163 documents found, page 1 of 17
Single-cell liquid-core microcapsules for biomedical applications
Pires-Santos, Manuel; Carreira, Mariana; Morais, Bruno P.; Perfeito, Francisca G.; Oliveira, Mariana B.; Monteiro, Cátia F.; Nadine, Sara; Mano, João F.More recently, single-cell encapsulation emerged as a promising field in biomedicine due to its potential applications, in cell analysis and therapy. Traditional techniques involve embedding cells in crosslinked polymers to create continuous microgels, suitable mainly for adherent cells, or encapsulating them in droplets for only short-term analysis, due to their instability. In this study, we developed a metho...
Thiol-ene click chemistry: enabling 3D printing of natural-based inks for biome...
Malafaia, Andreia P.; Sobreiro-Almeida, Rita; Rodrigues, João M. M.; Mano, João F.Over the last decade, 3D bioprinting has gained increasing popularity, being a technique capable of producing well-defined tissue-like structures. One of its most groundbreaking features is the ability to create personalized therapies tailored to the specific demands of individual patients. However, challenges including the selection of materials and crosslinking strategies, still need to be addressed to enhanc...
Osteogenic differentiation triggered by intracellular magnetoelectric stimulati...
Mendes, Maria C.; Martins, Elisa A.G.; Chernozem, Roman V.; Chernozem, Polina V.; Custódio, Catarina C.; Surmenev, Roman A.; Kholkin, Andrei L.Magnetoelectric nanoparticles (MENPs), combining a magnetostrictive core with a piezoelectric shell, offer a promising route for remote-controlled biomedical applications by converting external magnetic fields into electric cues. However, the clinical translation of these materials remains limited due to the toxicity of high-performance piezoelectric materials, which typically contain lead. Previously, we devel...
Modeling 3D tumor invasiveness to modulate macrophage phenotype in a human-base...
Monteiro, Cátia F.; Almeida, Catarina R.; Custódio, Catarina A.; Mano, João F.The immune system is a pivotal player in determining tumor fate, contributing to the immunosuppressive microenvironment that supports tumor progression. Considering the emergence of biomaterials as promising platforms to mimic the tumor microenvironment, human platelet lysate (PLMA)-based hydrogel beads are proposed as 3D platforms to recapitulate the tumor milieu and recreate the synergistic tumor-macrophage c...
Human chorionic membrane-derived tunable hydrogels for vascular tissue engineer...
Martins, Elisa A. G.; Deus, Inês A.; Gomes, Maria C.; Silva, Ana S.; Mano, João F.; Custódio, Catarina A.One of the foremost targets in the advancement of biomaterials to engineer vascularized tissues is not only to replicate the composition of the intended tissue but also to create thicker structures incorporating a vascular network for adequate nutrients and oxygen supply. For the first time, to the best of current knowledge, a clinically relevant biomaterial is developed, demonstrating that hydrogels made from ...
Encapsulation of pristine and silica-coated human adipose-derived mesenchymal s...
Maciel, Marta M.; Hassani Besheli, Negar; Correia, Tiago R.; Mano, João F.; Leeuwenburgh, Sander C. G.Colloidal gels assembled from gelatin nanoparticles (GNPs) as particulate building blocks show strong promise to solve challenges in cell delivery and biofabrication, such as low cell survival and limited spatial retention. These gels offer evident advantages to facilitate cell encapsulation, but research on this topic is still limited, which hampers our understanding of the relationship between the physicochem...
Unveiling the potential of single‐cell encapsulation in biomedical applications...
Pires‐Santos, Manuel; Nadine, Sara; Mano, João F.The encapsulation of single cells has emerged as a promising field in recent years, owing to its potential applications in cell-based therapeutics, bioprinting, in vitro cell culture, high-throughput screening, and diagnostics. Single-cell units offer several advantages, including compatibility with standard imaging techniques, superior diffusion rates, and lower material-to-cell volume ratios. They also serve ...
Shape-versatile fixed cellular materials for multiple target immunomodulation
Sousa, Ana Rita; Cunha, Ana F.; Santos-Coquillat, Ana; Estrada, Beatriz Hernaez; Spiller, Kara L.; Barão, Marta; Rodrigues, Artur Filipe; Simões, SusanaTherapeutic cells are usually administered as living agents, despite the risks of undesired cell migration and acquisition of unpredictable phenotypes. Additionally, most cell-based therapies rely on the administration of single cells, often associated with rapid in vivo clearance. Three-dimensional cellular materials may be useful to prolong the effect of cellular therapies and offer the possibility of creatin...
In-bath 3D printing of anisotropic shape-memory cryogels functionalized with bo...
Castanheira, Edgar J.; Monteiro, Luís P. G.; Gaspar, Vítor M.; Correia, Tiago R.; Rodrigues, João M. M.; Mano, João F.Cryogels exhibit unique shape memory with full recovery and structural stability features after multiple injections. These constructs also possess enhanced cell permeability and nutrient diffusion when compared to typical bulk hydrogels. Volumetric processing of cryogels functionalized with nanosized units has potential to widen their biomedical applications, however this has remained challenging and relatively...
Acidochromic free-standing multilayered chitosan-pyranoflavylium/alginate membr...
Cunha, Mariana; Freitas, Victor de; Borges, João; Mano, João F.; Rodrigues, João M. M.; Cruz, LuísFood smart packaging has emerged as a promising technology to address consumer concerns regarding food conservation and food safety. In this context, we report the rational design of azide-containing pyranoflavylium-based pH-sensitive dye for subsequent click chemistry conjugation toward a chitosan-modified alkyne. The chitosan-pyranoflavylium conjugate was characterized by infrared (ATR-FTIR), ultraviolet−visi...