87 documents found, page 1 of 9
Silk fibroin microparticles as a candidate for wound healing applications: eval...
Bernardes, Beatriz G.; Laurano, Rossella; López-Iglesias, Clara; Magalhães, Rui; Costa, Raquel; García-González, Carlos A.; Oliveira, Ana LeiteIn this study, silk fibroin (SF) aerogel microparticles were developed as a delivery system for adenosine (ADO) to exploit its anti-inflammatory and regenerative properties for wound healing. Supercritical CO2 drying was used to prepare ADO-loaded SF aerogel microparticles with different SF concentrations (3 %, 5 %, 7 % w/v) and SF: ADO ratios (10:1, 5:1, 2:1). Characterization revealed high porosity (91–94 %),...
Growth, oxidative stress, and intermediary metabolism of European seabass (Dice...
Filipe, Diogo; Magalhães, Rui; Fernandes, Helena; Salgado, José Manuel Seara; Belo, Isabel; Oliva-Teles, Aires; Peres, HelenaPrevious research demonstrated that solid-state fermentation (SSF) of distiller's dried grains with solubles (DDGS) by Aspergillus ibericus improved protein, reduced neutral and acid detergent fiber levels, and added phenolic compounds, xylanase, and cellulase. Fermentation of DDGS also increased protein, lipids, energy, and starch digestibility. This study evaluated the effects of unfermented and fermented DDG...
Effects of solid-state fermentation of Gelidium corneum by-product on immune st...
Ferreira, Marta Alexandra Lages; Ramos-Oliveira, Catarina; Magalhães, Rui; Martins, Nicole; Serra, Cláudia R.; Salgado, José Manuel Seara; Belo, IsabelIt was previously observed that dietary inclusion of 10 % Gelidium corneum by-product and sunflower cake mixture (1:1 ratio; Gmix), replacing corn gluten meal, reduced feed utilization efficiency in European sea bass juveniles, while the same mixture fermented with Aspergillus ibericus restored feed efficiency as well as nitrogen and energy utilization, achieving levels comparable to the control diet. The prese...
Expanding the potential of self-assembled silk fibroin as aerogel particles for...
Bernardes, Beatriz G.; Baptista-Silva, Sara; Illanes-Bordomás, Carlos; Magalhães, Rui; Dias, Juliana Rosa; Alves, Nuno M. F.; Costa, RaquelA newly produced silk fibroin (SF) aerogel particulate system using a supercritical carbon dioxide (scCO2)-assisted drying technology is herein proposed for biomedical applications. Different concentrations of silk fibroin (3%, 5%, and 7% (w/v)) were explored to investigate the potential of this technology to produce size- and porosity-controlled particles. Laser diffraction, helium pycnometry, nitrogen adsorpt...
The resilience of listeria monocytogenes to environmental stressors
Gonçalves, Catarina; Ferreira, Vânia; Magalhães, Rui; Teixeira, PaulaIntroduction: listeria monocytogenes is a significant foodborne pathogen capable of causing severe listeriosis, particularly in vulnerable people [1]. To prevent its spread, effective disinfection strategies are essential. Quaternary ammonium compounds, like benzalkonium chloride, and peracetic acid are commonly used biocides in food processing environments. However, L. monocytogenes can exhibit remarkable resi...
In the quest for reliable tools to explore the virulence potential of Listeria ...
Sousa, Mariana; Magalhães, Rui; Ferreira, Vânia; Teixeira, PaulaIntroduction and objective: Listeria monocytogenes is a foodborne pathogen that causes human listeriosis. This pathogen is characterized by an intra-species heterogeneity so its strains can be grouped into clonal complexes (CCs) that are defined either as hypervirulent – mainly associated with clinical cases or hypovirulent – associated with food or food processing environments and with infections in susceptibl...
Unraveling listeria monocytogenes resilience: does it survive on metallic surfa...
Gonçalves, Catarina; Ferreira, Vânia; Magalhães, Rui; Teixeira, PaulaListeria monocytogenes is a Gram-positive pathogenic bacterium that can cause listeriosis in humans [1,2]. A primary cause of human listeriosis is the consumption of food contaminated with L. monocytogenes. This rare yet severe foodborne illness results in high rates of hospitalization and mortality, particularly in vulnerable groups: the elderly, immunocompromised individuals, and pregnant women [3,4,5]. It ex...
Comparing growth and survival kinetics of persistent and sporadic strains of li...
Meneses, Rui; Pereira, Catarina; Ferreira, Vânia; Magalhães, Rui; Barbosa, Joana; Silva, Beatriz Nunes; Teixeira, PaulaMotivation: L. monocytogenes is known to persist in food processing environments, with some strains being routinely isolated (persistent), while others are only occasionally found (sporadic). This raises the question: Are persistent strains more resistant to stress conditions compared to sporadic strains from similar sources? Objective: To investigate the effect of food processing related stresses (pH=6 and 8% ...
Assessing the persister state in Listeria monocytogenes in response to gentamic...
Sousa, Mariana; Azevedo, Mónica; Magalhães, Rui; Almeida, Gonçalo; Teixeira, PaulaIntroduction: In the context of food safety, persister cells are a public health concern since these may contribute to the persistence and survival of bacteria in food processing environments. Listeria monocytogenes persisters are described as a subpopulation of growth-arrested cells that possess non-heritable, temporary tolerance to lethal doses of antibiotics (e.g., gentamicin) and can revert to an active sta...
Comparative analysis of fatty acid profiles in listeria monocytogenes isolates ...
Magalhães, Rui; Machado, Manuela; Teixeira, PaulaIntroduction: Listeria monocytogenes, the causative agent of listeriosis, is a major foodborne pathogen. It is a ubiquitous, facultative anaerobic, gram-positive bacterium. Listeriosis can be a severe illness particularly affecting vulnerable groups: the elderly, immunocompromised individuals, pregnant women and neonates. Listeria monocytogenes exhibits remarkable adaptability to diverse and challenging environ...