22 documents found, page 1 of 3

Cryptic diversity in southern African kelp

The southern coast of Africa is one of the few places in the world where water temperatures are predicted to cool in the future. This endemism-rich coastline is home to two sister species of kelps of the genus Ecklonia maxima and Ecklonia radiata, each associated with specific thermal niches, and occuring primarily on opposite sides of the southern tip of Africa. Historical distribution records indicate that E....

Adjuvant Hyperthermic Intravesical Chemotherapy in Intermediate- and High-Risk ...

Introduction: Non-muscle invasive bladder cancer (NMIBC) is a frequently diagnosed neoplasm, which is typically managed with transurethral resection of bladder tumor (TURBT) eventually followed by intravesical therapies. Bacillus Calmette-Guérin (BCG) is used as first-line adjuvant treatment in high- (HR) and intermediate-risk (IR) NMIBC, although, in the latter, mitomycin C (MMC) may also be used. Multiple lim...

The Automatic Assessment of Strength and Mobility in Older Adults: A Test-Retes...

Abstract: Background: Simple field tests such as the Timed Up and Go test (TUG) and 30 s Chair Stand test are commonly used to evaluate physical function in the elderly, providing crude outcome measures. Using an automatic chronometer, it is possible to obtain additional kinematic parameters that may lead to obtaining extra information and drawing further conclusions. However, there is a lack of studies that ev...

Effect of osmolytes on the activity of anti-cancer enzyme L-Asparaginase II fro...

L-asparaginase is used for the treatment of acute lymphoblastic leukaemia (ALL); however, its formulation presents drawbacks such as a lack of stability and formation of aggregates. Osmolytes are small molecules accumulated by cells in response to environmental stresses and present a protective behaviour, favouring the equilibrium of macromolecules towards the native conformation. Therefore, osmolytes are emplo...

Manipulation of partition coefficients in ATPSs: understanding the addition of ...

[Excerpt] Efficient and economical downstream processing of biological products has been one of the main challenges of biotechnology industry. However, downstream methodologies have developed slowly and so new, fast and robust separation methods are urgently needed. Aqueous two-phase systems (ATPSs) are long known to be a promising separation technique and a valuable alternative to conventional chromatography. ...

Analysis of partitioning of organic compounds and proteins in aqueous polyethyl...

Partition behavior of nine small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.5 M osmolyte (sorbitol, sucrose, trehalose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polar...

Cancro Ginecológico – consensos nacionais 2013

O culpa é dos narradores: os contos fantásticos de Edgar Allan Poe

Esta tese consiste na análise de alguns contos de Edgar Allan Poe. Nela tento mostrar que os acontecimentos sobrenaturais estão subordinados aos interesses particulares dos narradores culpados de Poe e que, por essa razão, não merecem que o leitor lhes dê crédito. O centro do argumento é uma leitura literal de “Ligeia”. Analiso também o modo como a analogia entre quarto e mente determina a decoração de interior...

Correlations between distribution coefficients of various biomolecules in diffe...

Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)–polyethylene glycol (PEG) and Dex–Ucon 50-HB-5100 (Ucon—a random copolymer of ethylene glycol and propylene glycol) both containing 0.15MNaCl in 0.01Mphosphate buffer, pH 7.4, at 23 ◦C. Distri...

Relative hydrophobicity of (PEG or Ucon)-salt ATPSs

Aqueous Two-Phase Systems (ATPSs) are biphasic systems composed mainly by water. ATPSs are obtained upon mixing of two aqueous solutions of certain polymers or a polymer and a salt (above certain critical conditions, e.g. concentration, temperature). These systems are commonly indicated for the extraction of biomolecules. In this work, the partition coefficients for a series of five dinitrophenylated amino-acid...