4 documents found, page 1 of 1
Serological reactivity of different antigenic preparations of Leishmania (Leish...
Gomes-Silva,Adriano; Souza,Maria Aparecida; Afonso-Cardoso,Sandra Regina; Andrade,Lívia Resende; Dietze,Reynaldo; Lemos,Elenice; Belli,AlejandroTotal antigen from Leishmania (Leishmania) amazonensis and isolates from the Leishmania braziliensis complex, along with their respective antigenic fractions obtained by affinity chromatography on concanavalin-A-Sepharose and jacalin-agarose columns evaluated using immunoenzymatic ELISA assay. For this, serum samples from 229 patients were used, grouped as American tegmental leishmaniasis (nº=58), visceral leis...
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens ...
Ferreira,Ana Paula; Côrrea,Thadeu; Cunha,Rosângela; Marques,Marcos José; Montesano,Maria Angela; Souza,Maria Aparecida; Teixeira,Henrique CoutoIn this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosi...
Isolation and partial characterization of a D-galactose-binding lectin from the...
Souza,Maria Aparecida; Amâncio-Pereira,Francielle; Cardoso,Cristina Ribeiro Barros; Silva,Adriano Gomes da; Silva,Edmar Gomes; Andrade,Lívia ResendeA lectin from the latex of Synadenium carinatum was purified by affinity chromatography on immobilized-D-galactose-agarose and shown to be a potent agglutinin of human erythrocytes. The haemagglutination of human red cells was inhibited by 3.0 mM N-acetyl-D-galactopyranoside, 6.3 mM methyl-beta-D-galactopyranoside, 50 mM methyl-alpha-D-galactopyranoside and 50 mM D-fucose but not by L-fucose, demonstrating an a...
Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
Yan,Xu; Souza,Maria Aparecida; Pontes,Marcela Z. R.; Vitolo,Michele; Pessoa Júnior,AdalbertoFrom analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aq...