6 documents found, page 1 of 1
Next-generation proteomics reveals a greater antioxidative response to drought ...
Marques, Isabel; Gouveia, Duarte; Gaillard, Jean-Charles; Martins, Sónia; Semedo, Magda C.; Lidon, Fernando C.; DaMatta, Fabio; Ribeiro, AnaDrought is a major threat to coffee, compromising the quality and quantity of its production. We have analyzed the core proteome of 18 Coffea canephora cv. Conilon Clone 153 and C. arabica cv. Icatu plants and assessed their responses to moderate (MWD) and severe (SWD) water deficits. Label-free quantitative shotgun proteomics identified 3000 proteins in both genotypes, but less than 0.8% contributed to ca. 20%...
Protective Responses at the Biochemical and Molecular Level Differ between a Co...
Vinci, Gabriella; Marques, Isabel; Rodrigues, Ana P.; Martins, Sónia; Leitão, António E.; Semedo, Magda C.; Silva, Maria J.; Lidon, Fernando C.Climate changes with global warming associated with rising atmospheric [CO2] can strongly impact crop performance, including coffee, which is one of the most world’s traded agricultural commodities. Therefore, it is of utmost importance to understand the mechanisms of heat tolerance and the potential role of elevated air CO2 (eCO2) in the coffee plant response, particularly regarding the antioxidant and other p...
Next-Generation Proteomics Reveals a Greater Antioxidative Response to Drought ...
Marques, Isabel; Gouveia, Duaret; Gaillard, Jean-Charles; Martins, Sónia; Semedo, Magda C.; Lidon, Fernando C.; DaMatta, Fábio M.Drought is a major threat to coffee, compromising the quality and quantity of its production. We have analyzed the core proteome of 18 Coffea canephora cv. Conilon Clone 153 and C. arabica cv. Icatu plants and assessed their responses to moderate (MWD) and severe (SWD) water deficits. Label-free quantitative shotgun proteomics identified 3000 proteins in both genotypes, but less than 0.8% contributed to ca. 20%...
Intrinsic non-stomatal resilience to drought of the photosynthetic apparatus in...
JN; Rodrigues, Ana P.; Lidon, Fernando C.; P. Pais, Isabel; Marques, Isabel; Gouveia, Duarte; ARMENGAUD, Jean; Silva, Maria J.; Martins, SóniaGrowing water restrictions associated with climate changes constitute daunting challenges to crop performance. This study unveils the impacts of moderate (MWD) or severe (SWD) water deficit, and their interaction with air [CO2], on the photosynthetic apparatus of Coffea canephora Pierre ex A. Froehner cv. Conilon Clone 153 (CL153) and Coffea arabica L. cv. Icatu. Seven year-old potted plants grown under 380 (aC...
Resilient and sensitive key points of the photosynthetic machinery of coffea sp...
Dubberstein, Danielly; Lidon, Fernando C.; Rodrigues, Ana P.; Semedo, José N.; Marques, Isabel; Rodrigues, Weverton P.; Gouveia, Duarte; ARMENGAUD, JeanThis study unveils the single and combined drought and heat impacts on the photosynthetic performance ofCoffea arabicacv. Icatu andC. canephoracv. Conilon Clone 153 (CL153). Well-watered (WW) potted plants were gradually submitted to severe water deficit (SWD) along 20 days under adequate temperature (25/20 degrees C, day/night), and thereafter exposed to a gradual temperature rise up to 42/30 degrees C, follow...
Adsorption of myoglobin on calixarenes and biocatalysis in organic media
Semedo, Magda C.; Karmali, Amin; Barata, Patrícia; Prata, José V.Derivatives of p-tert-butylcalix[4,6,8]arene carboxylic acids were used for selective adsorption of myoglobin.Amixtureofmyoglobin,laccaseandperoxidase wasusedforextractionwithcalixarenesandonlymyoglobin was selectively extracted to organic media. Myoglobin and Mb c–calixarene exhibited pseudoactivity of peroxidase in aqueous and organic media. This protein-calixarene complex exhibited the highest specific activi...