34 documents found, page 1 of 4
In vitro bone tissue engineering strategies: the relevance of cells and culturi...
Bastos, Ana Raquel Fernandes; Maia, F. Raquel; Oliveira, Joaquim M.; Reis, R. L.; Correlo, V. M.The most recent advances in bone tissue engineering (BTE) approaches step forward in the field of three-dimensional (3D) tissue models, enabling the development of more realistic tools to study bone disorders, such as osteoporosis. BTE field aims to mimic native bone tissue more truthfully, providing an appropriate environment for tissue regeneration and repair through the combination of 3D porous scaffolds, sp...
Characterization of iron oxide nanotubes obtained by anodic oxidation for biome...
Rangel, Rita de Cássia Reis; Rangel, André Luiz Reis; Silva, Kerolene Barboza da; Escada, Ana Lúcia do Amaral; Chaves, Javier Andres MunozTo improve the biocompatibility and bioactivity of biodegradable iron-based materials, nanostructured surfaces formed by metal oxides offer a promising strategy for surface functionalization. To explore this potential, iron oxide nanotubes were synthesized on pure iron (Fe) using an anodic oxidation process (50 Vâ 30 min, using an ethylene glycol solution containing 0.3% NH4F and 3% H2O, at a speed of 100 rpm)....
Hydroxyapatite/alginate/gellan gum inks with osteoconduction and osteogenic pot...
Bastos, Ana Raquel Fernandes; da Silva, Lucília Pereira; Maia, F. Raquel; Franco, Albina Ribeiro; Noro, Jennifer Martins; Silva, CarlaThere is a growing demand for engineered bone tissues custom-designed to match the patient-specific defect size and in vitro models for studying bone diseases and/or drug screening. Herein, we propose a bioprinted bone tissue construct using SaOs-2 cells within alginate/gellan gum/hydroxyapatite inks. Different ink formulations were developed with varying hydroxyapatite content and then evaluated for viscoelast...
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced p...
Radhouani, Hajer; Gonçalves, Cristiana; Maia, F. Raquel; Oliveira, Maria Eduarda Moreira Pinheiro Santos; Reis, R. L.; Oliveira, Joaquim M.Hydrogels based on natural polysaccharides can have unique properties and be tailored for several applications, which may be mainly limited by the fragile structure and weak mechanical properties of this type of system. We successfully prepared cryogels made of newly synthesized kefiran exopolysaccharide-chondroitin sulfate (CS) conjugate via carbodiimide-mediated coupling to overcome these drawbacks. The freez...
Nano-inks for tissue-engineering
Maia, F. Raquel; Reis, R. L.; Oliveira, Joaquim M.Bioprinting strategies can combine materials and cells to create automated and high-fidelity relevant three-dimensional (3D) structures. Although bioprinting can mimic some tissues' features that cannot be achieved using classic scaffolding strategies, there are still challenges to overcome. Despite few successful examples of ink development for microtissues fabrication, the bioprinting of complex tissues and o...
Protein-based materials as cancer in vitro models
Ramos, P.; Maia, F. Raquel; Reis, R. L.; Oliveira, Joaquim M.The extracellular matrix (ECM) plays an active role in tumor progression. Increasing evidence points out a permissive tumor microenvironment that enhances malignancy and makes drug targeting a more difficult task. Protein-based materials as a substrate for cell culture hydrogels offer the advantage of having the presence of cell-responsive sequences as opposed to synthetic polymer-based hydrogels. This is impor...
3D-bioprinted in vitro disease models
Maia, F. Raquel; Reis, R. L.; Oliveira, Joaquim M.The development of new and more efficient approaches to tackle a myriad of human diseases has been extensively pursued. The development of in vitro models has emerged as a promising approach capable to emulate important features of disease etiology and progression, facilitating the production and screening of new therapies. Recently, 3D bioprinting, an extension of additive manufacturing technique, has introduc...
Cell-derived matrices for scaffolds improvement
Maia, F. Raquel; Reis, R. L.; Oliveira, Joaquim M.Cells’ surrounding microenvironment is highly complex, comprising an organized macromolecular network. Additionally, it plays crucial structural and signaling roles for cellular functions and, ultimately, for tissues’ features. Current biomaterials used in tissue engineering applications fail to recapitulate this complex environment, prompting the pursuit of other alternatives. In this scenario, cell-derived ma...
Recent approaches towards bone tissue engineering
Maia, F. Raquel; Bastos, Ana Raquel Fernandes; Oliveira, J. M.; Correlo, V. M.; Reis, R. L.Bone tissue engineering approaches have evolved towards addressing the challenges of tissue mimetic requirements over the years. Different strategies have been combining scaffolds, cells, and biologically active cues using a wide range of fabrication techniques, envisioning the mimicry of bone tissue. On the one hand, biomimetic scaffold-based strategies have been pursuing different biomaterials to produce scaf...
Osteogenic lithium-doped brushite cements for bone regeneration
Hurle, K.; Maia, F. Raquel; Ribeiro, Viviana Pinto; Pina, Sandra Cristina Almeida; Oliveira, J. M.; Goetz-Neunhoeffer, F.; Reis, R. L.This study investigated the osteogenic performance of new brushite cements obtained from Li+-doped beta-tricalcium phosphate as a promising strategy for bone regeneration. Lithium (Li+) is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells (hASCs) and the osteogenic differentiation-related gene expression, essential for osteogenesis. In-situ X-ray diffraction (X...