10 documents found, page 1 of 1
Alterations in mucosa branched N-glycans lead to dysbiosis and downregulation o...
Rodrigues, CS; Gaifem, J; Pereira, MS; Alves, MF; Silva, M; Padrão, N; Cavadas, B; Moreira-Barbosa, C; Alves, I; Marcos-Pinto, R; Torres, J; Lavelle, AThe perturbation of the symbiotic relationship between microbes and intestinal immune system contributes to gut inflammation and Inflammatory Bowel Disease (IBD) development. The host mucosa glycans (glycocalyx) creates a major biological interface between gut microorganisms and host immunity that remains ill-defined. Glycans are essential players in IBD immunopathogenesis, even years before disease onset. Howe...
Reprogramming CD8+ T-cell Branched N-Glycosylation Limits Exhaustion, Enhancing...
Azevedo, CM; Xie, B; Gunn, WG; Peralta, RM; Dantas, CS; Fernandes-Mendes, H; Joshi, S; Dean, V; Almeida, P; Wilfahrt, D; Mendes, N; Portero, JLT-cell therapies have transformed cancer treatment. While surface glycans have been shown to play critical roles in regulating T-cell development and function, whether and how the glycome influences T cell–mediated tumor immunity remains an area of active investigation. In this study, we show that the intratumoral T-cell glycome is altered early in human colorectal cancer, with substantial changes in branched N...
T-cell branched glycosylation as a mediator of colitis-associated colorectal ca...
Leite-Gomes, E; Silva, MC; Dias, AM; Fernandes, Â; Faria, G; Nogueira, R; Santos-Pereira, B; Fernandes-Mendes, H; Azevedo, CM; Raposo, J; Portero, JLBackground and Aims: Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract, established as a risk factor for colorectal cancer (CRC) development. Long-standing inflammation appears to play a central role in colitis-associated colorectal cancer (CAC). However, the molecular mechanism underlying CAC progression is still elusive. Previous evidence showed that levels of b...
Genetic Variants of the MGAT5 Gene Are Functionally Implicated in the Modulatio...
Pereira, MS; Durães, C; Catarino, TA; Costa, JL; Cleynen, I; Novokmet, M; Krištic, J; Štambuk, J; Conceição-Neto, N; Machado, JC; Marcos-Pinto, ROBJECTIVES: The impact of genetic variants (single nucleotide polymorphisms [SNPs]) in the clinical heterogeneity of ulcerative colitis (UC) remains unclear. We showed that patients with UC exhibit a deficiency in MGAT5 glycogene transcription in intestinal T cells associated with a hyperimmune response. Herein, we evaluated whether MGAT5 SNPs might functionally impact on T cells glycosylation and plasma IgG gl...
Training in endoscopic mucosal resection and endoscopic submucosal dissection: ...
Küttner-Magalhães, R; Dinis-Ribeiro, M; Bruno, MJ; Marcos-Pinto, R; Rolanda, C; Koch, ADINTRODUCTION: Endoscopic mucosal resection and endoscopic submucosal dissection are demanding procedures. This study aims to establish face, content and expert validity of the live porcine model in performing endoscopic mucosal resection, endoscopic submucosal dissection, complication management and to assess it as a training tool. MATERIAL AND METHODS: Tutors and trainees participating in live porcine model en...
Multicenter survey on the use of device-assisted enteroscopy in Portugal
Pinho, R; Mascarenhas-Saraiva, M; Mão-de-Ferro, S; Ferreira, S; Almeida, N; Figueiredo, P; Rodrigues, A; Cardoso, H; Marques, M; Rosa, B; Cotter, JDevice-assisted enteroscopies (DAEs) are recent endoscopic techniques that enable direct endoscopic small-bowel evaluation.
Multicenter survey on the use of device-assisted enteroscopy in Portugal
Pinho, R; Mascarenhas-Saraiva, M; Mão-de-Ferro, S; Ferreira, S; Almeida, N; Figueiredo, P; Rodrigues, A; Cardoso, H; Marques, M; Rosa, B; Cotter, JBACKGROUND: Device-assisted enteroscopies (DAEs) are recent endoscopic techniques that enable direct endoscopic small-bowel evaluation. OBJECTIVE: The objective of this article is to evaluate the implementation of DAEs in Portugal and assess the main indications, diagnoses, diagnostic yield, therapeutic yield and complication rate. METHODS: We conducted a multicenter retrospective series using a national Web-ba...
Helicobacter pylori chronic infection and mucosal inflammation switches the hum...
Magalhães, A; Marcos-Pinto, R; Nairn, A; dela Rosa, M; Ferreira, RM; Junqueira-Neto, S; Freitas, DP; Gomes, J; Oliveira, P; Santos, M; Marcos, NHelicobacter pylori exploits host glycoconjugates to colonize the gastric niche. Infection can persist for decades promoting chronic inflammation, and in a subset of individuals lesions can silently progress to cancer. This study shows that H. pylori chronic infection and gastric tissue inflammation result in a remodeling of the gastric glycophenotype with increased expression of sialyl-Lewis a/x antigens due t...
Dysregulation of T cell receptor N-glycosylation: A molecular mechanism involve...
Dias, A; Dourado, J; Lago, P; Cabral, J; Marcos-Pinto, R; Salgueiro, P; Almeida, CR; Carvalho, S; Fonseca, S; Lima, M; Vilanova, M; Dinis-Ribeiro, MThe incidence of inflammatory bowel disease is increasing worldwide and the underlying molecular mechanisms are far from being fully elucidated. Herein, we evaluated the role of N-glycosylation dysregulation in T cells as a key mechanism in the ulcerative colitis (UC) pathogenesis. The evaluation of the branched N-glycosylation levelsandprofile of intestinalTcell receptor (TCR)wereassessedin colonic biopsies fr...
A multicenter validation of an endoscopic classification with narrow band imagi...
Pimentel-Nunes, P; Dinis-Ribeiro, M; Soares, JB; Marcos-Pinto, R; Santos, C; Rolanda, C; Bastos, RP; Areia, M; Afonso, L; Bergman, J; Sharma, PBACKGROUND AND STUDY AIM: The reliability and external validity of narrow band imaging (NBI) in the stomach have not been described consistently. The aim of the current study was to describe and estimate the accuracy and reliability of a simplified classification system for NBI in the diagnosis of gastric lesions. METHODS: Consecutive patients undergoing NBI endoscopy at two reference centers (n=85, 33% with dy...