This work reports the results of analyses of three complete mycoplasma genomes, a pathogenic (7448) and a nonpathogenic (J) strain of the swine pathogen Mycoplasma hyopneumoniae and a strain of the avian pathogen Mycoplasma synoviae; the genome sizes of the three strains were 920,079 bp, 897,405 bp, and 799,476 bp, respectively. These genomes were compared with other sequenced mycoplasma genomes reported in the...
Chromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spre...
The aim of this study was to evaluate functionally the effect of plumbagin on the heterologous expression of a predicted promoter region of open reading frames of paraquat-inducible (pqi) genes revealed during genome annotation analyses of the bacterium Chromobacterium violaceum. First, the promoter of interest was amplified using specific primers and cloned into a conjugative vector carrying the Escherichia co...
The aim of this work was to evaluate the agronomical characteristics, chemical composition and removal of potassium of five different genotypes of corn grown for silage. A split-plot complete randomized block design was used, with three hybrids (DKB 390, AGX 8517, A-2560) and two varieties (ALBianco, Piratininga), using two cut heights (20 and 40 cm above soil level) and four replications per treatment. After h...
The aim of this work was evaluating the agronomical and chemical characteristics of 20 different genotypes of maize used as silage. A complete randomized experimental design was used, with three replicates per treatment. Each hybrid was harvested when plants reached 1/3 of milk line stage, using two cut heights: immediately above the senescent leaves (whole-plant) and immediately below ear attachment (half-plan...
PCR analysis is a sensitive and specific tool to detect and monitor microorganisms in complex environmental samples. The amplification of 16S ribosomal DNA sequences followed by gel electrophoresis under denaturing gradient (DGGE) has been a powerful technique to genetically evaluate microbial ecosystems. Changes in rumen microbial populations were investigated in vitro using a basal diet with different lipid s...