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One-step immobilization and stabilization of a recombinant Enterococcus faecium...
Sousa, Marylane de; Melo, Vânia M. M.; Hissa, Denise Cavalcante; Manzo, Ricardo M.; Mammarella, Enrique J.; Antunes, André Saraiva Leão MarceloA recombinant L-arabinose isomerase from Enterococcus faecium DBFIQ E36 was immobilized onto multifunctional epoxide supports by chemical adsorption and onto a chelate-activated support via polyhistidine-tag, located on the N-terminal (N-His-L-AI) or on the C-terminal (C-His-L-AI) sequence, followed by covalent bonding between the enzyme and the support. The results were compared to reversible L-AI immobilizati...
Date: 2021
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Origin: Oasisbr