6 documents found, page 1 of 1
The Aspergillus fumigatus Mismatch Repair MSH2 Homolog Is Important for Virulen...
Dos Reis, Thaila Fernanda; Silva, Lilian Pereira; de Castro, Patrícia Alves; do Carmo, Rafaela Andrade; Marini, Marjorie MendesThe genetic stability of every living organism depends on accurate DNA replication and repair systems. Here, we investigated the Aspergillus fumigatusMSH2 mismatch repair (MMR) gene MshA and how it impacts virulence and the evolution of azole resistance. We examined mshA gene variation in 62 environmental and clinical A. fumigatus strains. We have observed 12 strains with variants (18.2%), and 8 strains among t...
The influence of genetic stability on Aspergillus fumigatus virulence and azole...
Reis, Thaila Fernanda dos; Silva, Lilian Pereira; Castro, Patrícia Alves; Lima, Pollyne Borborema Almeida de; Carmo, Rafaela Andrade doGenetic stability is extremely important for the survival of every living organism, and a very complex set of genes has evolved to cope with DNA repair upon DNA damage. Here, we investigated the Aspergillus fumigatus AtmA (Ataxia-telangiectasia mutated, ATM) and AtrA kinases, and how they impact virulence and the evolution of azole resistance. We demonstrated that A. fumigatus atmA and atrA null mutants are hap...
Comparative genomics of the major fungal agents of human and animal Sporotricho...
Teixeira, Marcus de Melo; Almeida, Luiz G. P. de; Barreira, Paula Kubitschek; Alves, Fernanda L.; Kioshima, Érika Seki; Abadio, Ana Karina RodriguesBackground: The fungal genus Sporothrix includes at least four human pathogenic species. One of these species, S. brasiliensis, is the causal agent of a major ongoing zoonotic outbreak of sporotrichosis in Brazil. Elsewhere, sapronoses are caused by S. schenckii and S. globosa. The major aims on this comparative genomic study are: 1) to explore the presence of virulence factors in S. schenckii and S. brasiliens...
Identification and characterization of expressed retrotransposons in the genome...
Soares, Marco Aurélio; Araújo, Roberta Amália de Carvalho; Marini, Marjorie Mendes; Oliveira, Luciana Márcia de; Lima, Leonardo Gomes deBackground: Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transpos...
Identification and characterization of Tc1/ mariner-like DNA transposons in gen...
Marini, Marjorie Mendes; Zanforlin, Tamiris; Santos, Patrícia Campi; Barros, Roberto Rudge de Moraes; Guerra, Anne Cristina Pinheiro; Puccia, RosanaBackground: Paracoccidioides brasiliensis (Eukaryota, Fungi, Ascomycota) is a thermodimorphic fungus, the etiological agent of paracoccidioidomycosis, the most important systemic mycoses in Latin America. Three isolates corresponding to distinct phylogenetic lineages of the Paracoccidioides species complex had their genomes sequenced. In this study the identification and characterization of class II transposabl...
The repetitive cytoskeletal protein H49 of Trypanosoma cruzi is a calpain-like ...
Galetovic, Alexandra; Souza, Renata Torres de; Valiati, Márcia Regina Machado Santos; Cordero Veas, Esteban Mauricio; Bastos, Izabela Marques DouradoParacoccidioides brasiliensis causes paracoccidioidomycosis, a systemic mycosis in Latin America. Formamidases hydrolyze formamide, putatively plays a role infungal nitrogen metabolism. An abundant 45-kDa protein was identified as the P. brasiliensis formamidase. In this study, recombinant formamidase was over-expressed in bacteria and a polyclonal antibody to this protein was produced. Weidentified a 180-kDa pro...