5 documents found, page 1 of 1
Third-generation electrochemical biosensor based on nitric oxide reductase immo...
Gomes, Filipa O.; Maia, Luísa B.; Delerue-Matos, Cristina; Moura, Isabel; Moura, José J.G.; Morais, SimoneNitric oxide (NO) has a crucial role in signaling and cellular physiology in humans. Herein, a novel third-generation biosensor based on the Marinobacter hydrocarbonoclasticus metalloenzyme (nitric oxide reductase (NOR)), responsible for the NO reduction in the denitrifying processes, was developed through the direct adsorption of a new nanocomposite (multiwalled carbon nanotubes (MWCNTs)/1-n-butyl-3-methylimid...
Third-generation electrochemical biosensor based on nitric oxide reductase immo...
Gomes, Filipa O.; Maia, Luísa B.; Delerue-Matos, Cristina; Moura, Isabel; Moura, José J.G.; Morais, SimoneNitric oxide (NO) has a crucial role in signaling and cellular physiology in humans. Herein, a novel third-generation biosensor based on the Marinobacter hydrocarbonoclasticus metalloenzyme (nitric oxide reductase (NOR)), responsible for the NO reduction in the denitrifying processes, was developed through the direct adsorption of a new nanocomposite (multiwalled carbon nanotubes (MWCNTs)/1-n-butyl-3-methylimid...
Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric...
Gomes, Filipa O.; Maia, Luísa B.; Loureiro, Joana A.; Pereira, Maria Carmo; Delerue-Matos, Cristina; Moura, Isabel; Moura, José J.G.; Morais, SimoneAn enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), ...
Electroanalytical characterization of the direct Marinobacter hydrocarbonoclast...
Gomes, Filipa O.; Maia, Luísa B.; Cordas, Cristina; Moura, Isabel; Delerue-Matos, Cristina; Moura, José J.G.; Morais, SimoneUnderstanding the direct electron transfer processes between redox proteins and electrode surface is fundamental to understand the proteins mechanistic properties and for development of novel biosensors. In this study, nitric oxide reductase (NOR) extracted from Marinobacter hydrocarbonoclasticus bacteria was adsorbed onto a pyrolytic graphite electrode (PGE) to develop an unmediated enzymatic biosensor (PGE/NO...
Nitric Oxide Detection Using Electrochemical Third-generation Biosensors - Base...
Gomes, Filipa O.; Maia, Luísa B.; Cordas, Cristina; Delerue-Matos, Cristina; Moura, Isabel; Moura, José J. G.; Morais, SimoneNitric oxide radical (NO) is a signalling molecule involved in virtually all forms of life. Its relevance has been leading to the development of different analytical methodologies to assess the temporal and spatial fluxes of NO under the complex biological milieu. Third‐generation electrochemical biosensors are promising tools for in loco and in vivo NO quantification and, over the past years, heme proteins and...