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How cells and biomaterials from natural origin are revolutionizing tissue engin...
Mano, João F.Tissue engineering and regenerative medicine aim to restore damaged tissues by combining human cells with biomaterials that provide structure and biological cues. After decades of progress from early scaffold-based approaches to today’s biofabrication methods, the field is moving toward therapies that are more precise, more patient-specific, and more biologically faithful. This article presents the key ingredie...
Encapsulation of pristine and silica-coated human adipose-derived mesenchymal s...
Maciel, Marta M.; Hassani Besheli, Negar; Correia, Tiago R.; Mano, João F.; Leeuwenburgh, Sander C. G.Colloidal gels assembled from gelatin nanoparticles (GNPs) as particulate building blocks show strong promise to solve challenges in cell delivery and biofabrication, such as low cell survival and limited spatial retention. These gels offer evident advantages to facilitate cell encapsulation, but research on this topic is still limited, which hampers our understanding of the relationship between the physicochem...
The european polysaccharide network of excellence (EPNOE) research roadmap 2040...
Gericke, Martin; Amaral, Adérito J.R.; Budtova, Tatiana; De Wever, Pieter; Groth, Thomas; Heinze, Thomas; Höfte, Herman; Huber, Anton; Ikkala, OlliPolysaccharides are among the most abundant bioresources on earth and consequently need to play a pivotal role when addressing existential scientific challenges like climate change and the shift from fossil-based to sustainable biobased materials. The Research Roadmap 2040 of the European Polysaccharide Network of Excellence (EPNOE) provides an expert's view on how future research and development strategies nee...
Photo‐compartmentalized decellularized matrix‐hyaluronan hybrid units for pancr...
Monteiro, Maria V.; Henriques-Pereira, Margarida; Neves, Bruno M.; Duarte, Iola D.; Gaspar, Vítor M.; Mano, João F.Tissue-derived decellularized extracellular matrix (dECM) provides unique biomolecular cues for bioengineering 3D tumor models with increasingly physiomimetic features. This biomimicry potential can be expanded by combining dECM with key components of native tissues, such as glycosaminoglycans (GAGs). Yet, this combination remains highly challenging, often requiring complex non-natural chemical modifications of...
A supramolecular injectable methacryloyl chitosan-tricine-based hydrogel with 3...
Ouro, Pedro M. S.; Costa, Dora C. S.; Amaral, Adérito J. R.; Mano, João F.Printable hydrogels have attracted significant attention as versatile, tunable, and spatiotemporally controlled biomaterials for tissue engineering (TE) applications. Several chitosan-based systems are reported presenting low or no solubility in aqueous solutions at physiological pH. Herein, a novel neutrally charged, biomimetic, injectable, and cytocompatible dual-crosslinked (DC) hydrogel system based on a do...
Reversible imine crosslinking in waterborne self-healing polymer coatings
Martins, Tiago D.; Viciosa, M. Teresa; Oliveira, Mariana B.; Fernandes, Auguste; Mano, João F.; Baleizão, Carlos; Farinha, José Paulo S.Waterborne polymer coatings have the potential to address the environmental concerns associated with solvent based systems. To improve their performance without using volatile organic compounds, we propose a new approach based on reconfigurable covalent crosslinking that provides mechanical resistance and self-healing properties. The new waterborne polymer coatings are based on mixtures of aldehyde- and amine-f...
Biomaterials of human source for 3D printing strategies
Maia, João Rocha; Sobreiro-Almeida, Rita; Cleymand, Franck; Mano, João F.Three-dimensional printing has risen in recent years as a promising approach that fast-tracked the biofabrication of tissue engineering constructs that most resemble utopian tissue/organ replacements for precision medicine. Additionally, by using human-sourced biomaterials engineered towards optimal rheological proprieties of extrudable inks, the best possible scaffolds can be created. These can encompass nativ...
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cel...
Ghasemzadeh-Hasankolaei, Maryam; Miranda, João M.; Correia, Clara R.; Mano, João F.It is essential to design a multifunctional well-controlled platform to transfermechanical cues to the cells in different magnitudes. This study introduces aplatform, a miniaturized bioreactor, which enables to study the effect of shearstress in microsized compartmentalized structures. In this system, thewell-established cell encapsulation system of liquefied capsules (LCs) is usedas microbioreactors in which t...
Self-supporting hyaluronic acid-functionalized G-quadruplex-based perfusable mu...
Sousa, Vera; Amaral, Adérito J. R.; Castanheira, Edgar J.; Marques, Igor; Rodrigues, João M. M.; Félix, Vítor; Borges, João; Mano, João F.Dynamic G-quadruplex supramolecular hydrogels have aroused great interest in a broad range of bioapplications. However, neither the development of native extracellular matrix (ECM)-derived natural biopolymer-functionalized G-quadruplex hydrogels nor their use to create perfusable self-supporting hydrogels has been explored to date, despite their intrinsic potential as carrier vehicles of therapeutic agents, or ...
Supramolecular presentation of bioinstructive peptides on soft multilayered nan...
Lopes, Maria; Torrado, Marília; Barth, Daryl; Santos, Sofia D.; Sever-Bahcekapili, Melike; Tekinay, Ayse B.; Guler, Mustafa O.; Cleymand, FranckPeptide amphiphiles (PAs) have emerged as effective molecular building blocks for creating self-assembling nanobiomaterials for multiple biomedical applications. Herein, we report a straightforward approach to assemble soft bioinstructive platforms to recreate the native neural extracellular matrix (ECM) aiming for neuronal regeneration based on the electrostatic-driven supramolecular presentation of laminin-de...