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Isolation Method and Characterization of Outer Membranes Vesicles of Helicobact...

Melo, J; Pinto, V; Fernandes, T; Malheiro, AR; Osorio, H; Figueiredo, C; Leite, M

Outer membrane vesicles (OMVs) are small vesicles constitutively shed by all Gram-negative bacterium, which have been proposed to play a role in Helicobacter pylori persistence and pathogenesis. The methods currently available for the isolation of H. pylori OMVs are diverse and time-consuming, raising the need for a protocol standardization, which was the main aim of this study. Here, we showed that the chemica...


Cross-contamination of lettuce with Campylobacter spp. via cooking salt during ...

Santos-Ferreira, N; Alves, A; Cardoso, MJ; Langsrud, S; Malheiro, AR; Fernandes, R; Maia, R; Truninger, M; Junqueira, L; Nicolau, AI; Dumitrascu, L

Campylobacter spp. are the most common bacterial pathogens associated with human gastroenteritis in industrialized countries. Contaminated chicken is the food vehicle associated with the majority of reported cases of campylobacteriosis, either by the consumption of undercooked meat or via cross- contamination of ready-to-eat (RTE) foods during the handling of contaminated raw chicken parts and carcasses. Our re...


Thermo-mechanical behaviour of human nasal cartilage

Fertuzinhos, A; Teixeira, MA; Ferreira, MG; Fernandes, R; Correia, R; Malheiro, AR; Flores, P; Zille, A; Dourado, N

The aim of this study was to undergo a comprehensive analysis of the thermo-mechanical properties of nasal cartilages for the future design of a composite polymeric material to be used in human nose reconstruction surgery. A thermal and dynamic mechanical analysis (DMA) in tension and compression modes within the ranges 1 to 20 Hz and 30 °C to 250 °C was performed on human nasal cartilage. Differential scanning...


Peripheral nervous system plasmalogens regulate Schwann cell differentiation an...

Silva, TF; Eira, J; Lopes, AT; Malheiro, AR; Sousa, V; Luoma, A; Avila, RL; Wanders, RJ; Just, WW; Kirschner, DA; Sousa, MM; Brites, P

Rhizomelic chondrodysplasia punctata (RCDP) is a developmental disorder characterized by hypotonia, cataracts, abnormal ossification, impaired motor development, and intellectual disability. The underlying etiology of RCDP is a deficiency in the biosynthesis of ether phospholipids, of which plasmalogens are the most abundant form in nervous tissue and myelin; however, the role of plasmalogens in the peripheral ...


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