oscar; Geada, Pedro Miguel Macedo

Dunaliella salina is the most promising natural source of b-carotene, presenting itself as a valid alternative to traditional chemically synthesized carotenoids. Microalgal pigments present several advantages compared to their synthetically produced counterparts, revealing, for instance, higher bioaccessibility. In the present study, a central composite rotatable design and a central composite design were emplo...


Life cycle assessment of lipid production from Pavlova gyrans: Influence of the...

Novais, Roberto Daniel Machado; Mata, Teresa M.; Madureira, Leandro Filipe Feiteira; Maciel, José Filipe Gonçalves; Vicente, A. A.; Martins, António A.

Microalgae have been increasingly recognized as an alternative source of lipids, especially polyunsaturated fatty acids (PUFA) such as omega 3 and 6, being essential to carefully assess their life cycle environmental impacts. Hence, this work aims to carry out a life cycle assessment (LCA) of lipid production from the microalga Pavlova gyrans grown in different culture media and nutrient concentrations, on a cr...


The potential of Pavlovophyceae species as a source of valuable carotenoids and...

Maciel, José Filipe Gonçalves; Madureira, Leandro Filipe Feiteira; Geada, Pedro Miguel Macedo; Teixeira, J. A.; Silva, Joana; Vicente, A. A.

Microalgae are a group of microorganisms, mostly photoautotrophs with high CO2 fixation capacity, that have gained increased attention in the last decades due to their ability to produce a wide range of valuable metabolites, such as carotenoids and polyunsaturated fatty acids, for application in food/feed, pharmaceutical, and cosmeceutical industries. Their increasing relevance has highlighted the importance of...


Identification and optimization of the key growth parameters involved in carote...

Maciel, José Filipe Gonçalves; Berni, Paulo; Geada, Pedro Miguel Macedo; Teixeira, J. A.; Silva, Joana; Vicente, A. A.

In this work, a multivariate analysis was carried out, using a PlackettBurman (PB) design involving seventeen growth parameters, on carotenoids production of Pavlova gyrans (p<0.10). Each assay was analysed regarding its content (mg g1) of fucoxanthin (Fx), diatoxanthin, diadinoxanthin, -carotene (Car), -carotene, and the sum of all carotenoids analysed individually (TCar). According to the statistical analysis...


Exploring methane mitigation strategies in photosynthetic microorganisms throug...

Apolinário, Gonçalo; Gonçalves, Joana Oliveira; Cunha, Emanuel Rodrigues; Madureira, Leandro Filipe Feiteira; Maciel, José Filipe Gonçalves

The problematic of greenhouse gas (GHG) emissions is a global environmental challenge that has raised concerns in the past few decades. Particularly, the increase in atmospheric concentrations of carbon dioxide, methane, and other damaging gases can lead to catastrophic repercussions to life as we know it. Therefore, reducing GHG emissions and fomenting strategies for their mitigation are crucial steps that nee...


Effect of ohmic heating on the extraction of biocompounds from aqueous and etha...

Maciel, José Filipe Gonçalves; Machado, Luís Pedro Lima; Silva, Joana; Pereira, Ricardo Nuno Correia; Vicente, A. A.

Electric field technology has been highlighted as a promising strategy in the permeabilization of cell membranes and the corresponding bioactive release. In this work, aqueous and ethanolic suspensions of Pavlova gyrans were subjected to moderate electric fields (MEF) to promote rapid heating due to Ohmic Heating (OH) effect. Two approaches were tested: i) OH assisted extraction in a temperature range between 2...


Optimization of Pavlova gyrans biomass production and the fatty acid profile us...

Maciel, José Filipe Gonçalves; Couto, Daniela; Geada, Pedro Miguel Macedo; Pereira, Hugo; Teixeira, J. A.; Domingues, M. Rosário; Silva, Joana

This work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO4·5H2O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4·H2O, these variables were further studied in order to maximize the biomass production of P. gyra...


Microalgae production strategies to increase carotenoid and polyunsaturated fat...

Maciel, José Filipe Gonçalves

As microalgas têm sido destacadas como uma fonte promissora e sustentável de importantes compostos bioactivos para as indústrias farmacêutica e alimentar, como por exemplo: carotenóides, ácido eicosapentaenóico (EPA) e ácido docosahexaenóico (DHA). Este trabalho teve como objetivo otimizar e caracterizar a produção de biomassa da Pavlova gyrans através de uma abordagem multivariada. Inicialmente foram avaliados...


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