28 documents found, page 1 of 3
Fluoroquinolones: a preliminary study on optimized extraction and chromatograph...
Carvalho, Ana Rita; Vidal, Renata; Gonçalves, Virgínia M. F.; Maia, Alexandra S.; L. Ribeiro, Ana Rita; Ribeiro, CláudiaBackground: Fluoroquinolones (FQs) are a widely prescribed class of antibiotics, used in both human and veterinary medicine, commonly administered for prophylactic and therapeutic purposes [1]. Due to their extensive use and excretion in both unmetabolized form and metabolites, FQs and their metabolites persist in aquatic environments. As a result, these compounds are frequently detected in surface and groundwa...
Microbiome adaptation of aerobic granular sludge allows process resilience duri...
Miranda, Catarina; Maia, Alexandra S.; Tiritan, Maria Elizabeth; Castro, Paula M. L.; Amorim, Catarina L.Coastal wastewater treatment plants often face multiple stressors (e.g., pharmaceuticals and oscillating seawater levels) simultaneously, and their combined effects on biological treatment systems are still largely underestimated. In this study, an aerobic granular sludge (AGS) reactor was challenged over a four-month period with wastewater that had daily fluctuations in seawater content (7.5 to 22.5 g/L) and o...
Wastewater stressors shape the microbiome of aerobic granular sludge
Miranda, Catarina; Maia, Alexandra S.; Tiritan, Maria Elizabeth; Amorim, Catarina L.; Castro, Paula M. L.Aerobic granular sludge (AGS) is a revolutionary biological treatment technology, which, due to its competitive advantages, has been increasingly implemented in full-scale wastewater treatment plants (WWTPs). WWTPs are frequently challenged by multiple stressors, including pharmaceuticals and fluctuating salinity, which not only increase the complexity of wastewater but also impair the biological treatment perf...
Quantification of fluoroquinolones in wastewaters by liquid chromatography-tand...
Maia, Alexandra S.; Paíga, Paula; Delerue-Matos, Cristina; Castro, Paula M. L.; Tiritan, Maria ElizabethAntibiotics are the most consumed therapeutic classes worldwide and are released to the environment in their original form as well as potentially active metabolites and/or degradation products. Consequences of the occurrence of these compounds in the environment are primarily related to bacterial resistance development. This work presents a validated analytical method based on solid phase extraction (SPE) using...
Enantioselective degradation of ofloxacin and levofloxacin by the bacterial str...
Maia, Alexandra S.; Tiritan, Maria Elizabeth; Castro, Paula M.L.Fluoroquinolones are a class of antibiotics widely prescribed in both human and veterinary medicine of high environmental concern and characterized as environmental micropollutants due to their ecotoxicity and persistence and antibacterial resistance potential. Ofloxacin and levofloxacin are chiral fluoroquinolones commercialized as racemate and in enantiomerically pure form, respectively. Since the pharmacolog...
Occurrence of chiral bioactive compounds in the aquatic environment: a review
Ribeiro, Cláudia; Ribeiro, Ana Rita; Maia, Alexandra S.; Tiritan, Maria ElizabethIn recent decades, the presence of micropollutants in the environment has been extensively studied due to their high frequency of occurrence, persistence and possible adverse effects to exposed organisms. Concerning chiral micropollutants in the environment, enantiomers are frequently ignored and enantiomeric composition often neglected. However, enantioselective toxicity is well recognized, highlighting the ne...
Enantiomeric separation of tramadol and Its metabolites: method validation and ...
Silva, Cátia; Ribeiro, Cláudia; Maia, Alexandra S.; Gonçalves, Virgínia; Tiritan, Maria Elizabeth; Afonso, CarlosThe accurate assessment of racemic pharmaceuticals requires enantioselective analytical methods. This study presents the development and validation of an enantioselective liquid chromatography with a fluorescence detection method for the concomitant quantification of the enantiomers of tramadol and their metabolites, N-desmethyltramadol and O-desmethyltramadol, in wastewater samples. Optimized conditions were a...
Chiral analysis of pesticides and drugs of environmental concern: biodegradatio...
Maia, Alexandra S.; Ribeiro, Ana R.; Castro, Paula M. L.; Tiritan, Maria ElizabethThe importance of stereochemistry for medicinal chemistry and pharmacology is well recognized and the dissimilar behavior of enantiomers is fully documented. Regarding the environment, the significance is equivalent since enantiomers of chiral organic pollutants can also differ in biodegradation processes and fate, as well as in ecotoxicity. This review comprises designed biodegradation studies of several chira...
Integrated liquid chromatography method in enantioselective studies: biodegrada...
Maia, Alexandra S.; Castro, Paula M. L.; Tiritan, M. E.tOfloxacin is a chiral fluoroquinolone commercialized as racemate and as its enantiomerically pure formlevofloxacin. This work presents an integrated liquid chromatography (LC) method with fluorescencedetection (FD) and exact mass spectrometry (EMS) developed to assess the enantiomeric biodegradationof ofloxacin and levofloxacin in laboratory-scale microcosms. The optimized enantioseparation condi-tions were ac...
Anthropogenic pressure in a Portuguese river: endocrine-disrupting compounds, t...
Ribeiro, Cláudia M. R.; Maia, Alexandra S.; Ribeiro, Ana R.; Couto, Cristina; Almeida, Agostinho A.; Santos, Mariana; Tiritan, Maria E.Natural organic compounds such as phytoestrogens and phytosterols found in various plants, as well as mycotoxins produced by fungi, can be found in aquatic environments. The aim of this study was to investigate the occurrence of three different classes of natural estrogenic compounds, i.e., phytoestrogens, phytosterols and mycotoxins, in estuarine water samples from the Ave River estuary. For that, water sample...