4 documents found, page 1 of 1
The Octahydroindene Carboxyl Substructure from Dihydrobetulinic Acid is Essenti...
Rocha,Camila A.; Sanches,Paulo R. S.; Marchetto,Reinaldo; Zottis,AdersonThe dihydrobetulinic acid is a known competitive inhibitor of topoisomerase IB from Leishmania donovani, a validated target for developing new antileishmanial drugs. However, its binding mode and interaction pocket have not been established yet. We combined docking and molecular dynamics simulations to identify the most probable binding pocket. Our best model strongly suggests a cavity involving the residues ar...
Biossíntese e recentes avanços na produção de celulose bacteriana
Donini,Ígor A. N.; Salvi,Denise T. B. De; Fukumoto,Fabiana K.; Lustri,Wilton R.; Barud,Hernane S.; Marchetto,Reinaldo; Messaddeq,YounesO presente trabalho discute os recentes avanços na biossíntese e na produção de celulose bacteriana (CB) pela gram-negativa, aeróbia e aceto-ácida Gluconacetobacter. xylinus. A CB se difere de seu par vegetal, principalmente devido ao seu caráter de fibras nanométricas, contra o caráter micrométrico da vegetal, são extruídas através da parede celular de G. xylinus, com isso sua estrutura macroscópica é mecanica...
Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA...
Cotrim,Camila Aparecida; Garrido,Saulo Santesso; Trovatti,Eliane; Marchetto,ReinaldoToxin-antitoxin (TA) systems contribute to plasmid stability by a mechanism called post-segregational killing. The ccd was the first TA system to be discovered with CcdB being the toxin and CcdA the antitoxin. CcdA, an 8.3 kDa protein, interacts with CcdB (11.7 kDa), preventing the cytotoxic activity of CcdB on the DNA gyrase. As an approach to understanding this interaction, CcdA41, a polypeptide derived from ...
Comparative time-course study of aminoacyl- and dipeptidyl-resin hydrolysis
Jubilut,Guita N.; Marchetto,Reinaldo; Cilli,Eduardo M.; Oliveira,Eliandre; Miranda,Antonio; Tominaga,Mineko; Nakaiee,Clóvis R.The classic hydrolysis procedure for quantification of resin-bound aminoacyl and peptidyl groups with 12 N HCl: propionic acid was reevaluated by studying the influence of the nature of the resin and the resin-bound group. Their stability during acid hydrolysis was dependent on the C-terminal amino acid, and the order of acid stability was Phe > Val > Gly. Otherwise, the dipeptides Ala-Gly, Ala-Val, and Ala-Phe...