2 documents found, page 1 of 1
Variability in the recognition of distinctive immunofluorescence patterns in di...
Dellavance,Alessandra; Cruvinel,Wilson de Melo; Francescantonio,Paulo Luiz Carvalho; Mangueira,Cristovão Luis Pitangueira; Drugowick,Inês CristinaINTRODUCTION: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for the detection of autoantibodies against cellular antigens. However, the culture conditions, cell fixation and permeabilization processes interfere directly in the preservation and spatial distribution of antigens. Therefore, one can assume that certain peculiarities in the processing of cellular substrate may affect the...
Correlation between flow cytometry and histologic findings: ten year experience...
Bezerra,Alanna Mara Pinheiro Sobreira; Pasqualin,Denise da Cunha; Guerra,João Carlos de Campos; Colombini,Marjorie ParisObjective: To demonstrate the advantages of correlating flow cytometry immunophenotyping with the pathology/ immunohistochemistry of lymph nodes or nodules in the diagnosis of lymphoproliferative diseases. Methods: A retrospective study was carried out of 157 biopsy or fine-needle aspiration lymph nodes/ nodule specimens taken from 142 patients, from 1999 and 2009. The specimens were simultaneously studied with...