The European Unions Methane Action Plan outlined policies and targets supporting the Global Methane Pledge to cut CH4 emissions by 30% by 2030, yet urgent implementation is needed to treat medium and diluted CH4 streams. Microalgae-based technologies offer a groundbreaking solution, merging CH4 mitigation with biomass valorization to drive sustainable industrial practices. This review examines three key applica...
Microalgae are a group of microorganisms well-known for their high metabolic plasticity and biomass with commercial interest for several industries. In the present work, the influence of in-situ electric field application (INEF) on the growth and biochemical composition of Pavlova gyrans was, for the first time, assessed. Electrical protocols were tested, namely by applying INEF in different growth stages, in v...
Dunaliella salina is the most promising natural source of b-carotene, presenting itself as a valid alternative to traditional chemically synthesized carotenoids. Microalgal pigments present several advantages compared to their synthetically produced counterparts, revealing, for instance, higher bioaccessibility. In the present study, a central composite rotatable design and a central composite design were emplo...
Microalgae have been increasingly recognized as an alternative source of lipids, especially polyunsaturated fatty acids (PUFA) such as omega 3 and 6, being essential to carefully assess their life cycle environmental impacts. Hence, this work aims to carry out a life cycle assessment (LCA) of lipid production from the microalga Pavlova gyrans grown in different culture media and nutrient concentrations, on a cr...
Microalgae are a group of microorganisms, mostly photoautotrophs with high CO2 fixation capacity, that have gained increased attention in the last decades due to their ability to produce a wide range of valuable metabolites, such as carotenoids and polyunsaturated fatty acids, for application in food/feed, pharmaceutical, and cosmeceutical industries. Their increasing relevance has highlighted the importance of...
In this work, a multivariate analysis was carried out, using a PlackettBurman (PB) design involving seventeen growth parameters, on carotenoids production of Pavlova gyrans (p<0.10). Each assay was analysed regarding its content (mg g1) of fucoxanthin (Fx), diatoxanthin, diadinoxanthin, -carotene (Car), -carotene, and the sum of all carotenoids analysed individually (TCar). According to the statistical analysis...
The problematic of greenhouse gas (GHG) emissions is a global environmental challenge that has raised concerns in the past few decades. Particularly, the increase in atmospheric concentrations of carbon dioxide, methane, and other damaging gases can lead to catastrophic repercussions to life as we know it. Therefore, reducing GHG emissions and fomenting strategies for their mitigation are crucial steps that nee...
Electric field technology has been highlighted as a promising strategy in the permeabilization of cell membranes and the corresponding bioactive release. In this work, aqueous and ethanolic suspensions of Pavlova gyrans were subjected to moderate electric fields (MEF) to promote rapid heating due to Ohmic Heating (OH) effect. Two approaches were tested: i) OH assisted extraction in a temperature range between 2...
This work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO4·5H2O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4·H2O, these variables were further studied in order to maximize the biomass production of P. gyra...
As microalgas têm sido destacadas como uma fonte promissora e sustentável de importantes compostos bioactivos para as indústrias farmacêutica e alimentar, como por exemplo: carotenóides, ácido eicosapentaenóico (EPA) e ácido docosahexaenóico (DHA). Este trabalho teve como objetivo otimizar e caracterizar a produção de biomassa da Pavlova gyrans através de uma abordagem multivariada. Inicialmente foram avaliados...