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Response of thymus lotocephalus in vitro cultures to drought stress and role of...

Mansinhos, Inês; Gonçalves, Sandra; Rodríguez-Solana, Raquel; Duarte, Hugo; Ordóñez-Díaz, José Luis; Moreno-Rojas, José Manuel; Romano, Anabela

The impact of drought stress induced by polyethylene glycol (PEG) on morphological, physiological, (bio)chemical, and biological characteristics of Thymus lotocephalus López and Morales shoot cultures have been investigated, as well as the potential of iron oxide nanoparticles, salicylic acid, and methyl jasmonate (MeJA) as alleviating drought stress agents. Results showed that PEG caused oxidative stress in a ...


Influence of wine pH and ethanol content on the fining efficacy of proteins fro...

Baca-Bocanegra, Berta; Gonçalves, Sandra; Nogales-Bueno, Julio; Mansinhos, Inês; Heredia, Francisco José; Hernández-Hierro, José Miguel; Romano, A.

Wine color and limpidity are important aspects of consumer preferences. The alteration of these parameters can damage wine’s appearance but also its mouthfeel characteristics due to its relationship with attributes such as bitterness and astringency. Fining is a practice usually used in enology to modulate undesirable wine organoleptic attributes. However, there are several factors that influence this technique...


Impact of temperature on Phenolic and Osmolyte contents in In Vitro cultures an...

Mansinhos, Inês; Gonçalves, Sandra; Rodríguez Solana, Raquel; Ordóñez-Díaz, José Luis; Moreno-Rojas, José Manuel; Romano, Anabela

In this study, in vitro cultures and micropropagated plants of two Mediterranean aromatic plants, Lavandula viridis L’Hér and Thymus lotocephalus López and Morales, were exposed to different temperatures (15, 20, 25, and 30 ◦C). The effect of temperature on the levels of hydrogen peroxide (H2O2 ), lipid peroxidation, and osmoprotectants (proline, soluble sugars, and soluble proteins), as well as on the phenolic...


Ultrasonic-assisted extraction and natural deep eutectic solvents combination: ...

Mansinhos, Inês; Gonçalves, Sandra; Rodríguez Solana, Raquel; Ordóñez-Díaz, José Luis; Moreno-Rojas, José Manuel; Romano, A.

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with con...


Impact of metallic nanoparticles on In vitro culture, phenolic profile and biol...

Gonçalves, Sandra; Mansinhos, Inês; Rodríguez-Solana, Raquel; Pereira-Caro, Gema; Moreno-Rojas, José Manuel; Romano, Anabela

Nanoparticles (NPs) recently emerged as new chemical elicitors acting as signaling agents affecting several processes in plant metabolism. The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe3O4 ) NPs (<100 nm) at different concentrations (1, 5 and 10 mg/L) to the culture media on several morphological, physiological and -biochemical param...


Exploring the biotechnological value of marine invertebrates: a closer look at ...

Pan, Yu-Lun; Rodrigues, Maria João; Pereira, Catarina; Engrola, Sofia; Colen, R.; Mansinhos, Inês; Romano, Anabela; Andrade, Paula B.; Fernandes, Fátima

<i>Sabella spallanzanii</i> and <i>Microcosmus squamiger</i> were profiled for proximate composition, minerals, amino acids, fatty acids (FA), carotenoids, radical scavenging activity on the 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical, oxygen radical absorbance capacity (ORAC) and iron and copper chelating properties. <i>Microcosmus squamiger</i> had the highest level of moisture and crude protein, <i>S. spal...


Influence of elaboration process on chemical, biological, and sensory character...

Rodríguez Solana, Raquel; Esteves, Eduardo; Mansinhos, Inês; Gonçalves, Sandra; Perez-Santin, Efren; Galego, Ludovina; Romano, Anabela

BACKGROUND: Mentha pulegium is an aromatic and medicinal plant used in different Mediterranean herb-based liqueurs, whose phytochemical composition depends on the elaboration technique used. The aim of this work was to investigate differences in the sensory, chemical, and biological characteristics of pennyroyal liqueurs elaborated by hydro-alcoholic (HI) and aqueous (AI) infusions, percolation (P), maceration ...


Greener is better: first approach for the use of natural deep eutectic solvents...

Rukavina, Iva; Rodrigues, Maria João; Pereira, Catarina; Mansinhos, Inês; Romano, Anabela; Ślusarczyk, Sylwester; Matkowski, Adam; Custódio, Luísa

In this study, natural deep eutectic solvents (NADES) formed by choline chloride (ChCl), su crose, fructose, glucose, and xylose, were used to extract antioxidants from the halophyte Polygonum maritimum L. (sea knotgrass) and compared with conventional solvents (ethanol and acetone). NADES and conventional extracts were made by an ultrasound-assisted procedure and evaluated for in vitro antioxidant properties b...


Phenolic profile, antioxidant activity and enzyme inhibitory capacities of frui...

Djaoudene, Ouarda; Mansinhos, Inês; Gonçalves, Sandra; Jose Jara-Palacios, M.; Bey, Mostapha Bachir; Romano, Anabela

The aim of the present study was to evaluate the influence of the digestive process (gastric and intestinal phases) on the stability of the individual and total phenolic (TPC) and flavonoid (TFC) contents, antioxidant activity and enzymes inhibitory potential of extracts from date fruits (pulp) and seeds from eight Algerian cultivars of date palm (Phoenix dactylifera). The obtained results showed a considerable...


Elicitation improves rosmarinic acid content and antioxidant activity in Thymus...

Gonçalves, Sandra; Mansinhos, Inês; Rodríguez Solana, Raquel; Perez-Santin, Efren; Coelho, Natacha; Romano, Anabela

Thymus lotocephalus G. Lopez & R. Morales is an aromatic species endemic to the south of Portugal with medicinal properties. The aim of this work was to evaluate the effect of elicitors, cytokinin, and different sucrose concentrations on chemical composition and antioxidant activity of extracts from in vitro regenerated shoots. Elicitors (YE: yeast extract, salicylic acid, and AgNO3) were added directly to the ...


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