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Estimating the biodegradation of PHB/PBAT films - An experimental design approach

Fernandes, Miguel ângelo Cavaleiro; Salvador, Andreia Filipa Ferreira; Madalena, Daniel Alexandre Silva; Vicente, A. A.

The massive use of plastics in various applications, particularly packaging, generates enormous amounts of plastic waste that can be found almost everywhere, including in soil. This represents a serious environmental pollution issue since the biodegradability in soils can take several years, depending on the microbial composition, the physical and chemical characteristics of plastics, and other relevant environ...


Development of 3D-printed foods incorporating riboflavin-loaded whey protein is...

Araújo, João Miguel Fernandes; Fernandes, Jean Michel; Madalena, Daniel Alexandre Silva; Gonçalves, Raquel Filipa Silva; Vieira, Jorge Miguel Magalhães

3D printing has emerged as a groundbreaking technology, aiming to enhance sensory attributes and improving nutritional/functional aspects. Simultaneously, nano-delivery systems have emerged as an opportunity to protect bioactive compounds against degradation and improve their bioaccessibility. Therefore, a novel concept is underway, involving the 3D printing of perishable healthy foods previously fortified with...


Co-encapsulation of vitamin D3 and curcumin in plant protein-based nanoemulsion...

Marques, Márcia; Gonçalves, Raquel Filipa Silva; Madalena, Daniel Alexandre Silva; Abrunhosa, Luís; Vicente, A. A.; Pinheiro, A. C.

This study aimed to develop plant-based nanoemulsions (NE) for co-encapsulation of vitamin D3 and curcumin, comparing the emulsifying properties of pea and potato proteins. Different oil and protein concentrations were tested, and formulations with the smallest particle size were characterized and submitted to harmonized static in vitro digestion. Formulations prepared with the highest protein concentration (10...


Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using th...

Madalena, Daniel Alexandre Silva; Araújo, João Miguel Fernandes; Ramos, Óscar Leandro Silva; Vicente, A. A.; Pinheiro, A. C.

Nanosized delivery systems have been the subject of research and discussion in the scientific community due to their unique properties and functionality. However, studies reporting the behaviour of nanodelivery systems under dynamic in vitro digestion conditions are still very scarce. To address this gap, this study aims to assess the dynamic in vitro gastric digestion of lactoferrin/curcumin nanoparticles in t...


Design of a 3D in vitro gastric model with in situ analysis for the assessment ...

Madalena, Daniel Alexandre Silva

The regular consumer is becoming more concern regarding their nutritional habits and, as such, novel healthier food products with additional functional properties must be developed to give response to the increase from the food market demand. However, while developing innovative food products through, for instance, the addition of nanosystems for the controlled delivery of bioactive compounds, it is important t...


Emerging challenges in assessing bio-based nanosystems’ behaviour under in vitr...

Madalena, Daniel Alexandre Silva; Fernandes, Jean Michel; Avelar, Zita Sofia Barbosa; Gonçalves, Raquel Filipa Silva; Ramos, Óscar L.; Vicente, A. A.

The current consumers demand for high quality food products together with the growing awareness regarding the link between health and nutrition has led to the development of novel food products with added functionality. Such functionality can be modulated by adding bio-based nanosystems that can improve the bioaccessibility of bioactive compounds and facilitate nutrient absorption. However, these functional pro...


Modelo dinâmico de digestão gástrica e programa de computador

Pinheiro, A. C.; Madalena, Daniel Alexandre Silva; Vicente, A. A.; Ramos, Óscar Leandro Silva

A presente invenção diz respeito a um modelo gástrico modular in vitro com natureza dinâmica e análise in situ, para monitorização em tempo real do processo de digestão de alimentos, sistemas de libertação controlada, compostos bioativos e fármacos. Este modelo compreende pelo menos 7 módulos capazes de replicar as condições físico-químicas que ocorrem ao longo da digestão no estômago humano. A entrada do estôm...


Valorization of Amaranth (Amaranthus cruentus) grain extracts for the developme...

Coelho, Laylla Marques; Faria, C. I.; Madalena, Daniel Alexandre Silva; Genisheva, Zlatina Asenova; Martins, Joana T.; Vicente, A. A.; Pinheiro, A. C.

This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents. Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films. An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film...


Development of a realistic in vitro digestion model (RGM) coupled UV-VIS-SWNIR ...

Madalena, Daniel Alexandre Silva; Pinheiro, A. C.; Ramos, Oscar; Vicente, A. A.

Background: The development of realistic gastric models unlocked the possibility of studying important digestion phenomena occurring during the digestion of food (e.g., retropulsion). Understanding the dynamics of food digestion in real-time, without sample manipulation, is still a challenge, but brings a huge potential in providing important insights regarding the dynamic process of food digestion (e.g., real ...


Application of nanostructured delivery systems in food: From incorporation to d...

Gonçalves, Raquel Filipa Silva; Madalena, Daniel Alexandre Silva; Fernandes, Jean Michel; Marques, Márcia Cristina Teixeira; Vicente, A. A.

Background The development of nanostructures for food applications is growing exponentially, due to their potential to improve foods' nutritional and health value as well as foods' shelf-life. However, there is still a lack of information regarding the nanostructures' effects on the complex food matrices and vice versa, leading to some concerns about nanostructures safety and food quality. One of the reasons is...


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