310 documents found, page 1 of 31
Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content
Yadró, Carlos A.; Lopes, Ana Rita; Henriques, Dora; Musin, Eduard; Wegener, Jakob; Pinto, M. AliceGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing...
Honey bee viruses in the yellow-legged hornet Vespa velutina (Lepelieter 1836):...
Rodríguez-Flores, M. Shantal; Lopes, Ana; Diéguez-Antón, Ana; Seijo-Coello, M. Carmen; Pinto, M. AliceApiaries in Galicia, northwestern Spain, are currently facing the invasive alien species Vespa velutina, which is well established in the region. The pressure on honey bee colonies is high, resulting in both economic and ecological losses. Honey bee colonies also face the challenge of viruses, which are becoming increasingly diverse. In recent years, honey bee viruses have been spreading across taxonomic groups...
Genomic insights into middle eastern honey bee subspecies: population structure...
Henriques, Dora; Yadró Garcia, Carlos A.; Honrado, Mónica; Amaral, Joana S.; Muz, Mustafa Necati; Muz, Dilek; Haddad, Nizar; Al Shagour, BananThe genetic patterns of Middle Eastern A. mellifera subspecies have been understudied, hindering a comprehensive understanding of honey bee evolutionary history. Here, we studied the genetic integrity of five Middle Eastern subspecies across a broad geographical range: Turkey (A. m. anatoliaca, N=97; A. m. caucasia, N=75; A. m. syriaca, N=18), Jordan and Lebanon (A. m. syriaca, N=238 and N=29), Iran (A. m. meda...
Deciphering the variation in cuticular hydrocarbon profiles of six European hon...
Rodríguez-León, Daniel Sebastián; Uzunov, Aleksandar; Costa, Cecilia; Elen, Dylan; Charistos, Leonidas; Galea, Thomas; Gabel, Martin; Scheiner, RicardaThe Western honey bee (Apis mellifera) subspecies exhibit local adaptive traits that evolved in response to the different environments that characterize their native distribution ranges. An important trait is the cuticular hydrocarbon (CHC) profile, which helps to prevent desiccation and mediate communication. We compared the CHC profiles of six European subspecies (A. m. mellifera, A. m. carnica, A. m. ligusti...
Maternal ancestry of honey bees on São Miguel-Azores (Portugal)
Costa, Maíra; Lopes, Ana; Doblas-Bajo, Mónica; Quaresma, Andreia; Barreira, Diogo; Henriques, Dora; Pinto, M. AliceThe Apis mellifera iberiensis was introduced to the Azores in the 16th century by Portuguese settlers likely from the northern part of mainland Portugal. Many years later, in the 1980s, occurred a new introduction using honey bee subspecies from C-lineage maternal ancestry. This left a C-lineage signature in the Azores, with some islands, such as Pico or Graciosa, showing high frequency of C-lineage maternal an...
Diversity patterns of P450 and ABC transporter genes in 17 honey bee subspecies
Li, Fernanda; Rosa-Fontana, Annelise; Yadró Garcia, Carlos A.; Rufino, José; Verbinnen, Gilles; Graaf, Dirk C.; Smet, Lina de; Pinto, M. AliceHoney bees (Apis mellifera) inhabit a vast geographical range, spanning diverse natural and agricultural ecosystems. They are exposed to different levels and types of natural (such as plant allelochemicals) and synthetic (such as pesticides) xenobiotics within this range. Several genes have been implicated in the resistance of insects to pesticides, including the P450 monooxygenases superfamily and ATP-binding ...
Insights into population structure and conservation status of north African hon...
Yadró Garcia, Carlos A.; Henriques, Dora; Haider, Yamina; Eissa, Asmaa Anwar; Aglagane, Abdessamad; Rufino, José; Nanetti, AntonioThe population structure of North African A. mellifera subspecies has been overlooked. Here, WG generated from drones were analyzed to assess population structure and the conservation status of three A-lineage subspecies: A. m. lamarckii (Egypt, 68 samples), A. m. intermissa (Algeria, 69 samples), and A. m. sahariensis (Algeria, 68 samples; Morocco, 26 samples). Population structure was assessed by SNMF and PCA...
Comparative Analysis of DNA Extraction Methods for Individual Varroa destructor
Costa, Maíra; Lopes, Ana; Yadró Garcia, Carlos A.; Vitrio, Nathalia; Gonçalves, Telma; Pinto, M. Alice; Henriques, DoraThe ectoparasitic mite Varroa destructor is one of the major honey bee threats and it is associated to population worldwide decline. Genetic analyses using the mtDNA of V. destructor are fundamental for establishing the taxonomy and distribution of the mites. Consequently, low-quality DNA can lead to inaccurate or inconsistent data, making genetic interpretation more challenging. In this study, we compared the ...
Honeybee gut microbiota as an emerging endpoint for pesticide risk assessments
Rosa-Fontana, Annelise; Aguado-López, Daniel; Jabal-Uriel, Clara; Martín-Hernández, Raquel; Higes, Mariano; Pinto, M. Alice; Henriques, DoraA recent roadmap for the integration of environmental microbiotas in risk assessments under the European Food Safety Authority (EFSA) remit has been published. Healthy honeybee gut microbiota has emerged as a promising avenue to protect bees against stressors. Honeybees exhibit a consistent core microbiota, and dysbiosis, as part of a multiple stressor system, may be an indicator of adverse scenarios. We theref...
Exploring population structure and adaptation in honey bee subspecies from sout...
Yadró Garcia, Carlos A.; Henriques, Dora; Cilia, Giovanni; Rufino, José; Martín-Hernández, Raquel; Nanetti, Antonio; Pinto, M. AliceGlacial refugia harbor populations with complex diversity patterns. In honey bees, the Iberian and Italian Peninsulas served as two of the most important glacial refugia in Europe. Here, we analyzed whole genomes generated from drones to infer population structure, genetic diversity, and the molecular basis of the local adaptation for the two native subspecies of these Peninsulas: A. m. iberiensis (N=86; M-line...