80 documents found, page 1 of 8
Glial-restricted precursors stimulate endogenous cytogenesis and effectively re...
Martins-Macedo, Joana; Araújo, Bruna; Anjo, Sandra I.; Silveira-Rosa, Tiago; Patrício, Patrícia; Alves, Nuno Dinis; Silva, Joana M.; Teixeira, Fábio G.Adult cytogenesis, the continuous generation of newly-born neurons (neurogenesis) and glial cells (gliogenesis) throughout life, is highly impaired in several neuropsychiatric disorders, such as Major Depressive Disorder (MDD), impacting negatively on cognitive and emotional domains. Despite playing a critical role in brain homeostasis, the importance of gliogenesis has been overlooked, both in healthy and dise...
Proteomic and Metabolic Analysis of Pinus halepensis Mill. Embryonal Masses Ind...
Pereira, Cátia; Castander-Olarieta, Ander; Montalbán, Itziar A.; Mendes, Vera M.; Correia, Sandra; Pedrosa, Ana; Manadas, Bruno; Moncaleán, PalomaUnderstanding the physiological and molecular adjustments occurring during tree stress response is of great importance for forest management and breeding programs. Somatic embryogenesis has been used as a model system to analyze various processes occurring during embryo development, including stress response mechanisms. In addition, "priming" plants with heat stress during somatic embryogenesis seems to favor t...
Enrichment of hepatic glycogen and plasma glucose from H₂18 O informs gluconeog...
Coelho, Margarida; Mahar, Rohit; Belew, Getachew D.; Torres, Alejandra; Barosa, Cristina; Cabral, Fernando; Viegas, Ivan; Gastaldelli, AmaliaDeuterated water (2 H2 O) is a widely used tracer of carbohydrate biosynthesis in both preclinical and clinical settings, but the significant kinetic isotope effects (KIE) of 2 H can distort metabolic information and mediate toxicity. 18 O-water (H2 18 O) has no significant KIE and is incorporated into specific carbohydrate oxygens via well-defined mechanisms, but to date it has not been evaluated in any animal...
Biomarkers of hypoxic-ischemic encephalopathy: a systematic review
Caramelo, Inês; Coelho, Margarida; Rosado, Miguel; Cardoso, Carla M P; Dinis, Alexandra; Duarte, Carlos B.; Grãos, Mário; Manadas, BrunoBackground Current diagnostic criteria for hypoxic–ischemic encephalopathy in the early hours lack objective measurement tools. Therefore, this systematic review aims to identify putative molecules that can be used in diagnosis in daily clinical practice (PROSPERO ID: CRD42021272610). Data sources Searches were performed in PubMed, Web of Science, and Science Direct databases until November 2020. English origin...
Chronic treatment with D2-antagonist haloperidol leads to inhibitory/excitatory...
Santa, Cátia; Rodrigues, Diana; Coelho, Joana F.; Anjo, Sandra I.; Mendes, Vera M.; Bessa-Neto, Diogo; Dunn, Michael J.; Cotter, David; Baltazar, GraçaStriatal dysfunction has been implicated in the pathophysiology of schizophrenia, a disorder characterized by positive symptoms such as hallucinations and delusions. Haloperidol is a typical antipsychotic medication used in the treatment of schizophrenia that is known to antagonize dopamine D2 receptors, which are abundantly expressed in the striatum. However, haloperidol's delayed therapeutic effect also sugge...
Self-recycling and partially conservative replication of mycobacterial methylma...
Maranha, Ana; Costa, Mafalda; Ripoll-Rozada, Jorge; Manso, José A.; Miranda, Vanessa; Mendes, Vera M.; Manadas, Bruno; Macedo-Ribeiro, SandraThe steep increase in nontuberculous mycobacteria (NTM) infections makes understanding their unique physiology an urgent health priority. NTM synthesize two polysaccharides proposed to modulate fatty acid metabolism: the ubiquitous 6-O-methylglucose lipopolysaccharide, and the 3-O-methylmannose polysaccharide (MMP) so far detected in rapidly growing mycobacteria. The recent identification of a unique MMP methyl...
New Insights on Sperm Function in Male Infertility of Unknown Origin: A Multimo...
Pacheco, Rita I.; Cristo, Maria I.; Anjo, Sandra I.; Silva, Andreia F.; Sousa, Maria Inês; Santos Tavares, Renata; Sousa, Ana PaulaThe global trend of rising (male) infertility is concerning, and the unidentifiable causes in half of the cases, the so-called unknown origin male infertility (UOMI), demands a better understanding and assessment of both external/internal factors and mechanisms potentially involved. In this work, it was our aim to obtain new insight on UOMI, specifically on idiopathic (ID) and Unexplained male infertility (UMI)...
Proteomics Reveals mRNA Regulation and the Action of Annexins in Thyroid Cancer
Coelho, Margarida; Capela, João; Anjo, Sandra I.; Pacheco, João; Fernandes, Margarida Sá; Amendoeira, Isabel; Jones, John G.; Raposo, LuísDifferentiated thyroid cancer is the most common malignancy of the endocrine system. Although most thyroid nodules are benign, given the high incidence of thyroid nodules in the population, it is important to understand the differences between benign and malignant thyroid cancer and the molecular alterations associated with malignancy to improve detection and signal potential diagnostic, prognostic, and therape...
Saliva for diabetes’ complications: non-invasive diagnostics and monitoring
Esteves, Eduardo; Mendes, Vera; Manadas, Bruno; Lemos, António; Alves, Dora; Bernardino, Liliana; Correia, Maria; Barros, Marlene; Esteves, Ana C.Type 2 Diabetes Mellitus is a chronic metabolic disease that poses a significant long-term health risk due to its debilitating complications. While the diagnosis and monitoring of diabetes are well-established, the monitoring of its numerous complications presents a challenge, which can compromise patients' prognosis and quality of life. We analyzed the proteome of ten saliva samples, five from healthy individu...
A peptide-centric approach to analyse quantitative proteomics data- an applicat...
Lima, Tania; Rodrigues, João Eduardo; Manadas, Bruno; Henrique, Rui; Fardilha, Margarida; Vitorino, RuiBottom-up proteomics is a popular approach in molecular biomarker research. However, protein analysts have realized the limitations of protein-based approaches for identifying and quantifying proteins in complex samples, such as the identification of peptides sequences shared by multiple proteins and the difficulty in identifying modified peptides. Thus, there are many exciting opportunities to improve analysis...