8 documents found, page 1 of 1
Organoids of the male reproductive system: Challenges, opportunities, and their...
Patrício, Daniela; Santiago, Joana; Mano, João F; Fardilha, MargaridaOrganoids are units of function of a given organ able to reproduce, in culture, a biological structure similar in architecture and function to its counterpart in vivo. Today, it is possible to develop an organoid from a fragment of tissue, a stem cell located in an adult organ, an embryonic stem cell, or an induced pluripotent stem cell. In the past decade, many organoids have been developed which mimic stomach...
Chitosan-Based Biomaterials: Insights into Chemistry, Properties, Devices, and ...
Petroni, Simona; Tagliaro, Irene; Antonini, Carlo; D'Arienzo, Massimiliano; Orsini, Sara Fernanda; Mano, João F; Brancato, Virginia; Borges, JoãoChitosan is a marine-origin polysaccharide obtained from the deacetylation of chitin, the main component of crustaceans' exoskeleton, and the second most abundant in nature. Although this biopolymer has received limited attention for several decades right after its discovery, since the new millennium chitosan has emerged owing to its physicochemical, structural and biological properties, multifunctionalities an...
Recent Developments in Layer-by-Layer Assembly for Drug Delivery and Tissue Eng...
Borges, João; Zeng, Jinfeng; Liu, Xi Qiu; Chang, Hao; Monge, Claire; Garot, Charlotte; Ren, Ke-Feng; Machillot, Paul; Vrana, Nihal E; Lavalle, PhilippeSurfaces with biological functionalities are of great interest for biomaterials, tissue engineering, biophysics, and for controlling biological processes. The layer-by-layer (LbL) assembly is a highly versatile methodology introduced 30 years ago, which consists of assembling complementary polyelectrolytes or biomolecules in a stepwise manner to form thin self-assembled films. In view of its simplicity, compati...
Bioengineered hierarchical bonelike compartmentalized microconstructs using nan...
Bjørge, Isabel M.; Sousa, Bárbara M. de; Patrício, Sónia G.; Silva, Ana Sofia; Nogueira, Liebert P.; Santos, Lúcia F.; Vieira, Sandra I.Fabrication of vascularized large-scale constructs for regenerative medicine remains elusive since most strategies rely solely on cell self-organization or overly control cell positioning, failing to address nutrient diffusion limitations. We propose a modular and hierarchical tissue-engineering strategy to produce bonelike tissues carrying signals to promote prevascularization. In these 3D systems, disc-shaped...
Bone physiology as inspiration for tissue regenerative therapies
Lopes, Diana; Martins-Cruz, Cláudia; Oliveira, Mariana B; Mano, João FThe development, maintenance of healthy bone and regeneration of injured tissue in the human body comprise a set of intricate and finely coordinated processes. However, an analysis of current bone regeneration strategies shows that only a small fraction of well-reported bone biology aspects has been used as inspiration and transposed into the development of therapeutic products. Specific topics that include int...
Bioinstructive Naringin-Loaded Micelles for Guiding Stem Cell Osteodifferentiation
Lavrador, Pedro; Gaspar, Vítor M; Mano, João FNaringin is a naturally occurring flavanone with recognized neuroprotective, cardioprotective, anti-inflammatory, and antiosteoporotic properties. Herein, the delivery of Naringin-loaded methoxy-poly(ethylene glycol)-maleimide-thiol-poly(l-lactide) (mPEGMSPLA) diblock polymeric micelles to human adipose-derived stem cells (hASCs) with the aim to augment its pro-osteogenic effect in these cells is reported for...
Bioinspired bone therapies using naringin: applications and advances
Lavrador, Pedro; Gaspar, Vítor M.; Mano, João FThe use of natural compounds for treating chronic bone diseases holds remarkable potential. Among these therapeutics, naringin, a flavanone glycoside, represents one of the most promising candidates owing to its multifaceted effect on bone tissues. This review provides an up-to-date overview on naringin applications in the treatment of bone disorders, such as osteoporosis and osteoarthritis, and further highlig...
Cell-Adhesive Bioinspired and Catechol-Based Multilayer Freestanding Membranes ...
Sousa, Maria P; Mano, João FMussels are marine organisms that have been mimicked due to their exceptional adhesive properties to all kind of surfaces, including rocks, under wet conditions. The proteins present on the mussel's foot contain 3,4-dihydroxy-l-alanine (DOPA), an amino acid from the catechol family that has been reported by their adhesive character. Therefore, we synthesized a mussel-inspired conjugated polymer, modifying the b...