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Thyroid disruption in the lizard Podarcis bocagei exposed to a mixture of herbi...

Bicho, Rita C.; Amaral, Maria José; Faustino, Augusto M. R.; Power, Deborah M.; Rêma, Alexandra; Carretero, Miguel A.; Soares, Amadeu M. V. M.

Pesticide exposure has been related with thyroid disrupting effects in different vertebrate species. However, very little is known about the effects of these compounds in reptiles. In the Mediterranean area, lacertid lizards are the most abundant vertebrate group in agroecosystems, and have been identified as potential model species for reptile ecotoxicology. The aim of this study was to understand if the herbi...


Thyroid disruption in the lizard Podarcis bocagei exposed to a mixture of herbi...

Bicho, Rita C.; Amaral, Maria José; Faustino, Augusto M. R.; Power, Deborah; Rêma, Alexandra; Carretero, Miguel A.; Soares, Amadeu M. V. M.

Pesticide exposure has been related with thyroid disrupting effects in different vertebrate species. However, very little is known about the effects of these compounds in reptiles. In the Mediterranean area, lacertid lizards are the most abundant vertebrate group in agroecosystems, and have been identified as potential model species for reptile ecotoxicology. The aim of this study was to understand if the herbi...


Biomarkers of exposure and effect in a lacertid lizard (Podarcis bocagei Seoane...

Amaral, Maria José; Sanchez-Hernandez, Juan C.; Bicho, Rita C.; Carretero, Miguel A.; Valente, Ricardo; Faustino, Augusto M. R.; Soares, Amadeu M. V. M.

In Europe, reptiles have been recently included in environmental risk-assessment processes for registration of plant-protection products. However, data on toxicity effects of most compounds are lacking. Chlorpyrifos is the most commonly used organophosphorus insecticide worldwide. In the present study, the authors exposed a lacertid lizard, Podarcis bocagei, to sublethal concentrations of chlorpyrifos. Individu...


The usefulness of mesocosms for ecotoxicity testing with lacertid lizards

Amaral, Maria José; Bicho, Rita C.; Carretero, Miguel A.; Sanchez-Hernandez, Juan C.; Faustino, Augusto; Soares, Amadeu M. V. M.; Mann, Reinier M.

Mesocosms (i.e., outdoor, man-made representations of natural ecosystems) have seldom been used to study the impact of contaminants on terrestrial ecosystems. However, mesocosms can be a useful tool to provide a link between field and laboratory studies. We exposed juvenile lacertid lizards for a period of over one year to pesticides (herbicides and insecticides) in mesocosm enclosures with the intention of val...


The use of a lacertid lizard as a model for reptile ecotoxicology studies - par...

Amaral, Maria José; Carretero, Miguel A.; Bicho, Rita C.; Soares, Amadeu M. V. M.; Mann, Reinier M.

At the European level, lacertid lizards have been proposed as potential model species for reptile ecotoxicology. We studied demographic and morphological aspects of natural field subpopulations of Podarcis bocagei inhabiting similar agricultural habitats which were either regularly exposed to pesticides, or not. Parameters examined in this study included population size and density, sex ratio, adult body size, ...


The use of a lacertid lizard as a model for reptile ecotoxicology studies: part...

Amaral, Maria José; Bicho, Rita C.; Carretero, Miguel A.; Sanchez-Hernandez, Juan C.; Faustino, Augusto M. R.; Soares, Amadeu M. V. M.; Mann, Reinier M.

As part of a wider study examining the impacts of corn pesticides on lacertid lizards in north-western Portugal, we examined various physiological, biochemical, and histological biomarkers of exposure and effect among field populations of Podarcis bocagei. Biomarkers included body condition index, standard metabolic rate, locomotor performance, parasitization, glutathione oxidative pathways and related enzyme a...


The influence of metal speciation on the bioavailability and sub-cellular distr...

Calhoa, Filipa Calhôa; Monteiro, Marta S.; Soares, Amadeu M. V. M.; Mann, Reinier M.

Cadmium is a non-essential toxic metal that is able to bioaccumulate in both flora fauna and has the potential to biomagnify in some food chains. However, the form in which cadmium is presented to consumers can alter the bioavailability and possibly the internal distribution of assimilated Cd. Previous studies in our laboratory highlighted differences in Cd assimilation among isopods when they were provided wit...


Does subcellular distribution in plants dictate the trophic bioavailability of ...

Monteiro, Marta S.; Santos, Conceição; Soares, Amadeu M. V. M.; Mann, Reinier M.

The present study examined how subcellular partitioning of Cd in plants with different strategies to store and detoxify Cd may affect trophic transfer of Cd to the isopod Porcellio dilatatus. The plant species used were Lactuca sativa, a horticultural metal accumulator species; Thlaspi caerulescens, a herbaceous hyperaccumulator species; and the nonaccumulator, T. arvense. Taking into account that differences i...


Bioaccumulation of Cd by a European lacertid lizard after chronic exposure to C...

Mann, Reinier M.; Sánchez-Hernández, Juan C.; Serra, Elisabete A.; Soares, Amadeu M. V. M.

Apart from analyses for elemental contaminants in field collected specimens, very little is known about the assimilation, accumulation and toxic effects of inorganic contaminants in reptiles. This study examined the chronic accumulation of Cd in a European lacertid lizard (Podarcis carbonelli) following dietary provision of an environmentally realistic concentration of Cd for 21 weeks. Lizards were provided wit...


Evaluation of cadmium genotoxicity in Lactuca sativa L. using nuclear microsate...

Monteiro, Marta; Santos, Conceição; Mann, Reinier M.; Soares, Amadeu M. V. M.; Lopes, Tina

Cadmium (Cd) is a non-essential element and is a widespread environmental pollutant. Exposure to cadmium can result in cytotoxic, carcinogenic and mutagenic effects. Mutagenesis is indicative of genetic instability and can be assayed using microsatellites. Microsatellites or simple sequence repeats (SSRs) are composed of tandem repeats of short sequence motifs (1-6 bp) that are polymorphic, mainly in the number...


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