14 documents found, page 1 of 2
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp s...
Machado, A.; Pereira, Isabel; Pereira, José Eduardo; Maltez, Luís; Brandão, Ana; Alvites, Rui; Sousa, Ana Catarina; Branquinho, MarianaRegeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release,...
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp s...
Machado, Alexandra; Pereira, Isabel; Pereira, José Eduardo; Maltez, Luís; Brandão, Ana; Alvites, Rui; Sousa, Ana Catarina; Branquinho, MarianaRegeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250–500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release...
High frequency of the EMRSA-15 clone (ST22-MRSA-IV) in hospital wastewater
Silva, Vanessa; Ribeiro, Jessica; Rocha, Jaqueline; Manaia, Célia M.; Silva, Adriana; Pereira, José Eduardo; Maltez, Luís; Capelo, José LuisHospital wastewaters often carry multidrug-resistant bacteria and priority pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA). Pathogens and antibiotic resistance genes present in wastewaters may reach the natural environment facilitating their spread. Thus, we aimed to isolate MRSA from wastewater of 3 hospitals located in the north of Portugal and to characterize the isolates regarding the ...
Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus fr...
Silva, Vanessa; Caniça, Manuela; Manageiro, Vera; Vieira-Pinto, Madalena; Pereira, José Eduardo; Maltez, Luís; Poeta, Patrícia; Igrejas, GilbertoSeveral studies have showed that a dog-to-human transmission of Staphylococcus aureus occurs. Hunting dogs do not have as much contact with their owners as dogs that live in the same household as the owners; however, these dogs have contact with their owners during hunting activities as well as when hunting game; therefore, we aimed to isolate S. aureus from hunters and their hunting dogs to investigate a possi...
Nocturnal Birds of Prey as Carriers of Staphylococcus aureus and Other Staphylo...
Silva, Vanessa; Lopes, Ana Filipa; Soeiro, Vanessa; Caniça, Manuela; Manageiro, Vera; Pereira, José Eduardo; Maltez, Luís; Capelo, José LuisOwls are nocturnal predators that inhabit urbanized and farmlands. They are in direct contact with other animals, both livestock and small wild rodents that they mostly feed on. Staphylococci can be both commensal and pathogenic bacteria that are widespread across the various ecological niches. We aimed to isolate staphylococci from owls and to characterize their antimicrobial resistance, virulence factors and ...
Regeneration of critical-sized defects, in a goat model, using a dextrin-based ...
Pereira, Isabel Sofia Melo; Pereira, José Eduardo; Maltez, Luís; Rodrigues, Alexandra; Rodrigues, Catarina; Oliveira, Manuela; Silva, Dina M.The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have ar...
High efficacy of ozonated oils on the removal of biofilms produced by methicill...
Silva, Vanessa; Peirone, Cecília; Amaral, Joana S.; Capita, Rosa; Alonso-Calleja, Carlos; Marques-Magallanes, José A.; Martins, Ângela; Carvalho, Águeda© 2020 by the authors. Ozone has a high wound healing capacity and antibacterial properties and can be used as a complementary treatment in infections. Methicillin-resistant S. aureus (MRSA) is the most common pathogen found in infected diabetic foot ulcers. Most of MRSA are resistant to several classes of antibiotics and, therefore, there is a need for new, effective, and well-tolerated agents. Thus, we aimed ...
Genetic characterization of methicillin-resistant staphylococcus aureus isolate...
Silva, Vanessa; Hermenegildo, Sara; Ferreira, Catarina; Manaia, Celia M.; Capita, Rosa; Alonso-Calleja, Carlos; Carvalho, Isabel; Pereira, José EduardoIn this study we aimed to characterize antimicrobial resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bloodstream infections as well as the associated genetic lineages of the isolates. Sixteen MRSA isolates were recovered from bacteremia samples from inpatients between 2016 and 2019. The antimicrobial susceptibility of these isolates was tested by the Kirby–Bauer disk diffusion met...
In vivo systemic toxicity assessment of an oxidized dextrin‐based hydrogel and ...
Pereira, Isabel; Fraga, Sónia; Maltez, Luís; Requicha, João; Guardão, Luísa; Oliveira, Joana; Prada, Justina; Alves, Helena; Santos, José DomingosThe worldwide incidence of bone disorders is raising, mainly due to aging population. The lack of effective treatments is pushing the development of synthetic bone substitutes (SBSs). Most ceramic-based SBSs commercially available display limited handling properties. Attempting to solve these issues and achieve wider acceptance by the clinicians, granular ceramics have been associated with hydrogels (HGs) to pr...
Diversity of methicillin-resistant staphylococci among wild Lepus granatensis: ...
Silva, Vanessa; Pereira, José Eduardo; Maltez, Luís; Ferreira, Eugénia; Manageiro, Vera; Caniça, Manuela; Capelo, José L.; Igrejas, GilbertoMRSA in humans, pets and livestock have been widely investigated, nevertheless, there is still little information of MRSA in wild animals. Therefore, this study aimed to investigate the occurrence and antimicrobial resistance profiles of methicillin-resistant staphylococci (MRS) in wild Iberian hares and to characterize their genetic lineages. Samples from 83 wild hares (Lepus granatensis) were collected during...