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Células da glândula mamária ovina cultivadas in vitro expressam beta-lactoglobu...

Giassetti, Mariana Ianello; Barros, Flávia Regina Oliveira de; Mendes, Camilla Mota; Goissis, Marcelo Demarchi; Maria, Fernanda Sevciuc

The expression of milk proteins in vitro is essential to exploit the mammary gland cells as a biological model. Enzymatic tissue disaggregation has been widely used to establish mammary cell culture, but its effect in long-term ovine mammary cell culture is not completely elucidated. This study aimed at comparing mechanical/enzymatic and mechanical dissociation methods to establish ovine mammary cell culture. W...

Date: 2017   |   Origin: Oasisbr
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