20 documents found, page 1 of 2
Oral cortisol and dexamethasone intake: Differential physiology and transcripti...
Barany, A.; Fuentes, Juan; Valderrama, V.; Broz-Ruiz, A.; Martínez-Rodríguez, G.; Mancera, J. M.This study approached the long-term oral administration of cortisol (F) and dexamethasone (DEX), two synthetic glucocorticoids, compared to a control group (CT) in the juveniles of a marine teleost, the gilthead seabream (Sparus aurata). Physiologically, DEX treatment impaired growth, which appears to be linked to carbohydrate allocation in muscle and liver, hepatic triglycerides depletion, and reduced hematocr...
Ocean acidification compromises energy management in Sparus aurata (Pisces: Tel...
Ruiz-Jarabo, I.; Gregorio, SF; Alves, Alexandra; Mancera, J. M.; Fuentes, JuanThe effects of ocean acidification mediated by an increase in water pCO(2) levels on marine organisms are currently under debate. Elevated CO2 concentrations in the seawater induce several physiological responses in teleost fish, including acid-base imbalances and osmoregulatory changes. However, the consequences of CO2 levels enhancement on energy metabolism are mostly unknown. Here we show that 5 weeks of exp...
Tissue and salinity specific Na+/Cl− cotransporter (NCC) orthologues involved i...
Barany, A.; Shaughnessy, C. A.; Pelis, R. M.; Fuentes, J.; Mancera, J. M.; McCormick, S. D.Two orthologues of the gene encoding the Na+-Cl− cotransporter (NCC), termed ncca and nccb, were found in the sea lamprey genome. No gene encoding the Na+-K+-2Cl cotransporter 2 (nkcc2) was identifed. In a phylogenetic comparison among other vertebrate NCC and NKCC sequences, the sea lamprey NCCs occupied basal positions within the NCC clades. In freshwater, ncca mRNA was found only in the gill and nccb only in...
Effect of amino acid supplementation and stress on expression of molecular mark...
Herrera, M.; Matias, A. C.; Soares, F.; Ribeiro, L.; Moreira, Márcio; Salamanca, N.; Jerez-Cepa, I.; Mancera, J. M.; Astola, A.The objectives of this work were: 1) develop of molecular stress biomarkers obtaining sequence data of different transcripts, 2) study the molecular stress response through the expression quantification of key gene involved in it, and 3) assess the effects of dietary amino acid additives on stress response in meagre meagres (Argyrososmus regius). Fish batches were fed two experimental diets with tryptophan (Trp...
Dietary aflatoxin B1 (AFB1) reduces growth performance, impacting growth axis, ...
Barany, A.; Guilloto, M.; Cosano, J.; de Boevre, M.; Oliva, M.; de Saeger, S.; Fuentes, J.; Martinez-Rodriguez, G.; Mancera, J. M.Mycotoxins are an increasing threat to all the related commodities from agriculture. Its occurrence is expected to increase due to climate change. Here, we examined the impacts of dietary toxicity of aflatoxin B1 (AFB1) in gilthead sea bream (Sparus aurata) at levels of 1 or 2 mg AFB1 kg(-1)- fish feed. Inclusion of AFB1 in the diet resulted in 80% inhibition of the total weight gain during the 85-day trial. Ca...
Survival rates and physiological recovery responses in the lesser-spotted catsh...
Barragan-Mendez, C.; Ruiz-Jarabo, I.; Fuentes, Juan; Mancera, J. M.; Sobrino, I.In 2019, Europe will adopt a no-discards policy in fisheries. This entails the landing of captured species unless strong evidence is provided supporting their survival and recovery after fishing. Thus, research on this topic is gaining momentum. Bottom-trawling, as a non-selective fishing method, is characterized by a high proportion of discards including vulnerable key species, such as demersal sharks. Their s...
Intestinal response to salinity challenge in the Senegalese sole (Solea senegal...
Ruiz-Jarabo, I.; Barany, A.; Jerez-Cepa, I.; Mancera, J. M.; Fuentes, JuanFish are continuously forced to actively absorb or expel water and ions through epithelia. Most studies have focused on the gill due to its role in Na+ and Cl- trafficking. However, comparatively few studies have focused on the changing function of the intestine in response to external salinity. Therefore, the present study investigated the main intestinal changes of long-term acclimation of the Senegalese sole...
Characterization of the peripheral thyroid system of gilthead seabream acclimat...
Ruiz-Jarabo, I.; Klaren, P. H. M.; Louro, Bruno; Martos-Sitcha, J. A.; Pinto, P. I.; Vargas-Chacoff, L.; Flik, G.; Martinez-Rodriguez, G.Thyroid hormones are involved in many developmental and physiological processes, including osmoregulation. The regulation of the thyroid system by environmental salinity in the euryhaline gilthead seabream (Sparus aurata) is still poorly characterized. To this end seabreams were exposed to four different environmental salinities (5, 15, 40 and 55 ppt) for 14 days, and plasma free thyroid hormones (fT3, ff4), ou...
Metabolic responses to salinity changes in the subantarctic notothenioid teleos...
Vargas-Chacoff, L.; Moneva, F.; Oyarzun, R.; Martinez, D.; Saavedra, E.; Ruiz-Jarabo, I.; Munoz, J. L. P.; Bertran, C.; Mancera, J. M.Eleginops maclovinus is an endemic, subantarctic Notothenioidei species. This study examined the influence of different environmental salinities (5, 15, and 45 psu; and 32 psu as a control) on energy metabolism in E. maclovinus over a period of 14 days. Metabolite contents and enzymatic activities related to carbohydrate, amino acid, and lipid metabolisms were evaluated in metabolic (liver) and osmoregulatory (...
AVT is involved in the regulation of ion transport in the intestine of the sea ...
Martos-Sitcha, J. A.; Gregorio, Silvia; Carvalho, Edison Samir Mascarelhas; Canario, Adelino V. M.; Power, Deborah; Mancera, J. M.The intestine of marine fish plays a crucial role in ion homeostasis by selective processing of ingested fluid. Although arginine vasotocin (AVT) is suggested to play a role in ion regulation in fish, its action in the intestine has not been demonstrated. Thus, the present study investigated in vitro the putative role of AVT in intestinal ion transport in the sea bream (Sparus aurata). A cDNA encoding part of a...