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Cellular and biochemical alterations induced by diesel oil in the brown alga Sa...

Oliveira, Eva Regina; Ramlov, Fernanda; Schmidt, Éder Carlos; Tomazi, Débora; Bauer, Claudia Marlene; Pilatti, Fernanda K.; Moresco, Rodolfo

The effect of the in vitro acute exposure to diesel oil (0.001%, 0.01%, 0.1%, and 1%, v/v) has been evaluated in the brown seaweed Sargassum cymosum var. stenoplhyllum by determining the contents of photosynthetic pigments, phenolic compounds, and morphological and cellular changes. In vitro cultures of S. cymosum were exposed to diesel oil for 30min, 1h, 12h, and 24h under laboratory-controlled conditions. Chl...


Evaluation of low-cost, easy-to-prepare protein diets using beer yeast, sugar c...

Müller, Mara Rúbia Romeu Pinto; Nunes, Aline; Maraschin, Marcelo; Araújo, Denise Nunes; Pereira Leivas Leite, Fábio

The aim of the study was to evaluate different diets for Apis mellifera in three regions of Santa Catarina, Brazil. The bees received the diets weekly, for 60 days. The treatments used and the corresponding crude protein contents were: D1 – sugar syrup; D2 – commercial feed (24.4%); D3 – sugarcane yeast + commercial sugar (17.0%); D4 – beer yeast + commercial sugar (14.9%); D5 – sugarcane yeast + textured soy p...

Date: 2022   |   Origin: Oasisbr

The influence of recent Brazilian policy and legislation on increasing bee mort...

Nunes, Aline; Schmitz, Caroline; Moura, Sidnei; Maraschin, Marcelo

The decline in bee communities in recent years has been a major concern worldwide. The increase in the mortality of these pollinators is related to several factors, the main one being the intensive use of pesticides in agricultural crops. Brazil is the world leader in use of pesticides since 2008 and recent changes in legislation have facilitated the commercialization of certain pesticides whose marketing has b...

Date: 2021   |   Origin: Oasisbr

Campomanesia spp. native fruits as potential source of health-promoting compounds

Verruck, Silvani; Cunha Junior, Anildo; Maraschin, Marcelo; Fronza, Nei; Budke, Jean Carlos; Hassemer, Guilherme de Souza; Prudencio, Elane Schwinden

Campomanesia xanthocarpa var. littoralis, Campomanesia xanthocarpa (Berg), and Campomanesia eugenioides are native fruit plants found in Brazil. Due to the scarce number of controlled scientific studies comparing different native Campomanesia species, this study sought to determine their bioactive compounds and antioxidant properties. C. eugenioides proved to be a rich source of total phenolic compounds, also s...

Date: 2021   |   Origin: Oasisbr

NMRFinder: a novel method for 1D 1H-NMR metabolite annotation

Cardoso, Sara; Cabral, Débora; Maraschin, Marcelo; Rocha, Miguel

Introduction: Methods for the automated and accurate identification of metabolites in 1D 1H-NMR samples are crucial, but this is still an unsolved problem. Most available tools are mainly focused on metabolite quantification, thus limiting the number of metabolites that can be identified. Also, most only use reference spectra obtained under the same specific conditions of the target sample, limiting the use of ...


Development of computational tools for the analysis of 2D-nuclear magnetic reso...

Pereira, Bruno; Maraschin, Marcelo; Rocha, Miguel

Metabolomics is one of the omics' sciences that has been gaining a lot of interest due to its potential on correlating an organism's biochemical activity and its phenotype. The main techniques that collect data are based on mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy. The last one has the advantage of analysing a sample in vivo without damaging it and while its sensitivity is pointed out...


Banana (Musa spp.) as a source of bioactive compounds for health promotion

Lopes, Susane; Borges, Cristine Vanz; Cardoso, Sara; Rocha, Miguel; Maraschin, Marcelo

Banana and plantain are monocotyledonous plants belonging to the genus Musa, family Musaceae, and order Zingiberales. All around the world, the importance of banana plant increases with its different applications in pharmaceutical and food industries and in other biotechnologically related processes. This chapter provides an overview of bioactive compounds in banana and biological activities of banana's seconda...


Botanical source investigation and evaluation of the effect of seasonality on B...

Tomazzoli, Maíra Maciel; Zeggio, Amélia Regina Somensi; Pai Neto, Remi Dal; Specht, Leandro; Costa, Christopher; Rocha, Miguel; Yunes, Rosendo Augusto

Bees generally use different botanical sources of resins for the production of propolis. The elucidation of botanical sources of propolis and identification of the effects of seasonality on the chemical profile of propolis are recognized as two important aspects in the development of a high quality product. Thus, our objective was to elucidate the botanical source and identify the effect of the seasons on the c...


17α-ethinylestradiol analysis of endo- and exometabolome of Ulva lactuca (chlor...

Cabral, Debora; Cardoso, Sara; Rocco, Silvana; Sforça, Maurício; Hengeltraub, Simone Fanan; Bauer, Cláudia; Rocha, Miguel; Maraschin, Marcelo

The uptake of 17α-ethinylestradiol (EE2) by the green alga Ulva lactuca and its effects on the metabolome of that macroalgae have been investigated using 1H-NMR spectroscopy. To this end, U. lactuca in vitro cultures have been acutely (48 h) exposed to the estrogen and both, endo- and exometabolome were investigated. Bioinformatic analyses were performed to integrate and analyze endo- and exometabolic profiles ...


WebSpecmine: a website for metabolomics data analysis and mining

Cardoso, Sara; Afonso, T.; Maraschin, Marcelo; Rocha, Miguel

Metabolomics data analysis is an important task in biomedical research. The available tools do not provide a wide variety of methods and data types, nor ways to store and share data and results generated. Thus, we have developed WebSpecmine to overcome the aforementioned limitations. WebSpecmine is a web-based application designed to perform the analysis of metabolomics data based on spectroscopic and chromatog...


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