6 documents found, page 1 of 1
Genetic Transformation of Quercus ilex Somatic Embryos with a Gnk2-like Protein...
Serrazina, S.; Martinez, M.T.; Cano, V.; Malhó, R.; Costa, R.L.; Corredoira, E.Holm oak is a key tree species in Mediterranean ecosystems, whose populations have been increasingly threatened by oak decline syndrome, a disease caused by the combined action of Phytophthora cinnamomi and abiotic stresses. The aim of the present study was to produce holm oak plants that overexpress the Ginkbilobin-2 homologous domain gene (Cast_Gnk2-like) that it is known to possess antifungal properties. Pro...
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ec...
Sebastiana, M.; Gargallo-Garriga, A.; Sardans, J.; Pérez-Trujillo, M.; Monteiro, Filipa; Figueiredo, A.; Maia, M.; Nascimento, R.; Silva, M. SousaMycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized by the ectomycorrhizal fungus Pisolithus tinctorius were compared with non-colonized roots. Results show that compounds pu...
In vitro functional characterization of missense mutations in the LDLR gene
Silva, S.; Alves, A.C.; Patel, D.; Malhó, R.; Soutar, A.K.; Bourbon, M.Mutations in the LDL receptor gene are the major cause of familial hypercholesterolaemia (FH) but it has been previously shown that the simple finding of a variation in the coding sequence of the LDLR does not confirm that it is the actual cause of FH. The pathogenicity of five missense alterations in the LDLR gene coding sequence found in a previous epidemiologic study was investigated.
In silico versus in vitro analysis of LDLR mutations
Alves, A.C.; Silva, S.; Patel, D.; Malhó, R.; Soutar, A.K.; Bourbon, M.The LDL receptor (LDLR) is a glycoprotein that mediates binding and internalization of cholesterol-rich lipoproteins from plasma. Mutations in the LDLR gene are the major cause of familial hypercholesterolaemia (FH), which results in impaired catabolism of circulating LDL. This common autosomal inherited metabolism disorder leads to premature atherosclerosis and increased risk of CHD. Many different mutations (...
Ceruloplasmin expression by human peripheral blood lymphocytes: a new link betw...
Banha, J.; Marques, L.; Oliveira, R.; Martins, M. de F.; Paixão, E.; Pereira, D.; Malhó, R.; Penque, D.; Costa, L.Ceruloplasmin (CP) is a multicopper oxidase involved in the acute phase reaction to stress. Although the physiological role of CP is uncertain, its role in iron (Fe) homeostasis and protection against free radical-initiated cell injury has been widely documented. Previous studies showed the existence of two molecular isoforms of CP: secreted CP (sCP) and a membrane glycosylphosphatidylinositol (GPI)-anchored fo...
Signalling pathways in pollen germination and tube growth
Malhó, R.; Liu, Q.; Monteiro, D.; Rato, C.; Camacho, L.; Dinis, A.Summary. Signalling is an integral component in the establishment and maintenance of cellular identity. In plants, tip-growing cells represent an ideal system to investigate signal transduction mechanisms, and among these, pollen tubes (PTs) are one of the favourite models. Many signalling pathways have been identified during germination and tip growth, namely, Ca2+, calmodulin, phosphoinositides, protein kinas...